Prepared at the 53rd JECFA (1999) and published in FNP 52 Add 7 (1999), superseding tentative specifications prepared at the 37th JECFA (1990), published in FNP 52 (1992). | |
SYNONYMS |
Glycogenase |
SOURCES |
Produced by the controlled fermentation of Bacillus subtilis containing the gene for -amylase from Bacillus megaterium. The strain of Bacillus subtilis is non-pathogenic and non-toxicogenic (for example ATCC 39,701). When fermentation is complete, the broth is clarified by centrifugation or filtration. The clarified broth containing the soluble enzyme is ultrafiltered to produce the desired activity. |
ACTIVE PRINCIPLES |
-Amylase |
SYSTEMATIC NAMES AND NUMBERS |
1,4--D-Glucan glucanohydrolase (EC 3.2.1.1) |
REACTIONS CATALYSED |
Endohydrolysis of 1,4--D-glucosidic linkages in polysaccharides, containing three or more 1,4--linked D-glucose units |
SECONDARY ENZYME ACTIVITIES |
Glycosyl transferase, protease |
DESCRIPTION |
Typically tan to dark brown liquid containing the active enzyme |
FUNCTIONAL USES |
Enzyme preparation |
GENERAL SPECIFICATIONS |
Must conform to the General Specifications for Enzyme Preparations Used in Food Processing (FNP 52 & Addendum 6) |
CHARACTERISTICS |
|
IDENTIFICATION |
|
-Amylase activity (FNP 5) |
The sample shows bacterial -amylase activity |