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NATIONAL RESEARCH PROJECTS


Australia

Sender Institution:

Department of Natural Resources and Environment

Address:

Ovens Research Station, PO Box 235 - Myrtleford VIC., 3737 (Australia)

Tel.:

+61 357 311222

Fax.:

+61 357 311223

Email:

ridleyd@ovens.agvic.gov.au



Research project



Title:

Chestnut Varietal Evaluation

Project leader:

RIDLEY Dan, MCGREGOR Graeme

Starting date:

March 1999

Funding institution:

Horticultural Research and Development Corporation Sydney, Australia

Project objectives

To evaluate promising new lines of chestnuts for a range of physical attributes including; peel, taste, pellice intrusion and disease incidence.

To evaluate two commonly grown varieties over a number of environments to document resultant differences in nut production and nut characteristics.

Project summary and results

Project is still in progress with results anticipated in July 1999.

Austria

Sender Institution:

Hygienicum; Institut für Mikrobiologie & Hygiene-Consulting GmbH

Address:

Andritzer Reichsstr. 44 - 8045 Graz

Tel.:

+43 316 694108

Fax.:

+43 316 694125

Email:

hygienicum@mail.styria.com



Research project



Title:

Biological Control of Chestnut Blight in Austria based on the principle of hypovirulence of Cryphonectria parasitica

Project leader:

STELZL Michael

Participants:

RÜHMER Thomas

Starting date:

October 1998

Duration:

Three years

Funding institution:

Government of Styria

Project summary and results

Three formulations of hypovirulent C. parasitica strains were tested: Maizena-paste, on chestnut wood, suspension. A method should be developed to carry out the healing of sick chestnut stands in agricultural stands but also in forestry. The aim of this research is the development of a method easy to handle and effective in converting the virulent cankers to hypovirulent in Austrian chestnut stands.

Sender Institution:

Austrian Research Centers Seibersdorf

Address:

2444 Seibersdorf

Tel.:

+43 2254 780 3527

Fax.:

+43 2254 780 3653

Email:

eva.wilhelm@arcs.ac.at


roland.schafleitner@arcs.ac.at



Research project



Title:

Biocontrol of Chestnut Blight

Project leader:

WILHELM Eva

Participants:

Biotechnology Unit of the Austrian Research Centers Seibersdorf

Starting date:

November 1998

Duration:

Three years

Funding institutions:

Austrian Federal Ministry of Agriculture and Forestry,


Austrian Federal Ministry of Science and Traffic

Project objectives

Mapping of chestnut blight in Austria, introduce and map hypovirulence, investigate plant defence reactions against Cryphonectria parasitica.

Project summary and results

Current works: collection of fungal strains, conversion to hypovirulence, investigations of defence mechanisms on molecular level.

Related publications

Schafleitner, R. & Wilhelm, E. 1999. Chestnut blight: monitoring the host response with heterologous cDNA probes. Acta Horticulturae, in press.

Schafleitner, R., Buchala, A. & Wilhelm, E. 1999. Class III chitinase expression and salicylic acid accumulation in chestnut (Castanea sativa Mill.) after challenge with hypovirulent and virulent Cryphonectria parasitica (Murr.) Barr. Phyton. (In press).

Sender Institution:

Austrian Research Centers Seibersdorf

Address:

2444 Seibersdorf

Tel.:

+43 2254 780 3527

Fax.:

+43 2254 780 3653

Email:

eva.wilhelm@arcs.ac.at


roland.schafleitner@arcs.ac.at



Research project



Title:

Elucidation of Gene Functions of deciduous tree species

Project leader:

WILHELM Eva

Participants:

Biotechnology Unit of the Austrian Research Centers Seibersdorf

Starting date:

January 1998

Duration:

Three years

Funding institution:

Austrian Federal Ministry of Science and Traffic

Project objectives

Transformation of oak, somatic embryogenesis of chestnut, isolation of stress response genes from chestnut.

Project summary and results

Current works: somatic embryogenesis of chestnut, isolation of stress genes from chestnut.

China

Sender Institution:

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences

Address:

Nanjing 210014, Jiangsu

Tel.:

+86 25 4432128

Fax.:

+86 25 4432074

Email:

jsszzzzz@public1.ptt.js.cn



Research project



Title:

Study on adaptation of excellent cultivars and high yielding culture. Technics of Chinese chestnut in Yi Shu he River plain

Project leader:

LIU Liu

Participants:

Liu Kan-xi, Gu Ying, Lu Cui-hua.

Starting date:

1976

Duration:

1980

Funding institution:

Jiangsu Institute of Botany

Project objectives

Primary selection of excellent cultivars, exploration of the potential productivity and primary sum of the suitable culture technics.

Project summary and results

Seven excellent cultivars have been selected. A mean yield of 5 170.5 kg per ha were produced by six-year old trees in conditions of high dense planting and a highest yield record of over 8 025 kg per ha in the following year.

Related publications

Jiangsu Institute of Botany. 1976. The varieties and cultural techniques of Chinese chestnut in Jiangsu Province. Scientific and Technical Information of Forestry, 3.

Liu Liu. 1982. A study on scheme of Chinese chestnut in Jiangsu Province. Bulletin of the Nanjing Botanical Garden Mem.Sun, Yat sen,1982.

Sender Institution:

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences

Address:

Nanjing 210014, Jiangsu

Tel.:

+86 25 4432128

Fax.:

+86 25 4432074

Email:

jsszzzzz@public1.ptt.js.cn



Research project



Title:

Selection of excellent cultivars of Chinese chestnut

Project leader:

Liu Liu

Participants:

Lin Kan-xi, Zhou Jiu-ya, Bi Hui-chan, Geng Guo-ming, Wang Ben-zhi.

Starting date:

1986

Duration:

Four years (1990)

Funding institution:

State Science and Technology Commission

Project objectives

Selection of excellent cultivars with characteristics of prolificacy and good organoleptic qualities.

Project summary and result

A series of excellent cultivars, Jiu jia zhong, Chu shuhong, Qin za, Duan za, and Jian ding you li, have been recommended.

Related publications

Liu Liu. 1989. A new cultivar of Chinese chestnut-Jian ding you li. Plant Introduction and Acclimatization,1989.

Liu Liu. 1992. A study on germplasm diversity of chestnut and the proposals for its conservation and utilization. Jour. of Plant Resour. & Environ. 1992,1(1).

Zhou, Jiu-ya., Geng, Guo-ming. & Bi Hui-chan, et al. 1994. The comparative test of Chinese chestnut cultivars. Proc. of the Research on Agri. Resour. Utiliz. in Hilly and Mount. areas of Ning-Zheng-Yang.

Liu Liu. 1993. The Germplasm resources of chestnut in China. Proc. of the Inter. Cong. on Chestnut. Spoleto, Italy.

Liu Liu. 1997. Nut quality on Chinese chestnut cultivars. NNGA 1997.

Sender Institution:

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences

Address:

Nanjing 210014, Jiangsu

Tel.:

+86 25 4432128

Fax.:

+86 25 4432074

Email:

jsszzzzz@public1.ptt.js.cn



Research project



Title:

The extension and application of the high yielding culture technique of Chinese chestnut

Project leader:

LIU Liu

Participants:

Zhou Jiu-ya, Bi Hui-chan, Geng Guo-ming, Mao Ai-ning, Liu Qin

Starting date:

1990

Duration:

Five years (1995)

Funding institution:

Jiangsu Science and Technology Commission

Project objectives

Reform of the traditional technics and increasing nut production of Chinese chestnut and development of hilly and mountainous areas.

Project summary and results

The achievements were extended over a wide range in subtropical regions in China and covered an area over 77 000 ha. The increment of nut production was 7-20 times above that of traditional chestnut orchards.

Related publications

Liu Liu. 1998. Progress on the research and production of chestnut in Jiangsu Province and discussion on development of the chestnut industry in China. Proc. of the National Symp. of Chestnut Industry Development.

Liu Liu, Zhou Jiu-ya. 1998. Some Consideration on Chestnut Development in the 21st century in China. Proc. 2nd Symp. on Chestnut.

Liu Liu. 1999. The world chestnut industry and consideration on the development of Chinese chestnut facing the 21st century. Hebei Journal of Forestry and Orchard Research 14 (1).

France

Sender Institution:

Équipe de Glycobiologie Végétale, Institut de Biotechnologie (UPRES 1074)

Address:

123 Avenue Albert Thomas - 87060 Limoges

Tel.:

+33 5 55457660

Fax.:

+33 5 55457386

Email:

gcosta@unilim.fr



Research project



Title:

Investigation of the molecular level, especially for fucosyl-transferase genes

Project leader:

COSTA Guy

Sender Institution:

ENITA de Bordeaux (Ecole Nationale d'Ingenieurs des Travaux Agricoles)

Address:

1, cours du Général de Gaulle BP 201 - 33175 Gradignan

Tel.:

+33 5 57350755

Fax.:

+33 5 57350759

Email:

l-guerin@enitalan.fr



Research project



Title:

Epidemiology of chestnut blight

Project leader:

GUERIN Lucia

Participants:

ROBIN Cécile, GUERIN Lucia

Starting date:

1995

Funding institution:

DGER

Project objectives

Basic knowledge on epidemiology of Cryphonectria parasitica: inoculum production, disponibility, dissemination, infection process.

Related publications

Guérin, L., Bastien, S. & Chauvin, B. 1998. The production and dispersal of ascospores of Cryphonectria parasitica in an orchard in South-Western France. The Second International Symposium on Chestnut. Bordeaux, 19-23 October.

Guérin, L., Bastien, S., Capron, G. & Robin, C. 1999. Seasonal changes in susceptibility of Castanea sativa to virulent and hypovirulent isolates of Cryphonectria parasitica. Cost MC Meeting. Sopron, Hungary, 6-8 May.

Guérin, L., Froidefond, G. & Xu, X. 1999. Dissemination pattern of airborne ascospores of Cryphonectria parasitica casal agent of chestnut blight. European Journal of Plant pathology (Submitted).

Japan

Sender Institution:

National Institute of Fruit Tree Science, Min. Agr. for. Fish.

Address:

305-8605 Tsukuba, Ibaraki

Tel.:

+81 0298 386464

Fax.:

+81 0298 386437

Email:

kkazuo@fruit.affrc.go.jp



Research project



Title:

Breeding of Japanese Chestnut, 6th phase

Project leader:

KOTOBUKI Kazuo

Participants:

SHODA M., SAITO T.

Starting date:

April 1992

Duration:

Eighteen years (2010 March)

Funding institution:

National Institute of Fruit Tree Science

Project objectives

Selection of promising tree(s).

Project summary and results

Four promising clones selected. Local adaptability test will start in next growing season.

Greece

Sender Institution:

Laboratory of Forest Genetics, Aristotle University of Thessaloniki

Address:

PO BOX 238 - 54006 Thessaloniki

Tel.:

+30 31 996745

Fax.:

+30 31 200392

Email:

aravanop@for.auth.gr



Research project



Title:

CASCADE (Securing gene conservation, adaptive, breeding potential and utilization of a model multipurpose tree species (Castanea sativa Mill.) in a dynamic environment)

Project leader:

ARAVANOPOULOS Philippos

Participants:

MOULALIS D.

Starting date:

January 2000

Duration:

Three and a half years

Funding institution:

EU

Project objectives

Study of genetic diversity and gene flow among orchards, coppice stands and old-growth natural forests, establishment of progeny/provenance tests, study of adaptive traits.

Sender Institution:

National Agricultural Research Foundation, Forest Research Institute

Address:

570 06 Vassilika, Thessaliniki

Tel.:

+30 31 461171

Fax.:

+30 31 461341

Email:

diamandi@fri.gr



Research project



Project leader:

DIAMANDIS

Starting date:

1996

Duration:

Three years

Funding institution:

General Secretariat of Research and Technology and Greek Forest Service

Italy

Sender Institution:

Dipartimento di Arboricoltura e Difesa delle Piante, Facoltà di Agraria Università di Perugia

Address:

Via Borgo XX Giugno, 74 - 06121 Perugia

Tel.:

+39 75 5856249 - 5856248

Fax.:

+39 75 5856255

Email:

arboree@unipg.it



Research project



Title:

Study of some physiological factors in Castanea sativa Mill. and hybrids

Work Project:

Marcatori genetici in frutticoltura (MARCFRUT)

Project leader:

AGABBIO Mario (C.N.R. Sassari), ANTOGNOZZI Evasio, CARTECHINI Alvaro

Participants:

PROIETTI Primo, FAMIANI Franco, PALLIOTTI Alberto

Starting date:

1996

Duration:

Three years

Funding institution:

C.N.R.

Project objectives

The aim of the experiment was to study some physiological factors on chestnut in order to determine the main factors which interfere in plant productivity and the differences between Castanea sativa and the hybrid Castanea crenata x Castanea sativa.

Project summary and results

All these adaptations allow the chestnut to use the limited radiant energy availability in shaded canopy parts with the maximum benefit for both dry matter production and allocation and to obtain a certain productivity, even if lower than the genetic potentiality of the species. Therefore, with exposition of the orchard, tree density, training system and pruning, it is important to ensure that most of the leaves, above all during their development, receive approximately more than 600-700 µmol photon m-2s-1 for the greater part of the day.

Related publications (D.A.D.P. - Perugia Unit)

Proietti, P., Antognozzi, E., Ferradini, N. & Palliotti, A. 1996 Paclobutrazol effects on Castanea sativa Mill. cultivated in pot.Proceedings of the 3rd Scientific meeting S.O.I., Erice, 10-14/3/96: 549-550. (In Italian).

Frenguelli, G., Ferranti, F., Andreutti, R., Tedeschini, E. & Antognozzi, E. 1997. Anatomy of Castanea spp. leaves in relation with photosynthic activity Ann. Fac. Agr. Univ. Perugia 49: 157-163. (In Italian).

Proietti, P., Palliotti, A., Antognozzi, E. & Ferranti, F. 1997. Patterns of anatomy differentiation, growth and physiological activity following paclobutrazol application in chestnut. Proceedings of the 8th International symposium on plant bioregulators in fruit production. Valencia, Spain, 1-4 April 1997: 177-184.

Antognozzi, E., Famiani, F., Micheli, M., Palliotti, A., Piccioni, E., Proietti, P. & Standardi, A. 1997. Genotype evaluation, propagation, vegetative control and some physiological aspects in chestnut. Proceedings of the Workshop on "Tree physiology and genetic resources of chestnut", Torre Pellice (TO), Italy, 18-21 June 1997. (In press).

Palliotti, A., Proietti, P., Famiani, F., Ferranti, F., Antognozzi, E., Frenguelli, G. & Andreutti, R. 1998. Adaptation mechanisms induced by shadow stress in Castanea sativa Mill leaves. Proceedings of the "National meeting on chestnut". Cison di Valmarino, Treviso, Italy, 23-25 October, 1997: 377-389. (In Italian).

Proietti, P., Palliotti, A., Famiani, F., Antognozzi, E., Ferranti, F., Andreutti, R. & Frenguelli, G. 1999. Influence of leaf position, fruit and light availability on photosynthesis of two chestnut genotype. Scientia Horticulturae in press.

Famiani, F., Proietti, P., Palliotti, A., Ferranti, F. & Antognozzi, E. 1998. Effect of leaf to fruit ratios on fruit growth in chestnut. Proceedings of the Second international symposium on chestnut, Bordeaux (France), 19-24 October 1998.

Sender Institution:

Istituto Sperimentale per la Selvicoltura, Università di Arezzo

Address:

Viale Santa Margherita 80 - 52 100 Arezzo



Research project



Title:

Integrated study of factors involved in degraded chestnut forests in central and Mediterranean Europe. Biological criteria for sustainable forest management

Project leader:

AMORINI Emilio

Participants:

Istituto per la Patologia degli Alberi Forestali, Florence, Italy; Slovenian Forestry Institute, Ljubljana, Slovenia; Plant Health and Soil Conservation Station, Budapest, Hungary; Centre d'Ecologie fonctionelle et evolutive, Montpellier, France; Institute of Forest Ecology, Nitra, Slovakia.

Starting date:

1 Febraury 1999

Duration:

Three years

Funding institution:

The European Community, INCO-COPERNICUS

Objectives and summary of the proposed activity

Sweet chestnut forests in Slovenia were seriously degraded since the introduction of chestnut blight. Besides this disease, other negative biotic factors (mainly ink disease) and negative forest management practices (negative attitude towards sweet chestnut as it was thought to become extinct because of chestnut blight) resulted in the reduction of forest areas with sweet chestnut and its degradation to mostly understroy, coppice tree. After the natural appearance of the hypovirulence of chestnut blight, which is of viral origin, transmissible and cause healing and reduction of virulence of the pathogen, reevaluation of sanitation practices against chestnut blight is needed and there is also a great opportunity to change management and silvicultural practices to promote sweet chestnut in forests where it was once already one of the most important tree species.

In the proposed three year research project the viral nature and other important biological properties of hypovirulence will be defined in Slovenia, where sweet chestnut appears individually or in groups on 22 percent of forested area.

The work will be oriented to fulfill four main goals:

a. Establishment of the real state of sweet chestnut in Slovenian forests:

- analyses of the sanitary condition of sweet chestnut and of the stands where it appears respectively;

- historical survey of the sweet chestnut canker progress (spatial and time analyses);

- analyses of the management, tending and phytosanitary measures.

b. Analyses of the factors influencing sweet chestnut condition:

- inter and intra specific influences, stand construction, biotic and abiotic influences all on stand level and on the level of all forests in Slovenia.

c. Phytosanitary aspects of sweet chestnut:

d. Elaboration of strategies for suitable modifications of management of sweet chestnut forests.

The majority of work will be oriented in the research of the biological basis of chestnut blight hypovirulence and its use. Hypovirulence will be proven by biochemical methods, ds RNA will be characterized, number of compatibility groups of the pathogen in Slovenia will be revealed, pathogenicity of hypovirulent strains will be determined. Methods of inoculation of hypovirulent strains of Cryphonectria parasitica in the bark of sweet chestnut will be tested and modified for the successful spread of hypovirulence. Phytophthora cambivora will be isolated and identified. Methods of management and silvicultural practices in forests with infected sweet chestnut stands will be evaluated. Methods of artificial spread of hypovirulence will be tested for biological control of chestnut blight. Measures of biological control will be compared to the success of silvicultural methods based on management of the chestnut stands where hypovirulence is present.

Sender Institution:

Istituto Selvicoltura - Università degli Studi di Firenze

Adress:

Via San Bonaventura, 13 - 50144 Firenze I

Tel.:

+39 055 461071

Fax.:

+39 055 486604

Email:

ist.selv@cesit1.unifi.it



Research project



Title:

Chestnut orchards restoration

Project leader:

GIANNINI Raffaello

Participants:

TANI Andrea, PROIETTI PLACIDI Anna Maria

Starting date:

1978

Duration:

In progress

Funding institution:

UNIFI, Regional Grant

Project objectives

To define the most useful restoration techniques.

Project summary and results

In different Italian regions (north and south) various experiment areas were identified to define the most effective cultivation practices to restore and improve the surrendered chestnut orchards.

The control of natural vegetation and the pruning of the higher side of the crown inducing a rejuvenating reaction, resulted in more effective practices.

Related publications

Bassi, S., Giannini, R., Lambardi, M. & Tani, A. 1986. First results on the experiences of the pruning effects on abandoned chestnut orchards. Proceedings of the meeting on "Giornate di Studio sul Castagno", Caprarola (VT), Italy. (In Italian).

Giannini, R. 1993. Conservation and improvement of the traditional chestnut orchards. International Congress on Chestnut. Spoleto, Italy.

Giannini, R. & Proietti Placidi, A.M. 1997. Chestnut orchards Cultural and plantation techniques and main grafting methods. Comunità Montana delle Prealpi Trevigiane, TV, 1997. (In Italian).

Giannini, R. & Tani, A. 1992. Limits to chestnut orchard and techniques for the recovery of abandoned chestnut orchard. Proceedings of the meeting on Recovery and valorisation of chestnut orchard. 31 October 1992. Valdobbiadene (TV), Italy. (In Italian).

Lambardi, M. & Tani, A. 1990. Chestnut orchard: considerations on productive recovery of old chestnut orchards, copse conversion and realisation of new orchards. Proceedings of a meeting on Chestnut orchards in Barbagia Mandrolisai. Aritzo, Italy 29 October 1988. (In Italian).

Magini, M., Tani, A. & Canciani, L. 1988. First results of experiments on chestnut (Castanea sativa Mill.) held in Romagna. "Papers in honour of Prof. Alessandro de Philippis", Tip. Coppini, Florence. (In Italian).

Magini, E., Paci, M. & Tani, A. 1982. Notes on chestnut orchards improvement for fruit production. Proceedings of a meeting on Chestnut orchard. Grafting techniques and biological control of cortical canker. Barga (LU), Italy, 15-16 April 1982 E.T.S.A.F. (In Italian).

Maltoni, A., Paci, M. & Tani, A. 1997. Structural and floristic modification in chestnut orchards, following abandon. Proceedings of a National congress on chestnut. Vittorio Veneto (TV), Italy, October 1997. (In Italian).

Maltoni, A., Papi, M. & Tani, A. 1997. Experiences on the use of cultivars of Castanea sativa Mill. for wood production in Lucca province. Proceedings of a National congress on chestnut. Vittorio Veneto (TV), Italy October 1997. (In Italian).

Paci, M. & Tani, A. 1982. Further results on chestnut in Chianti, Italy. L'Italia Forestale e Montana, XXXVII, 4. (In Italian).

Tani, A. & Canciani, L. 1993. Productive recovery of chestnut orchard. Azienda Regionale delle Foreste dell'Emilia Romagna. (In Italian).

Sender Institution:

Istituto Selvicoltura - Università degli Studi di Firenze

Adress:

Via San Bonaventura, 13 - 50144 Firenze I

Tel.:

+39 055 461071

Fax.:

+39 055 486604

Email:

ist.selv@cesit1.unifi.it



Research project



Title:

Estimate genotype/environment interaction of chestnut fruit traits

Project leader:

GIANNINI Raffaello

Participants:

TANI Andrea, PROIETTI PLACIDI Anna Maria

Starting date:

1982

Duration:

In progress

Funding institution:

Regional financial contribution

Project objectives

Nut traits genotype-environment interaction estimate.

Project summary and results

Chestnut cultivars have been introduced in different areas of Central and North Italy. Different traits of nuts have been considered as the genotype-environment interaction. Some cultivars are well adapted in new areas.

Related publications

Berardi, C., Giannini, R. & Tani, A. 1993. Comparison between some Italian Chestnut (Castanea sativa Mill.) cultivars. International Congress on chestnut. Spoleto, Italy.

Borghetti, M., Menozzi, P., Vendramin, G.G. & Giannini, R. 1986. Morphological variation in chestnut fruits (Castanea sativa Mill.) in Tuscany (Italy). Sylvae Genetica 35.

Giannini, R., Rossi, P. & Vendramin, G.G. 1993. Allozyme variation within and among "Marrone fiorentino" cultivated varieties. International Congress on Chestnut. Spoleto, Italy

Tani, A. Tani, A. 1985. First observations on varietal differences in chestnut. Technical Bulletin "Meeting on chestnut orchard ". S. Piero in Bagno (FO), Italy. (In Italian).

Tani, A. 1986. Results on varietal comparison in chestnut. Proceedings of a meeting on chestnut. Caprarola (VT), Italy. (In Italian).

Sender Institution:

Istituto Selvicoltura - Università degli Studi di Firenze

Adress:

Via San Bonaventura, 13 - 50144 Firenze I

Tel.:

+39 055 461071

Fax.:

+39 055 486604

Email:

ist.selv@cesit1.unifi.it



Research project



Title:

Different grafting methods on chestnut

Project leader:

GIANNINI Raffaello

Participants:

TANI Andrea & PROIETTI PLACIDI Anna Maria

Starting date:

1986

Duration:

In progress

Funding institution:

Regional fund A.R.S.I.A. (FI)

Project objectives

To define the most useful grafting methods according to different root-stocks.

Project summary and results

A comparison among different grafting methods was carried out and the most useful one to apply on chestnut was identified. Experiments have been carried out in nursery and in field. The most interesting results are: cleft graft and chip budding seem to be the best methods for seedlings; crown graft appears the most useful for coppice sprouts of 4-5 cm in diameter.

Related publications

Magini, E., Tani, A. & Canciani, L. 1988. First results of experiments on chestnut (Castanea sativa Mill.) held in Romagna. Papers on silviculture in honour of Alessandro de Philippis, Florence. (In Italian).

Giannini, R. & Proietti Placidi, A.M. 1997. Chestnut orchards Cultural and plantation techniques and main grafting methods. Comunità Montana delle Prealpi Trevigiane, TV, 1997. (In Italian).

Maltoni, A., Papi, M. & Tani, A. 1997. Experiences on the use of cultivars of Castanea sativa Mill. for wood production., in Lucca province. Proceedings of a National congress on chestnut., Cison di Valmarino (TV), Italy. (In Italian).

Tani, A. & Canciani, L. 1993. Productive recovery of chestnut orchards.. Azienda Regionale delle Foreste dell'Emilia- Romagna. (In Italian).

Sender Institution:

Istituto di Miglioramento Genetico Piante Forestali - Consiglio Nazionale delle Ricerche

Adress:

Via Atto Vannucci, 13 - 50134 Firenze

Tel.:

+39 055 461071 - 461453

Fax.:

+39 055 468804

Email:

giannini@imgpf.fi.cnr.it



Research project



Title:

Genetic relationships in chestnut genotypes by molecular markers

Project leader:

GIANNINI Raffaello

Participants:

PAFFETTI Donatella, VETTORI Cristina

Starting date:

1996

Duration:

In progress

Funding institution:

C.N.R.

Project objectives

1. Genetic variability among chestnut genotypes called with the same name by the local population (synonymy).

2. Genetic variability among cultivars used for flour, fresh consumption or wood.

Project summary and results

Each analysed genotype is identified by RAPD markers. Moreover, genotypes of different geographic places and called with the same name by the local population show the identical fingerprinting indicating that they are the same cultivar. The genetic relationships are represented in a dendrogram obtained with RAPD markers.

Related publications

Paffetti, D., Giannini, R., Bellini, E. & Giordani, E. 1997. Genetic identification of cultivars of chestnut (Castanea sativa Mill.) by molecular markers. Workshop on tree physiology and genetic resources of chestnut.

Paffetti, D., Bellini, E., Benelli, C. & Giannini, R. 1997. Genetic identification of cultivars of chestnut (Castanea sativa Mill.) by molecular markers. Proceedings of a National congress on chestnutCison di Valmarino, (TR), Italy, (In Italian).

Bellini, E., Giannini, R., Giordani, E. & Paffetti, D. 1997. Genetic identification of cultivars of chestnut (Castanea sativa Mill.) by molecular markers. Proceedings of the "Tree Physiology and Genetic Resources of Chestnut". (In press).

Sender Institution:

Dipartimento di Agrochimica e Agrobiologia - Università di Reggio Calabria

Adress:

P.za S. Francesco di Sales, 2 - 89061 Gallina (RC)

Tel.:

+39 0965 682754

Fax.:

+39 0965 682755

Email:

gmagnano@csiins.unirc.it


ampennisi@csiins.unirc.it



Research project



Title:

Biological control against Cryphonectria parasitica

Project leader:

MAGNANO DI SAN LIO Gaetano

Participants:

PENNISI Alfio Maria, ABENAVOLI Maria Rosa, AGOSTEO Giovanni Enrico

Starting date:

1992

Duration:

Five years

Funding institution:

Calabria Region

Project objectives

Project summary and results

An intensive sampling in the Calabria district confirmed that vc (virulent type) diversity is low. Only four vc types were found among all isolates (271) of C. parasitica in Calabria. Therefore, biological control of chestnut blight might be successfully applied in the orchard in southern Italy.

C. parasitica produces in vitro phytotoxic substances. Culture filtrates of C. parasitica were applied at various concentrations to three different vegetal tissues: 1) calli cultures obtained from leaf explanta of C. sativa, Nicotiana plumbaginifolia and Petunia hybrida; 2) seeds of Triticum durum "Simeto"; and 3) stem cuttings of Lycopersicon esculentum "Marmande". Culture filtrates of C. parasitica significantly reduce the growth of calli and inhibited root and epicotyl extending of treated seeds. The esterase and peroxidase electrophoretic profiles of callus extracts showed modifications due to the treatments with culture filtrates. Modifications of isozyme patterns due to the filtrate treatments were also observed for root and epicotyl extracts. No correlation was found between virulence and phytotoxicity, while electrolyte leakage from tomato leaf explanta increased with isolate virulence.

Results confirm that C. parasitica produces phytotoxic substances that probably play a different role in the host-pathogen relations and might be utilized for in vitro selection of blight resistant chestnut plants.

Related publications

Pennisi, A.M., Parisi, A., Cacciola, S.O. & Magnano Di San Lio, G. 1991. Electrophoretic profiles of normal and hypovirulent strains of Cryphonectria parasitica. Petria, 1: 62-72.

Pennisi, A.M., Granata, G. & Sidoti, A. 1991. Diffusion and mycological aspects of Cryphonectria parasitica in Calabria and Sicily. Mic. Ital., 3:21-26.

Pennisi, A.M., Magnano Di San Lio, G. & Grasso, S. 1992. Vegetative compatibility of isolates of Cryphonectria parasitica (Murr.) Barr. Isolated in chestnut orchards in Calabria region. Petria, 2,1 - 10. (In Italian).

Granata, G., Sidoti, A., Gullotto, A. & Pennisi, A.M. 1992. Incidence of the chestnut bark canker in Sicily and tests of vegetative compatibility. Tecnica Agricola 44 (1):3-10. (In Italian).

Pennisi, A.M. & Minervini, G.M. 1993. Pathogenicity and electrophoretic characterization of Cryphonectria parasitica strains. Proc. Int. Cong. on Chestnut, Spoleto, 20-23 ottobre 1993, pp. 583-586.

Pennisi, A.M. & Agosteo, G.E. 1995. Phytopathological problems of forest ecosystems Calabria region. Proceedings of a meeting on Italian arks and protected areas in the European outlook. S. Stefano d'Aspromonte (Gambarie) (RC), Italy. 8-10 June 1995. (Accepted for publication), (In Italian).

Pennisi, A.M. & Abenavoli, M.R. 1996. Phytotoxic activity of metabolites produced by Cryphonectria parasitica. 9th meeting on Molecular and physiological aspects of the interaction plant-pathogen. Ragusa, 31 May- 1 June. (In Italian).

Abenavoli, M.R. & Pennisi, A.M. 1997. The effect of PVP on chestnut callus formation. Proceedings of a meeting on Plant biotecnology as a tool for the exploation of mountain lands. Turin, 25-27 May 1997. Under publication by I.S.H.S. (In Italian).

Pennisi, A.M. & Abenavoli, M.R. 1997. Phytotoxicity of culture filtrates from Cryphonectria parasitica the causal agent of chestnut blight. Proceedings of a meeting on Plant biotecnology as a tool for the exploitation of mountain lands. Turin, 25-27 May 1997. In press by I.S.H.S. (In Italian).

Pennisi, A.M. & Agosteo, G.E. 1997. Population variability of Cryphonectria parasitica in Calabria region. Proceedings of the 3rd national meeting on Biodiversity: Technologies-Quality. Gallina (Reggio Calabria), Italy. 16-17 June 1997. (In press). (In Italian).

Sender Institution:

Dipartimento di agronomia e produzione vegetale, agropolis

Address:

Via Romea 16 - 35020 Legnaro (PD)

Email:

gponchia@uxl.unipd.it



Research project



Title:

Individuation and morphologic description of Veneto chestnut biotypes

Project leader:

PONCHIA Giorgio

Korea

Sender Institution:

Korean Forest Research Institute

Address:

P.O. Box24, Suwon, Kyonggido 441-350 Korea

Tel.:

+82/331/290/1193

fax:

+82/331/292/8468



Research project



Title:

Improvement of Chestnut Varieties and Management of Chestnut Orchards

Project leader:

CHOI, W.-Y.

Participants:

HWANG, M.-S., AHN, C.-Y., PARK, J.-D., SIN, S.-C., KIM, C.-S, KIM, J.-K., LEE, S.-H., LEE, J.-K., CHOI, K.-S., KU, K.-S., H.-S. J.

Starting date:

January 2000

Duration:

Ten years

Funding institution:

Government

Total cost (US$):

108 000

Project objectives

1. Breeding of new chestnut varieties.

2. Improvement of chestnut cultivation techniques and methods.

3. Improvement of system in chestnut pest and disease control.

4. Prediction of chestnut yields.

Project summary and results

1. Breeding of new chestnut varieties:

New chestnut varieties producing high nut yields and larger nuts and being resistant to pest and disease and adaptable to severe climate condition.

Conservation of useful and rare chestnut germplasms.

2. Improvement of chestnut cultivation techniques and methods:

Selection of useful local cultivars (or strain);

Regeneration (rejuvenation of tree vigour) of older trees by strong prunning and training;

Soil survey and fertilization trials in chestnut orchards.

3. Improvement of system in chestnut pest and disease control:

Disease: control of canker disease;

Pest: control of chestnut gall wasp, peach pyralid moth and chestnut curculio;

Others: air control by chopper, selection of insecticides and ecological change of soil micro animals

4. Prediction of chestnut yields:

Chestnut yields per tree and standardization of chestnut yield table.

Related publications

Hwang, M.-S. 1999. Introduction of new chestnut varieties. F.R.I., For. Inform. No.102: 31-33.

Hwang, M.-S. & Ahn, C.-Y. 1998. Nut characteristics of hybrids among the early nut-ripening chestnut varieties. Proc. of second Int. chestnut symp. held in (Bordeaux) France, 19-23 October 1998.

Sin, S.-C. et al. 1998. Development of attractants for chestnut weevils, Curculio spp. K.F.S., F.R.I. pp.1-81.

Lee, S.-H., Lee, J.-K., Hwang, M.-S. & Moon, B.-J. 1999. Effect of biological and environmental factors on the occurrence of chestnut cankers in chestnut forest stands. In "Proceedings of Kor. Plant Pathol. Soc." held in (Andong) Korea, 29-30 October 1999.

Netherlands

Sender Institution:

Institute for Forestry and Nature Research (IBN-DLO)

Address:

P.O.Box 23 - 6700 AA Wageningen

Tel.:

+31 317 477700

Fax.:

+31 317 419000

Email:

a.oosterbaan@alterra.wag.ur.nl



Research project



Title:

Growing quality timber on arable land, including chestnut

Project leader:

OOSTERBAAN A.

Starting date:

1995

Funding institution:

IBN-DLO

Project objectives

The aim of the research is to examine the production of quality timber (heartwood) of some species including chestnut, on former (fallow or abandoned) arable land and to observe which growing system (plant distance, maintenance) is most effective.

Project summary and results

The main occurence of Castanea sativa in the Netherlands is as a solitary tree and as mixture species in a few hundred hectares of forest. An inventory of growth and site characteristics shows promising timber production possibilities; on good sites (former arable land), diameters of 30-40 cm can be achieved at an age of 30-40 years. There are no serious problems with diseases. The problems of ring shake should be solved by a good way of tending.

Related publications

Oosterbaan, A. & Peeters, J.P. 1997. Genetic requirements for Chestnut in the Netherlands.

Peeters, J.P., van den Berg, C.A. & Oosterbaan, A. 1995. Growth of Castanea sativa in The Netherlands (in Dutch). Report 197. IBN-DLO, Wageningen. 23 p.

Sender Institution:

Institute for Forestry and Nature Research (IBN-DLO)

Address:

P.O.Box 23 - 6700 AA Wageningen

Tel.:

+31 317 477700

Fax.:

+31 317 419000

Email:

a.oosterbaan@alterra.wag.ur.nl



Research project



Title:

Urban Green. Water use and drought tolerance of (urban) trees

Project leader:

KOPINGA Jitze

Starting date:

1994

Duration:

Five years

Funding institution:

BN-DLO

Project objectives

Studies of the water use of various urban and landscape tree species and cultivars within the species to determine their adaptability for drought sensitive sites.

Project summary and results

Studies were performed in which the absolute and relative water use during the growing season and also the daily pattern of water is determined by gravimetric methods. Species that have been regarded so far are: Quercus suber, Castanea sativa, Acer pseudoplatanus, Tilia x vulgaris, Acer pseudoplatanus. In a later stage also Ginkgo biloba, Pinus sylvestris, Picea abies, Larix kaempferi, Aesculus hippocastanum, Ulmus x hollandica and Robinia pseudoacacia are taken into consideration. Peliminary results already have been monitored but not yet officially published.

Related publications

Kopinga, J. 1991. The effects of restricted volumes of soil on the growth and development of street trees. Journal of Arboriculture 17 (3): 57-63.

Kopinga, J. 1995. Water use and water supply of urban trees. Groen 51 (3): 22-27.

Portugal

Sender Institution:

Direcção Regional de Agricultura de Trás-os-Montes (DRATM)

Address:

Quintado Valongo 5370 Mirandela

Tel.:

+351 278 260 900

Fax.:

+351 278 260 954

Email:

olga.borges@dratm.min-agricultura.pt



Research project



Title:

Contribution to the development of the chestnut culture (Castanea sativa Mill). Photosynthetic, morphometric, isoenzimatic and nutritional characterization of cultivars

Project leader:

Universidade de Trás-os-Montes e Alto Douro

Participants:

DRATM, UTAD

Starting date:

January 1997

Duration:

Four years

Funding institution:

Community programme

Project objectives

Prospection of cultivars in the Trás-os-Montes region and the morphometric, isoenzimatic and nutritional characterization, constitute a cultivar collection, constitution of trial with the aim to determine the mineral nutrients necessary for the good development of chestnut trees.

Sender Institution:

Universidade de Trás-os-Montes e Alto Douro

Address:

Qta. De Prados - 5000 Vila Real

Tel.:

+351 259 350508

Fax.:

+351 259 350480

Email:

cgabreu@utad.pt



Research Project



Title:

Chestnut ink disease. an integrated approach to its control

Project leader:

GOMES ABREU Carlos

Participants:

KOPINGA Jitze, GUEDES PINTO H., PIRES A. Luisa, LOPES GOMES A., TORRES CASTRO L.

Starting date:

1993

Duration:

Five years

Funding institution:

NATO/Science for Stability Programme III, PDRITM II, UTAD

Project objectives

To obtain resistant chestnut rootstocks within the European species and a set of management practices in order to create conditions less favourable for the ink pathogen and more favourable for the trees.

Project summary and results

Take advantage from durable resistance observed in the field for decades encompassing resistance to subsequent colonization by Phytophthora cinnamomi following successful infection; taking advantage from the occurrence of situations where susceptible chestnut are not diseased in the presence of pathogen. This calls for an accurate survey in the area in order to recognize supposed suppressive soils to ink disease.

Sender Institution:

Universidade de Trás-os-montes e Alto Douro (UTAD)

Address:

Apert. 202 - 5000 Vila Real

Tel.:

+351 259 321631

Fax.:

+351 259 320480

Email:

alpires@utad.pt



Research Project



Title:

Interactions forest-environment in ecosystems subject to be disturbed by natural and antropic factors in regions between the Atlantic and Mediterranean

Project leader:

PIRES Ana Luisa

Participants:

PORTELA Ester, COELHO Celeste, FERREIRA Antonio & PEREIRA Bruno (University of Aveiro)

Sender Institution:

Direcção regional de agricultura da Beira interior

Address:

Rua Amato Lusitano Lote 3 - 6000 Castelo Branco



Research project



Title:

Selection, preservation and study of Portuguese chestnut varieties

Project leader:

SANCHES PEREIRA Josè

Starting date:

1990

Project objectives

The study of the agronomic behaviour of some traditional chestnut varietes in order to select the most interesting material for the new chestnut plantations.

Sender Institution:

Estação Florestal Nacional

Address:

Rua do Borja, 2 - 1350 Lisboa

Tel.:

+351 1 3901661

Fax.:

+351 1 3974268

Email:

nop11387@mail.telepac.pt



Research project



Title:

Conservation and Breeding of Genetic Resources of European Chestnut (Castanea sativa Mill)

Project leader:

ALBUQUERQUE SARDINHA Raul

Participants:

COSTA SEABRA Rita, MACHADO M Helena (Estação Florestal Nacional)

PAIS M Salomé (Fundação da Faculdade de Ciências de Lisboa)

LORETO MONTEIRO Maria, MARTINS Anabela (Escola Superior Agrária de Bragança)

Castanea (Private Institution)

Starting date:

January 1997

Duration:

Three years

Funding institution:

Fundação Ciência e Tecnologia - Praxis XXI Programme

Project objectives

Identification of progression areas of ink disease in chestnut stands

Project summary and results

Areas of ink disease were identified in chestnut stands. Transgenic plants of European Chestnut were obtained integrating uidA and b1,3 glucanases. The molecular characterization by RAPD enabled the identification of ink disease resistant clones. The micropropagation and mycorrhization of selected clones was also established with success.

Related publications

Seabra, R.C. & Pais, M.S. 1998. Genetic Transformation of European Chestnut (Castanea sativa Mill.) Plant Cell Rep. 17:177-182.

Seabra, R.C. & Pais, M.S. 1998. Molecular separation of Castanea species and Hybrids and Analysis of Variation of Chestnut Stands in Portugal. Proceedings of Cost G4 - Multidisciplinary Chestnut ResourcesR WG 2 - Genetic Resources. Santiago de Compostela, España. 41-43.

Seabra, R.C. & Pais, M.S. 1998. Genetic Transformation of Castanea sativa with genes of interest. Acta Horticulturae (submitted).

Seabra, R.C., Serrazina, S., Cotrim, H. & Pais, M.S. 1999. Molecular characterisation of Portuguese chestnut clones by RAPD. Plant Cell Reports (submitted).

Slovak Republic

Sender Institution:

Institute of Forest Ecology, Slovak Academy of Sciences, Zvolen, Branch of Woody Plants Biology

Address:

Akademická 2 - 949 01 Nitra

Tel.:

+421 87 35738 - 418381

Fax.:

+421 87 35696

Email:

nruejuha@savba.savba.sk



Research project



Title:

Protection of European Chestnut against Cryphonectria parasitica (Murr.) Barr in Slovakia

Project leader:

JUHÁSOVÁ Gabriela

Participants:

HRUBÍK Pavol, LEONTOVYC Roman (Forestry Research Institute, Zvolen)

Starting date:

January 1991

Duration:

Six years

Funding institution:

Slovak Ministry of Agriculture

Total cost (US$):

16 700

Project objectives

Project summary and results

Incidence of chestnut blight was recorded in 67 orchards distributed in 18 localities within the Slovak Republic. All isolates of Cryphonectria parasitica were virulent and no hypovirulent strain was found. Four hypovirulent strains imported from France were proved effective and 3 586 canker wounds were treated with them in form of pellets manufactured for this purpose by the Slovak company Fytofarma, Bratislava. Effectiveness of treatment assessed by the healing of canker wounds ranged from 28-82 percent.

Related publications

Juhásová, G. & Berthelay-Sauret, S. 1993. Health condition of Castanea sativa Mill., incidence of the fungus Cryphonectria parasitica (Murr.) Barr in Slovakia. Biotechnológia, 1, 20, 55-58 p.

Juhásová, G. 1993. The biology and distribution of the fungus Cryphonectria parasitica (Murr) Barr in Slovakia. In: Proc. from the Intern. Conference on Ecological Stability, Diversity and Productivity of Forest Ecosystems, Zvolen, 125 p.

Juhásová, G. & Radócz, L. 1995. Biological control of chestnut blight (Cryphonectria parasitica /Murr./ Barr) with the hzpovirulent strains. Novényvédelem 31, 169-176 p.

Juhásová, G. 1995. Application of biological control with hypovirulence, liquidation of centres of infection by the fungus Cryphonectria parasitica (Murr.) Barr in Slovakia. Final report for the Ministry of Agriculture, Bratislava, 86 pp.

Sender Institution:

Institute of Forest Ecology, Slovak Academy of Sciences, Zvolen, Branch of Woody Plants Biology

Address:

Akademická 2, 949 01 Nitra (Slovak Republic)

Tel.:

+421 87 35738 - 418381

Fax.:

+421 87 35696

Email:

aurelia@sav.savzv.sk



Research project



Title:

Propagation of endangered, rare and introduced woody plants using in vitro methods

Project leader:

KAMENICKA Aurelia

Participants:

Number of co-workers (eight)

Starting date:

January 1994

Duration:

Three years

Funding institution:

Slovak Academy of Sciences

Project objectives

Publications

Kamenicka, A. 1994. Micropropagation of Magnolia x soulangiana Soul.-Bod. after a long-term culture in vitro. In: Facsar G. (Eds), A Kertészeti és Élelmiszeripari Egytem Közleményei. Budapest, 129-132.

Kamenicka, A. 1994. Rapid micropropagation of Magnolia x soulangiana Soul.-Bod. from mature trees. In: International Plant Propagators Society in Bulgaria, 30 October-1 November 1994. Sofia, 31-35.

Kamenicka, A. & Valová, M. 1994. The effects of culture media on formation of axillary shoots of Magnolia x soulangiana Soul.-Bod, in vitro. Annali di Botanica (Italy) 12, 37-43.

Kamenicka, A. 1995. Micropropagation of Magnolia Species. Conference on Plant In vitro Culture, Mosonmagyaróvár, 1-3 September 1995, Hungary, 9 p.

Kamenicka, A. 1996. Environmental control formation of axillary shoots Magnolia x soulangiana Soul.-Bod. in vitro. IPP Society, 17-18 October 1996, Seek and Share, Budapest, 36-37.

Kamenicka, A. 1996. Rooting of Magnolia x soulangiana microcuttings. Biológia 51, 435-439.

Kamenicka, A. 1996. Micropropagation of saucer magnolia (Magnolia x soulangiana Soul.-Bod.) from mature trees. IPP Society, 17-18 October 1996, Seek and Share, Budapest, 31-35.

Kamenicka, A. 1996. Formation of axillary shoots of Castanea sativa Mill. in vitro. Annali di Botanica, 54, 183-187.

Kamenicka, A. & Lanakova, M. 1996. Rapid micropropagation of Magnolia species. Biológia 51, 82-83.

Kamenicka, A. & Lanakova, M. 1996. Axillary shoot regeneration and plant production from ex-plants of Magnolia x soulangiana Soul.-Bod. Folia dendrologica 20-21, 297-303.

Kamenicka, A. & Lanakova, M. 1996. Stomata of saucer magnolia (Magnolia x soulangiana) leaves cultured in vitro. Biológia 51, 42 p.

Kamenicka, A. & Valka, J. 1996. Optimization of cytokinin concentrations for regeneration of Rhododendron hybridum. 10th FESPP Congres, 9-13 September 1996. Florence, Italy, 59 p.

Kamenicka, A., Lanakova, M. & Valová, M. 1996. Effects of culture media on the formation of axillary shoots of Magnolia x soulangiana Soul.-Bod. in vitro. Acta Agronomica Hungarica 44, 53-57.

Kamenicka, A., Valka, J. & Tomasko, I. 1996. Magnolias in the landscape. Int. Plant Prop. Soc. "Rozmnozovanie na dekorativni rastenija". 5-7 October 1996, Sofia, 62-66.

Lanakova, M. & Kamenicka, A. 1996. Differences in stomata anatomy of Magnolia x soulangiana Soul.-Bod. Medzinárodná konf. "Rastlina v podmienkach stresu", Nitra 18-19 June 1996, Slov. polnohosp. univerzita, 48-51.

Valova, M., Krajcova, D. & Kamenicka, A. 1996. Changes of mineral elements in explant of Magnolia x soulangiana Soul.-Bod. during the culturing in vitro. Folia dendrologica 20-21, 331-339.

Kamenicka, A. & Takats, J. 1997. Direct regeneration of Magnolia spp. via in vitro propagation. Magnolia 61, 1-6.

Sender Institution:

Institute of Forest Ecology, Slovak Academy of Sciences, Zvolen, Branch of Woody Plants Biology

Address:

Akademická 2 - 949 01 Nitra

Tel.:

+ 421 87 35738 - 418381

Fax.:

+ 421 87 35696

Email:

nruejuha@savba.savba.sk



Research Project



Title:

Rehabilitation and conservation of European Chestnut stands in localities of Slovakia (particular projects for each of 12 localities involved)

Project leader:

JUHASOVA Gabriela

Participants:

BOLVANSKI Milan Representatives of Local Government

Starting date:

1991

Duration:

Seven years

Funding institution:

Ministry of the Environment of Slovakia

Project objectives

Project summary and results

Within project implementation 4 658 trees within the Slovakia Region were investigated. Out of them 1 685 were proved damaged by chestnut blight. Four hundred and fifty-five trees and nearly 10 000 significantly damaged stump sprouts were cut out and on the remaining infected trees, biological control by hypovirulent strains of Cryphonectria parasitica was carried out.

Related publications

Bolvanský, M. & Juhásová G. 1995. European Chestnut in ecological agriculture. In: Proc. of the seminar on "Sustainable management in agricultural habitat", Nitra.

Uhásová, G. Biological protection of European Chestnut against the fungus Cryphonectria parasitica (Murr) Barr in Slovakia. Proc. of the conference on "Forestry and research in the changing ecological conditions in Slovak Republic", Zvolen, 113-118 p.

For other publications see the form of research project: "Protection of European Chestnut against Cryphonectria parasitica in Slovakia".

Slovenia

Sender Institution:

Biotechnical Faculty, Agronomy Deptartment, University of Ljubljana

Address:

Jamnikarjeva 101 - 1111 Ljubljana

Tel.:

+386 62 211755

Fax.:

+386 62 211755



Research project



Title:

Morphologic, pomologic and genetic evaluation of autochthon chestnut genotypes in Slovenia

Project leader:

ŠTAMPAR Franci

Participants:

SOLAR A., PODJAVORŠEK A., ŠIFTAR A., KODRI I., PERIC V., HOEVAR I., MARJETI A., MUHIÈ S., AKŠ J. & PLIBERŠEK T.

Starting date:

1990

Duration:

Twenty years

Funding institution:

Biotechnical Faculty, Branch Office Maribor; KVZ Nova Gorica

Project objectives

In Slovenia, chestnut grows on more than 200 000 hectares. About 10 percent is chestnut forests, while the remaing area is divided between smaller chestnut stands or solitary trees on the borders of the forests or in the meadows. Due to generative multiplication through the centuries, genotypes are very different concerning morphological and pomological characteristics. Evaluation of this variability has been tried using classical pomological and morphological analysis, complemented with isozyme and DNA analysis (RAPDs).

Project summary and results

Some areas under chestnuts are systematically included into observations and a number of trees (approximatelly 7 percent to date) has been analysed. Extreme morphological and pomological variability is ascertained between chestnuts which belong to different regions. These results are in agreement with results of isoenzymatic analysis. They show great electrophoretic polimorphism in esterase. It is possible to distinguish chestnut genotypes from different regions using the electrophoretic pattern of this enzyme. DNA analysis are still in the initial stages. It is espected that they would explain genetic variability of chestnut genotype population better than any other analysis.

Related publications

Peric, V. 1990. Variabilnost pomoloških lastnosti selekcioniranih klonov kostanja (Castanea sativa Mill.) na obmoèju Goriških Brd, Vipavske doline, Krasa in Brkinov. Sad (1)11:11-13.

Podjavoršek, A. 1997. Pomological and isozymic analysis of different types of chestnut (Castanea sativa Mill.). Master of science thesis, Ljubljana, 1997: 84 p.

Podjavoršek, A. et al. 1997. Pomological analysis of different types of chestnut (Castanea sativa Mill.) in Slovenia. Zbornik BF 69:85-90.

Solar, A., Štampar, F., Podjavoršek, A. & Šiftar, A. 1997. Chestnut research in Slovenia. COST G4. Multidisciplinary chestnut research. Workshop on "Tree physiology and genetic resources of chestnut". Torre Pellice (Turin), Italy, 18-21 June 1997:14.

Solar, A. et al. 1998. Pomological variability of the chestnut (Castanea sativa Mill.) in Slovene. COST G4. Multidisciplinary chestnut research. 2nd meeting, Santiago de Compostela, Spain, 14-16 May 1998. Book of summeries: 50,51.

Šibav, D. & Kodri, I. 1993. Inventarizacija marona in kostanja na Primorskem. Sad (4)10:9-12

Spain

Sender institution:

Centro De Investigacións Forestais De Lourizan.

Address:

Carretera De Marin Km 4, Apt. 127 - 36080 Pontevedra

Tel.:

+34 86 856400

Fax:

+34 86 856420

Email:

fina@inia.es



Research project



Title:

Genetic variability of wild populations and grafted cultivars of Castanea sativa Miller

Project leader:

FERNANDEZ LOPEZ Josefa

Participants:

MIRANDA FONTAIÑA Eugenia, BLANCO BLANCO Maria, DIAZ VAZQUEZ Raquel (CIF Lourizan) PEREIRA LORENZO Santiago (Eps Universidad Santiago De Compostela)

Starting date:

January 1999

Duration:

Four years (1999-2003)

Funding institution:

INIA (SC99-036-C2)

Project objectives

Wild populations:

1) Spanish populations provenance test.

2) Open progeny test of nothwestern Castanea sativa Spanish populations.

3) Introgression of Castanea crenata in Castanea sativa Spanish populations.

Grafted varieties:

4) Selection of rootstocks and varieties.

5) Aptitude to conservation of chestnut varieties.

6) Morphological identification of central and south Spanish cultivars.

7) Influence of the pollen on nut size.

Project summary and results

The genetic variability of Castanea sativa wild populations is now being studied with funds from national projects. The actual objectives are:

Related publications

Pereira, S., Ascasibar, J. & Ramos, A. 1999. Spanish scheme in chestnut improvement for nut and timber production. FAO-CIHEAM-Nucis-Newsletter, 8: 32-34.

Pereira-Lorenzo, S., Fernández-López, J., Varela-Arias, I., & Sau, F. 2000. Comportamiento de patrones híbridos de castaño resistentes a la tinta en zonas de castañar. Investigación Agraria. Sistemas y Recursos Forestales, 9(1): 89-101.

Fernandez-López, J., Diaz-Vazquez, R., Cogolludo-Agustin, M.A. & Pereira-Lorenzo, S. 2000. Conservación de recursos genéticos de las frondosas nobles en España. Investigación Agraria. Sistemas y Recursos Forestales. Fuera de Serie, 2: 71-93.

Sender institution:

Centro De Investigacións Forestais De Lourizan.

Address:

Carretera De Marin Km 4, Apt. 127 - 36080 Pontevedra

Tel.:

+34 86 856400

Fax:

+34 86 856420

Email: fina@inia.es




Research project



Title:

Integral development of chestnut in Asturias

Project Leader:

SANCHEZ TAMES Ricardo (Universidad De Oviedo)

Participants:

FERNANDEZ LOPEZ J. (Cif Lourizan), PEREIRA LORENZO Santiago (Universidad De Santiago De Compostela)

Starting Date:

June 1999

Duration:

Three years (1999-2001)

Funding Institution:

Program FEDER (1FD97-0955-CO3-03)

Project Objectives

Applied chestnut research in Asturias

1. Sanitary status of chestnut in Asturias.

2. Selection and conservation of C. sativa grafted varieties.

3. Field clonal tests of hybrids (C. crenata x C.sativa) for forest use in areas with Chryphonectria parasitica. Variability of wild C. sativa in open pollinated progeny test and seed orchard establishment.

4. Biotechnology applied to rootstock production.

Related publications

Pereira-Lorenzo, S. & Fernandez-Lopez, J. 2000. El castaño. En: La Horticultura Española (F. Nuez y G. Llácer, Eds). Sociedad Española de Ciencias Hortícolas, 280-284.

Pereira-Lorenzo, S., Diaz-Hernandez, B., Ciordia-Ara, M., Ascasibar-Errasti, J., Ramos-Cabrere, A.M. & Sau, F. 2001. Spanish chestnut cultivars. Hort. Science, 36(2):344-347.

Turkey

Sender Institution:

Department of Horticulture, Faculty of Agriculture Uludag University

Address:

Gorukle Campus - 16348 Borsa

Tel.:

+90 224 4428078

Fax.:

+90 224 4428077

Email:

userdar@samsun.omu.edu.tr



Research project



Title:

Research on chestnut micropropagation

Project Leader:

SOYLU ARIF

Participants:

ERTÜRK Ümran

Starting date:

1999

Duration:

Three years

Funding institution:

Uludag University, Department of Horticulture, Faculty of Agriculture, Bursa (Turkey)

Total cost (US$):

1 000

Project objectives

To find one cheap, easy and productive method for multiplication of the desired chestnut materials.

Project summary and results

In this study, softwood and hardwood cuttings of some cultivars and rootstocks will be treated with various concentrations of IBA and placed in rooting beds which have bottom-heat and mist arrangements. No results have yet been obtained.


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