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1. Equipment for Capturing and Transporting Brood Fish

  1. Fishing nets (large mesh: 25–40 mm) for catching brood fish; length and depth in proportion to the dimensions of the brood fish pond

  2. Various sizes of scoop nets, open at both ends

  3. Hammocks with removable burlap or net cover (for short distance transportation of brood fish)

  4. Satchels made of a half sack (for transporting individual fish over short distances)

  5. Tanks of 100–500 l capacity, made from iron sheet, aluminium, or fibreglass (to hold the brood fish for anaesthetizing)

  6. Tanks, cisterns, or containers with a continuous oxygen supply facility (for long distance transportation of brood fish)

  7. Scales (for weighing brood fish)

  8. Towels

2. Equipment for Hypophysation

  1. Hypodermic syringes (2, 5, and 10 ml) with sets of needles

  2. Porcelain mortar (5–7 cm diameter) with pestle (for pulverising dry pituitary glands)

  3. 100–200 ml wide-mouthed bottle (for saline solution)

  4. Hand centrifuge, with at least five sets of centrifuge tubes

  5. Electric centrifuge with tubes (if available)

  6. Absolute alcohol, or any other suitable disinfectant (100–200 ml), for disinfecting syringes and needles after use

  7. Threads of different colours cut into 15–20 cm lengths (for marking brood fish)

  8. Darning needles (for marking brood fish)

  9. Needles, strong cotton thread, and pliers (for suturing female breeders)

  10. Small cotton stuffed anaesthetizing bag (for putting inside the breeder's mouth after soaking in MS 222 solution)

  11. Graduated measuring cylinder and wide-necked bottles

  12. 60 × 40 cm size rubber-foam mat and a small foldable table (for holding the fish while injecting)

  13. Towels

  14. Laboratory balance with a set of weights (for weighing chemicals, glands, etc.)

3. Equipment for Stripping and Fertilization

  1. 2–5 l capacity plastic bowls (at least 20)

  2. 10–12 l capacity plastic buckets, preferably graduated (at least 10)

  3. Towels

  4. Milt collectors and/or special pipettes (at least five)

  5. Strong feathers (dry) or plastic spoons (for mixing the sexual products)

  6. Plastic mugs, preferably graduated

  7. Scale (for weighing eggs)

  8. Plastic bottles and straining spoons

  9. Work table and chairs

  10. Scoop nets, open at both ends

  11. Small net (for catching brood fish from the ward tank)

  12. Tanks of 100–200 l capacity (for keeping or anaesthetizing the brood fish)

  13. Scissors, knives, and other tools

  14. Kitchen funnels of different sizes

4. Equipment for Collecting Fertilized Eggs from the Tank

  1. Egg collectors

  2. Mugs, 0.5 l

  3. Plastic bowls (flat)

  4. Plastic buckets, preferably graduated

  5. Rubber tubes (cut sections of motor cycle tube)

  6. Fine meshed (0.4–0.5 mm) scoop nets; large (dia. 60–70 cm) and small (dia. 30–40 cm)

  7. Small net (for catching the brood fish in the ward tank)

5. Equipment for Fish Egg Incubation, Larva Rearing and Fry Nursing

  1. Several incubator devices and larva-rearing devices, with accessories and tools (screw driver, hammer, needle, thread, etc.)

  2. Rubber or plastic tubes of suitable diameter

  3. Plankton net

  4. Petri dishes

  5. Graduated mugs

  6. Plastic buckets

  7. Kitchen funnels

  8. Thermometers (0–50°C)

  9. Magnifying glass (20x), stereo-microscope, or microscope

  10. Dissecting set

  11. Electric hot plate or other heating equipment

  12. Electric kitchen mixer (for preparing feed)

  13. Fine brushes (for cleaning incubators and larva-rearing devices)

  14. Brushes for scrubbing and cleaning basins

  15. Sieve cloth of different meshes (0.3 mm, 0.5 mm, and 0.7 mm) and mosquito netting cloth of mesh sizes 1.0, 1.5, and 2.0 mm

  16. Plastic sheets and linen (for repair and/or fabrication of hatchery devices)

  17. Fine meshed (2–5 mm) nets and fine meshed net boxes (for catching and keeping young fish)

  18. Larger sized (0.5 to 2.0 l) kitchen strainer made of plastic or enamelled metal (for estimating the number of young fish)

6. Equipment for Collecting, Handling and Storing Pituitary Clands

  1. Electric drill with hole-saw of 2.5–3.0 cm diameter, crosscut-saw, or a strong knife (for cutting and removing the top of fish skull)

  2. Wooden support or frame for holding the fish nead while drilling or sawing

  3. Screw driver

  4. Fine pincers (tweezers) or forceps for lifting the pituitary gland

  5. Phials with cork to collect the glands in acetone

  6. A bottle of pure acetone

  7. Cotton

  8. Petri dishes

  9. Desiccator with silica-gel or other water absorbent (for storing acetone-dried pituitary glands)

  10. A bottle of absolute alcohol

7. Equipment for Transporting Young Fish (Just Feeding Fry, Advanced Fry, and Fingerlings)

  1. Plastic bag of 140–160 cm circumference, 65–75 cm high; or plastic bag material having a thickness of 0.3–0.5 mm

  2. Gas reductor (manometer with pressure reducing valve) with thick rubber pipe

  3. Oxygen cylinder

  4. Strong twine for tying the bags

  5. Oxygen gas distributing device

  6. Scoop nets of fine mesh (0.5–1.0 mm), open at both ends

  7. Plastic buckets

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