Utilisation of Mulberry in Animal Production Systems

Jorge Benavides

Researcher, CATIE, Turrialba, Costa Rica


I. Forage Trees

Livestock Production and Natural Resources

Numerous traditional land use practices (deforestation, extensive and extractive grazing, lack of erosion control techniques, agriculture in unsuitable zones, etc.) result in alterations to ecological balance and in soil productive capacity (Garríguez, 1983; Jiménez, 1983; Heuveldop and Chang, 1981. Tropical grass yields and quality are affected by climatic factors (Minson and McLeod, 1970; Stobbs, 1975; Cunillos et al, 1975) and by land and capital limitations predominant in most small farms (Ávila et al., 1982).

Apart from the socio-economic aspects, the later is related to the type of agricultural technology historical practised in Central America from the times of the Spanish colonisation. In pre-Colombian times, the great Pleistocene herbivores had disappeared (Jansen and Martin, 1982) and there were no domestic ruminants. There were only autochthonous deer, which are mostly browsers (Sands, 1983; Morales, 1983). The predominant vegetation was composed of shrubs and trees, and apart from maize, there were few members of the gramineae family, without contributing much to feed the autochthonous herbivores. (Jansen and Martin, 1982; UNESCO, 1979; National Geographic, 1992; Skerman and Riveros, 1992). This indicates that the natural vegetation looked very different then compared to now.

Spanish colonial settlement implied the introduction of land use technologies imported from temperate areas, like the plow and the grasses to feed farm animals (Meza and Bonilla, 1990; Tosi Jr. and Voertman, 1977). Those technologies, still in use, have significantly contributed to the loss of natural soil cover and biodiversity. This has also avoided the rational utilisation of forests aiming at questionable productivity in the medium and long terms. In relation to traditional livestock keeping, "... it is little encouragement fact, for the grass pasture experts, to realise that, there are more animals feeding on shrubs and trees, or in associations in which woody plants have a major role, than on true grass and leguminous pastures" (Commonwealth Agricultural Bureau Publication, No. 10, 1974, cited by Skerman et al., 1991).

The establishment of agricultural areas in virgin land has been part of a process, which starts with cereal planting taking advantage of the high soil fertility right after the slash of the forest. Once this fertility declines, land is abandoned or destined to grazing, mostly extensive and extractive (Sands, 1983). Since the fifties, more than 50% of the forests have substituted by migratory agriculture or by grasslands (Collins, 1990; UNESCO, 1979; National Geographic, 1992), which in most cases, are in scattered plots belonging to small farms, or have low carrying capacity in the large farms (Collins, 1990). In Central America, without large amount of inputs and labour, the productivity of grasslands can not be maintained. This is partly due to invasion of autochthonous woody plants, "..while man insists in keeping grassland, natures fights for establishing forests" (Skerman and Riveros, 1992).

The question then arises: What would have happened in the American Tropics, if instead of introducing the plow and the grasses, appropriate technologies aiming at a rational use of trees and shrubs had been developed? Apart from wood, could other products be extracted from forests to satisfy the demand of expendable goods demanded by the population? To provide a partial response to those questions aims the research on forage trees and shrubs, in particular that of mulberry.

The above considerations, added to the lack of access of small and medium size farmers to appropriate production technologies; to the high population growth; and to other aspects related to the socio-economical situation of Central America, indicate the necessity for novel solutions which allow substantial changes to current production practices. In this changing process, the development of technologies more suitable to the ecological and socio-economic conditions of the regions should play a decisive role in the generation of consumable goods in a sustainable manner and with a rational use of natural resources.
 

Trees and Shrubs as Feed for Ruminants

The use of trees and shrubs for ruminant feeding is a known practice among producers in Central America from decades. This empirical knowledge about forage proprieties of various species, is of great value for science. In several studies to characterise production systems, producers report a large number of species for browsing and for cut-and-carry systems with animal in confinement (Ammour y Benavides, 1987; Arias, 1987). The more systematic recognition of the resource is the aim of the research work on forage trees carried out in Central America, part of which is reported in this article.

The studies on the subject have been oriented towards the valuation as a source of forage, of trees and shrubs, and their integration into ruminant production systems (Benavides, 1989). The focus has been on agroforestry and farming systems, and the aim has been to develop technological alternatives allowing more sustainable production and rational use of soil and forest resources.

In order to consider a tree or a shrub as forage, it must have advantages from the points of view of its nutritional value, its yield or its agronomic versatility, above traditional forages. In that sense, the requirements to qualify are: i) nutritional content and intake which will allow animal performance improvements; ii) resistance to repeated pruning/harvesting; iii) high biomass yields per unit of area. Apart from these features, it is advisable to select natives species to take advantage of their adaptation to the environment, and species which can be easily established with simple and inexpensive techniques (Benavides, 1991).

Data from producer surveys and the literature indicate the presence of woody forages in the humid tropics of the Atlantic Coasts of Costa Rica and Guatemala's Peten; in semi-arid areas nearby the south coast of Honduras and in the Dominican Republic; in the mountainous regions, with long drought periods and serious erosion problems in the Pacific slopes of Costa Rica; and in zones with temperate climates above 1000m in the high plateau of Guatemala and Costa Rica (Hernández y Benavides, 1993; Araya et al., 1993; Mendizábal et al., 1993; Godier et al., 1991).

Direct observation of animals eating, has allowed to identify species particularly palatable and with high digestibility (in vitro) of the organic matter and (IVOMD) and high crude protein (CP). These studies have permitted to value species without current use and to expand the utilisation of others normally used for other purposes (Hernández y Benavides, 1993; Godier et al., 1991; Reyes y Medina, 1992).

The information provided by producers has also allowed knowing simple agronomic management practices, easy to implement. An example of this are the woody forages identified in the Western Plateau of Guatemala, where, in most cases, the propagation is done by vegetative means (stem cuttings) with which a faster biomass can be obtained compared to sexual seed (Ruiz, 1992).

The forage from most of these woody forages shows PC values two and three times higher than tropical grasses and in some cases, even higher than commercial concentrates used to supplement ruminants. At the same time, the IVOMD is very high and comparable or superior to that of concentrates. Two species of the Euforbiaceae family are outstanding in nutritional quality, "Wide Chicasquil" (Cnidoscolus acotinifolius) and "Fine Chicasquil (C. chayamansa) , which leaves, with more than 30% CP and 75% IVOMD, are edible. Also stand out the nutritive value of two species of the Moraceae family, Mulberry (Morus spp.) and Amate (Ficus) from Petén, Guatemala; two from the Malvaceae family, the "Amapola" (Malvaviscus arboreus) and the "Clavelón" (Hibiscusrosa-sinensis); Black Sauco (Sambucusmexicana) and Yellow Sauco (S. canadensis), belonging to the Caprifoliaceae family; and three species of the Compositeae family, "Chilca" (Seneciosp.), "White Tora" (Verbesina turbacensis) and "Purple Tora" (V. myriocephala). All of these with CP higher than 20% and IVOMD higher than 70% (Araya et al., 1993; Mendizábal et al., 1993).
 

Animal Performance

In intake trials, "Poró" (Erythrina poeppigiana), was the most studied species in the 1980s, with observed values higher than 4% with lactating goats (Benavides, 1993). In other work, acceptability has been sought in forages species growing in semi-arid lands, under forests and in secondary forests, which were identified by means of direct observation of grazing animals (Godier et al., 1991; Hernández y Benavides, 1993; Reyes y Medina, 1993).

Both "Poró" and "Black Wood" (Gliricidia sepium), are legumes characterised by high CP content, but with lower IVOMD (Benavides, 1991). In those cases, research has shown that energy supplementation significantly improves animal performance (Benavides y Pezo, 1986) and that starch sources give a better response than simple sugars (Samur, 1984) .

With the species with higher nutritive value, the highest milk yields have been obtained, and a significant response has been observed when the foliage level is increased on grass based diets. That is the case with "Amapola" and Mulberry, with milk yields in goats of 2.2 and 4.0 kg/d, normally only possible with giving commercial concentrates. With these species, intakes higher than 5% of body are reported. With mulberry foliage, increasing weight gains have been observed when raising its proportion in the diet (Rojas y Benavides, 1994; López et al., 1993).

Of the know technologies, vegetative propagation if the most used, since shortens the establishment period, is easy to do and widely known by producers. Germination percentage exceed 95%, when stem cuttings of Mulberry and "Amapola" are used in humid tropic conditions (Araya y Benavides, 1992; Benavides et al., 1993; López et al., 1993). With the Yellow Sauco, nursery planting of stakes and further transplanting seems to be the most adequate propagation method (Araya y Benavides, 1992). In some species is possible to place stakes horizontally, obtaining several plants and sparing material (Esquivel, 1993). However, there are important variations among species, which are important to know before making a decision of which technique to use. (Strehle et al., 1992).

The association of leguminous trees and grasses is a viable alternative with two modalities. In the first, the forage of both grass and tree is utilised. Results of an experiment under humid tropical conditions, using King-grass (Pennisetum purpureum x P. typhoides) with Poró, where there was not nutrient replenishment, and all biomass was removed, showed that grass yields are not reduced with the presence of the tree, since tree pruning reduce competition for light. It was also found that digestible nutrient yields per area were triple compared with the grass alone. Nevertheless, in a short term for the grass and in the medium term for the "Poró", productivity declines due to lack of nutrient replenishment (Benavides et al., 1989).

The other way is to utilise associated Poro's foliage as green manure for the grass. Also under conditions of the humid tropics, grass yields improved when increasing amounts of Poro's foliage were applied. At the same time, the sole presence of the tree, even without pruning, stimulates grass growth compared to grass in monoculture without trees (Libreros et al., 1993a).

Traditionally in livestock husbandry, there is an unidirectional relationship between animals and plants, the first benefiting by obtaining the feed, but without contribution to its generation. En confined systems, it is possible to establish a two-way relationship, since most manure can be used as fertiliser. In this way a more balance system can be established where the plants benefit from animal excreta.

This particularly applies to those woody species with the best forage features. With their high biomass yields and without being able to fix nitrogen, they require high levels of fertiliser applications. In order to find a rational ecological solution, goats manure has been applied to "Amapola" and Mulberry plantations. The yields have been 18 and 30 t of dry matter per ha, respectively. With mulberry, yields go up with the years (Benavides et al., 1993).

An aspect of great importance, in sites with bimodal rainfall pattern, is tree-pruning techniques which allow abundant biomass production during the dry season. For this, the effects of pruning at the end of the dry period have been studied. In the Dominican Republic, pruning of Black Wood ("Piñon cubano" or Gliricidia) in the months of October, November and December, in addition to stop flowering, results in increasingly higher biomass yield during the dry period (Hernández, 1988). Similar results were obtained u other authors working with Amapola and Jocote (Spondiaspurpurea) (Rojas et al., 1992).

Up to now, most of the technologies have been implemented in small farms and in goat production systems oriented to family consumption. In these situations, in addition to the technology aspects, it is essential to know the economic viability of the alternatives developed, both at the station and on farm. For the economic aspects, partial budget analysis of experiments; profitability analysis (flow and net income) of implemented technologies in pilot modules; and analysis of family benefits and flow and net income at the farm level have been used. The analysis made so far, indicates that application of forage tree technologies on farm, is profitable and their presence contributes to improve family economy.

With dairy goats under a basal diet of grass, the use of Poró foliage and other agricultural by-products (e.g. reject bananas) like supplement, is more profitable than the use of concentrates, despite higher yields with the latter (Gutiérrez, 1985).

Total cost of dry matter, from planting to feeding, in some forage species, nutritionally comparable to commercial concentrates, is lower (Rojas, 1992). This in part explained the high profitability of a dairy goat model at CATIE with goats fed exclusively Mulberry and grass (Oviedo et al., 1994).

At the level of the family subsistence units, high profitability has been found when family labour is not considered, even with animal reproductive problems present (Martínez y Froemberg, 1992).
 

Environmental Impact of Introduced Technologies

Part of the research goals with forage trees is the development of planting techniques which allow soil conservation in erosion risk areas. At the same time, soil nutrient balance indicates if there is a need to add a nutrient with high extraction rate (Libreros et al., 1993).

The shrub species with high forage potential can also be used to control soil loss, since they can be planted at high densities, are perennial and can be associated with other crops. During three years, in a site with steep slope and with serious erosion problems, two types of Amapola plantations were established (Amapola in high density, in contour lines associated with low grass, and Amapola in more separated contour lines associated with maize) and were compared with a traditional maize crop (bare soil). Soil loss was much less with the Amapola plantations (Faustino, 1992).


II. Mulberry

Background

Mulberry is a shrub or a tree traditionally used for feeding the silkworm in various countries. It belongs to the Order Urticales, Moraceae Family and genus Morus. The better known species, Morus alba L. and Morusnigra L., seem to have their origin in the Himalayas' foothills (Soo-Ho et al., 1990). It has excellent nutritional value as forage. Benavides (1991) reported CP values higher than 20% and IVOMD above 80%.

The literature gives the following climatic ranges for Mulberry cultivation: temperature between 18 to 30° C; rainfall between 600 to 2500mm; photoperiod between 9 to 13 h/d; and relative humidity between 65 to 80% (Ting-Zing et al., 1988). Currently its cultivation is reported from sea level to 4000m, and it is reproduced by seed, stakes and grafting (Soo-Ho et al., 1990).

In Spain, Mulberry was recommended to be planted in association with other crops like maize, potatoes, vegetables, alfalfa and fruit trees, always controlling spacing and pruning to avoid light competition. Some authors recommend planting at 80 cm between plants and rows (González, 1951). In other articles, densities of 30,000 plants per ha for low pruning (below 70cm); 7-12,000 plants for medium pruning (70-170cm); and between 2,250 and 6,000 plants for high pruning (above 170cm) are mentioned (Ting-Zing et al., 1988).

The available yield information is almost exclusively on leaves, since it is the part used to feed the silkworm. In France, fresh leave yields of 17,000 kg/ha are reported with 7 x 7 m spacing. With higher densities, yields of 30,000 kg/ha have been obtained. Yields are related to plantation age and more specifically with trunk diameter (Secretain y Gaddo, 1934 cited by González, 1951). These authors report that annual leaf production in monoculture increases from 6,500 kg to 33,500 kg/ha from the first to the seventh year. In good land, leaf yield per plant varies from 9 to 70 kg when average trunk diameter increases from 7 to 55 cm (Secretain, 1924, cited by González, 1951). With 22.5 tons of human faeces and 300kg of ammonium sulfate, fresh leaf production can reach 13 ton/ha/year (Ting-Zing et al., 1988). In Paraguay, leaf yields of 20,000 kg/ha have been obtained in 4-year plantations harvesting at 30cm from the surface (Narimatsu y Kiyoshi, 1975).

Work with widely spaced plants in Turrialba (Costa Rica) a yield of 2.32 kg of dry matter per plant was calculated per year cutting at a height of 50 cm. Cutting at 1m, the yield decreased to 2.16kg. However, leaf production was 1kg for both cutting heights. With cuts every 60,120 and 180 days, total dry matter production was 1,64, 2,17 and 2,86 kg/plant/year, respectively. However, leaf production declined from 1.11 to 0.84kg between 60 and 180 days (Benavides, Esnaola y Borel, 1986).
 

Nutritional Value of Mulberry

Dry matter content. The nutritive value of Mulberry is one of the highest found in products of vegetable origin, far superior to traditional forages like alfalfa. Mulberry biomass is remarkable due to one characteristic which is found in very few plants; high levels of CP and high levels of digestible energy. It also notable for good mineral content and above all, its low fibre content.
 

Table 1. Dry matter, crude protein and IVDMD of Mulberry foliage and other feeds used in Central America (Espinosa , 1996)
 
Item
DM,%
CP,%
IVDMD,%
Mulberry (M. alba)
28.7
23.0
80.0
King grass (P. purpureum x P. typhoides)
20.0
8.2
52.7
Star grass (Cynodonlnemfluensis)
22.3
8.9
54.9
Commercial concentrate
91.5
17.7
85.0

The content of dry matter (DM) and other components in the leaves of Mulberry is higher when compared to traditional grasses used in animal feeding (Table 1). From Costa Rica there are reports of 25-32% DM in leaves; 23-29% for young stems; and 24-45% for woody stems (Benavides et al.,1996; Espinoza, 1996). In plantations of three Mulberry varieties planted at 0.40m between plants and 1m between rows (25,000 plants/ha), in three ecologically different sites in Costa Rica and with various fertilisation levels, it was found that DM content of leaves and edible stem were more affected by the location than by fertilisation level, without differences among varieties (Table 2).
 

Table 2. Site and nitrogen fertilisation effects on leaf dry matter content in three Mulberry (M. alba) varieties in Costa Rica (Espinosa, 1996).
 
Site
Variety, % DM
Mean2
Criolla
Indonesia
Tigriada
Puriscal
31.6
32.0
30.7
31.4b
Coronado
26.8
25.7
22.2
24.9c
Paquera
29.0
36.0
31.9
32.3a
N1, kg/ha/year
180
29.2
31.4
28.8
29.8a
360
28.9
31.5
28.6
29.7ab
540
29.2
30.8
27.4
29.1b
Mean2
29.1b
31.2a
28.3c
 

1 = Ammonium nitrate
2 = Values with same letter are not statistically different p < 0.05
 

Table 3. Site and nitrogen fertilisation effects on woody stem dry matter content in three Mulberry (M. alba) varieties in Costa Rica (Espinosa 1996)
 
Site
Variety, % DM
Mean1
Criolla
Indonesia
Tigriada
Puriscal
45.2
41.9
42.9
43.4b
Coronado
31.6
27.1
24.2
27.6c
Paquera
48.8
47.8
48.8
48.5a
N, kg/ha/year
180
42.7
39.2
39.8
40.6a
360
41.8
39.9
38.4
40.0a
540
41.2
37.8
37.8
38.9b
Mean1
41.9a
38.9b
38.6b
 

1 = Values with same letter are not statistically different, p<0,05.
 

In the woody stems, the differences were still more pronounced, with values between 27 and 48% of DM (Table 3). The small effect of fertilisation on DM was also reported in a plantation where various levels of goat manure were applied (Table 4).
 

Table 4. Dry matter content of Mulberry (Morus alba) by soil manure application (Benavides et al., 1994)
 
Fraction
% de MS
Manure level1
NH4NO31
0
240
360
480
480
Leaves2
26.4a
25.9bc
26.0b
25.5d
25.6cb
Young stem 
28.0a
27.1b
26.8b
26.0c
24.7d
Woody stem 
42.2a
41.5b
40.8c
40.4cd
40.0d

1 = kg de N/ha/year.
2 = Values with same letter horizontally do not differ statistically, p<0,001.
 

Mulberry leaves have high levels of CP and IVOMD when compared to other feed used in ruminant feeding. Data from Central America indicate CP values between 15-25% and IVOMD between 75-90%, which indicates a quality comparable or superior to commercial concentrates (Table 5). Non lignified stem also has a good nutritional quality, with CP values of 7-14% and IVOMD of 56-70% (Benavides et al., 1994; Espinoza, 1996; Rojas y Benavides, 1994)
 

Table 5. Site and nitrogen fertilisation effect on crude protein in three Mulberry (Morus alba) varieties in Costa Rica (Espinosa, 1996)
 
Site
Variety, % PC
Mean
Criolla
Indonesia
Tigriada
Puriscal
20.1
22.2
21.0
21.1b
Coronado
22.2
26.3
25.7
24.8a
Paquera
17.0
14.8
13.7
15.1c
N kg/ha/year
180
18.9
20.8
20.0
19.9a
360
20.5
20.8
19.9
20.4a
540
20.0
21.6
20.6
20.7a
Average
19.8a
21.1a
20.1a
 

As it was the case for DM, CP and IVDMD is similar among varieties and is not affected much by fertilisation nor by cutting frequency (Tables 6, 7, 8, 9, 10 and 11). Although, an effect was observed on CP content of leaves and young stem when ammonium nitrate was added instead of goat manure at iso-nitrogenous levels (Table 9).
 

Table 6. Site and nitrogen fertilisation effect on crude protein of young stem in three Mulberry (Morus alba) varieties in Costa Rica (Espinosa, 1996)
 
Site
Variety, % CP
Mean
Criolla
Indonesia
Tigriada
Puriscal
11.2
13.0
12.1
12.1a
Coronado
13.4
13.9
14.3
13.9a
Paquera
9.4
10.2
8.7
9.4b
N, kg/ha/year
180
11.2
12.7
11.6
11.4a
360
11.8
11.8
11.8
11.7a
540
11.3b
12.6a
11.7b
11.9a
Mean 
11.3b
12.4a
11.7b
 

Table 7. Site and nitrogen fertilisation effect of leaf IVDMD in three Mulberry (Morus alba) varieties in Costa Rica (Espinosa, 1996).
 
Site
Variety, % IVDMD
Mean
Criolla
Indonesia
Tigriada
Puriscal
78.0
75.5
76.8
76.7ª
Coronado
75.4
73.8
75.6
74.9ª
Paquera
71.6
71.2
71.6
71.5b
N, kg/ha/year
180
74.9
72.6
75.0
74.2ª
360
74.8
73.6
74.1
74.2ª
540
75.3
74.2
74.9
74.8ª
Year 
75.0a
73.5a
74.7a
 

Table 8. Site and nitrogen fertilisation effect on IVDMD of young stem in three Mulberry (Morus alba) varieties in Costa Rica (Espinosa, 1996).
 
Site
Variety, % IVDMD
Mean
Criolla
Indonesia
Tigriada
Puriscal
69.5
70.5
63.9
68.0a
Coronado
70.3
68.6
69.9
69.6ª
Paquera
65.9
64.7
59.4
63.3b
N, kg/ha/year
180
68.5
68.2
65.4
67.4ª
360
68.9
69.0
64.2
67.4ª
540
68.2
66.6
63.7
66.2ª
Average 
68.6a
67.9a
64.4b
 

Table 9. Manure application effect on crude protein content of Mulberry leaf and young stem (Benavides et al., 1994)
 
Fraction
Manure level1
NH4NO31
0
240
360
480
480
Leaves2
19.1c
19.3b
19.3c
20.2b
22.5ª
Young stem
7.4b
7.1b
7.2b
7.4b
12.3ª

1 = Equivalence in kg of N/ha/year.
2 = Values with same letter horizontally do not differ statistically, p<0,01.
 

Table 10. Manure application effect on dry matter digestibility of Mulberry leaf and young stem (Benavides et al., 1994)
 
Fraction
Manure level1
NH4NO31
0
240
360
480
480
Leaves
76.7
77.5
77.0
76.9
77.1
Young stem2
56.2b
56.4b
56.0b
55.8b
58.6ª

1 = Equivalence in kg of N/ha/year.
2 = Values with same letter horizontally do not differ statistically, p<0,01.
 

Table 11. Annual cutting frequency effect on digestibility and crude protein content of Mulberry biomass (Benavides et al., 1994)
 
Fraction
% CP
% IVDMD
3
4
3
4
Leaves1
19.2b
20.9a
77.2
76.9
Young stem
8.1
8.5
56.9
56.2

1 = Values with same letter horizontally do not differ statistically, p<0,01.
 

In the studies mentioned above, a marked site effect is reported, which is the result of the different soil and climatic conditions of each one. In Paquera, located in the Pacific Coast of Costa Rica, with high luminosity and high temperatures, leaf CP and IVDMD (15.1 and 71.5%, respectively) are reduced compared to higher locations with more clouds and lower temperatures ((24,8 y 74,9%, respectively), like is the case of Coronado and Puriscal, located in the mountainous areas of the country (Espinoza, 1996). The greater luminosity and higher temperature in Paquera can explain the lower water content in all fractions, lower CP and IVDMD levels, and high Dm production, as will be seen later. It is known that high luminosity reduces nitrate levels and increases cell wall components and growth due to larger photosynthetic activity (Van Soest, 1994). Coronado has lower temperatures and more cloudiness and rainfall, which could have limited growth and lignification. But the high soil fertility and the lower growth rates of this site, explain the higher CP and lower biomass DM. In addition to the climatic factors of Paquera, the lower N, copper and zinc soil contents could limit fertility and nutrient content in the plant. On the other side, the clay soils, the lower pH and the low potassium contents can explain low biomass yields in Puriscal.

Working with goats fed exclusively on Mulberry and "Amapola" leaves confined to metabolic cages, apart from high DM intake values, high levels of in vivo DM and CP digestibilities of Mulberry leaves (Table 12). The values reached 90% for leaf CP digestibility (Jegou et al., 1994).
 

Table 12. Dry matter and crude protein in vivo digestibilities of Mulberry and "Amapola" (Malvaviscus arboreus) with goats in metabolic cages (Adapted from Jegou et al., 1994)
 
Forage species
Parameters
Intake, % LW
IVDMD, %
IVCPD, %
Mulberry
4.18
79.3
89.5
Amapola
4.35
64.2
54.2

Although statistical comparisons have not been made, nitrogen, potassium and calcium contents of leaves and young stems are high (Tables 13 and 14), reaching values of 3.35, 2.0 and 2.5%, respectively (Espinoza, 1996). In other work (Table 15), no appreciable differences were found in mineral content of leaves and young stems when increasing amounts of legume leaves were added to the soil (Oviedo, 1995).

In a report of the proximate analysis of Kanvas-2 variety of Mulberry (Table 16), levels of protein (29.6%), ash (7.53%) and nitrogen-free extract (50.0%) were considered high. At the same time, low levels of crude fibre were reported (10.1%). In this work, the type and quantity of leaf amino acids were determined. Twenty four amino acids in significant concentrations were detected, plus 6 more at low concentrations (Table 17). The low fibre level and high contents of CP and IVDMD justify future evaluations of Mulberry as an ingredient for high quality meal and compounded feeds.
 

Table 13. Fertilisation effect on the mineral content of Mulberry (Morus alba) leaf in Costa Rica (Espinosa, 1996)
 
Variety
Minerals, % of Dry Matter
N
P
K
Ca
Mg
Criolla
3.17
0.30
2.07
1.90
0.47
Indonesia
3.37
0.30
1.73
2.87
0.63
Tigriada
3.23
0.40
2.33
2.74
0.55
N, kg/ha/year
180
3.18
0.33
1.96
2.54
0.55
360
3.26
0.33
2.05
2.49
0.55
540
3.32
0.33
2.12
2.48
0.55
Mean 
3.26
0.33
2.04
2.50
0.55

Table 14. Fertilisation effect on mineral content of Mulberry (Morus alba) young stem in Costa Rica (Espinosa, 1996)
 
Variety
Minerals, % of Dry Matter
N
P
K
Ca
Mg
Criolla
1.81
0.30
2.24
1.33
0.39
Indonesia
1.98
0.40
2.99
1.53
0.49
Tigriada
1.87
0.33
2.41
1.38
0.40
N, kg/ha/year
180
1.89
0.33
2.48
1.43
0.44
360
1.86
0.33
2.60
1.40
0.43
540
1.90
0.37
2.55
1.42
0.41
Mean
1.89
0.34
2.55
1.41
0.43

Table 15. Mulberry leaf mineral content as affected by level of Poro foliage added to the soil (Oviedo, 1995)
 
Mineral
Control Without trees
Proportion of Poro foliage
in the soil
Mean
0%
50%
100%
N
2.90
3.16
3.10
3.09
3.06
P
0.22
0.22
0.22
0.23
0.22
K
1.51
1.37
1.35
1.71
1.49
Ca
1.64
1.82
1.74
1.77
1.74
Mg
0.42
0.44
0.41
0.38
0.41

Table 16. Leaf composition of Mulberry variety Kanvas-1, in El Salvador, Central America (Coto, 1996).
 
Leaf position
Fractions, % of Dry Matter
Dry
matter
Crude
protein
Crude
fat
Crude
fibre
Ash
NFE
Terminal
19.5
33.6
3.2
9.4
7.7
46.1
Intermediate
22.5
28.3
2.8
10.2
7.3
51.4
Basal
23.0
26.7
3.4
10.8
7.7
52.6
Average
21.7
29.6
3.1
10.1
7.5
50.0

Table 17. Amino acid concentration in the leaves of Mulberry variety Kanva-1 in El Salvador, Central America (Coto, 1996)
 
Amino
acid
g/100g
of DM
Amino
acid
g/100g
of DM
Amino
acid
mg/100g
of DM
Alanine
1.2
Methionine
0.4
Glutathione
350 mg
Arginine
1.1
Phenyl–alanine
1.1
5Hydroxypipecolic ac.
36 mg
Aspartic acid
2.1
Proline
1.1
Pipecolic acid
10 mg
Cisteine
0.6
Serine
1.2
Sarcosine
0.5 mg
Glutamic acid
2.7
Threonine
0.9
Adenylic acid
Trace
Glycine
1.5
Tryptophan
0.3
Cytidylic acid
Trace
Histidine
0.6
Tyrosine
0.6
Guanylic acid
Trace
Isoleucine
1.4
Valine
1.4
Hydroxanthine
Trace
Leucine
1.8
Amino–benzoic ac.
8 mg
Trigoneline
Trace
Lysine
1.8
Choline
112 mg
Uridylic acid
Trace

 

Animal Response

Evaluations carried out with ruminants (cattle, goats and sheep) show high intakes levels of DM and high animal responses in weight gain and milk yield. Oviedo (1995) when comparing Mulberry foliage with concentrate (Table 18) as supplements to Jersey x Criollo grazing cows, found similar milk yields with both supplement (13.2 and 13.6 kg/animal/day, respectively) at equal DM intake levels (1.0% of LW) and superior to grazing only (11.3 kg/animal/day). Mulberry inclusion did not affect fat, protein and total solids contents in milk (Table 19), but improved net benefits when compared to the concentrate (US$ 3.29 vs. 2.84, respectively).
 

Table 18. Milk yield and dry matter intake of Jersey x Criollo cows grazing Star grass (Cynodon nlemfuensis) and supplemented with Mulberry or concentrate (Oviedo, 1995)
 
Feed consumed, % LW
Supplement
Concentrate
Mulberry
Nothing
Milk, kg/animal/day
12.45
12.08
10.34
Concentrate
1.0
   
Mulberry  
1.0
 
Star grass
3.7
3.4
3.7
Total
4.7
4.4
3.7

Table 19. Milk chemical composition from grazing cows supplemented with Mulberry or concentrates (Oviedo, 1995)
 
Component
Supplement
Concentrate
Mulberry
Nothing
Fat
3.95
3.81
3.63
Protein
3.53
3.51
3.31
Total solids
12.53
12.39
12.31

Esquivel et al. (1996), when replacing 0, 40 and 75% of the concentrate by Mulberry foliage, did not find significant differences (p<0.05) in milk production (14.2; 13.2 and 13.8 kg/animal/day, respectively) in Holstein cows grazing Kikuyo grass (Pennisetum clandestinum) without appreciable effects on milk quality (Table 20). Also in this work, considering only feeding costs, net income per animal was 11.5% higher with the maximum level of Mulberry than that obtained with concentrate only.
 

Table 20. Effect of the substitution of concentrate by Mulberry foliage on milk yield of Holstein cows grazing Kikuyo grass (Pennisetum clandestinum) (Esquivel et al., 1996)
 
Parameter
Relation Concentrate:Mulberry
100/0
60/40
25/75
Milk, kg/animal/day
14.2
13.2
13.8
Intake, kg DM/animal/day
Concentrate
6.4
4.2
1.9
Mulberry
0
2.8
5.5
Grass
9.3
7.8
6.2
Total
15.7
14.8
13.6

With cattle, attractive liveweight gains have been obtained when using Mulberry foliage as supplement. In the humid tropics of Turrialba (Costa Rica), Jersey x Criollo heifers grazing Star grass (Cynodon nlemfuensis) and supplemented with concentrate, Mulberry and concentrate or only Mulberry, no statistically differences were detected (p<0,05) among supplements (Table 21). The combination with Mulberry and concentrate gave the highest gains (742 g/animal/day) (Oviedo and Benavides, 1994).
 

Table 21. Intakes and liveweight gains of grazing dairy heifers supplemented with concentrate and Mulberry foliage (Oviedo, 1995)
 
Parameter
Only
concentrate
Concentrate
+ Mulberry
Mulberry
Liveweight gain
0.620
0.742
0.600
Intake of Mulberry, %LW
-
0.5
1.0
Intake of concentrate, % LW
1.0
0.5
-

With young Romo-sinuano bulls in total confinement and fed a basal diet of Elephant grass (Pennisetum purpureum), gains of 40, 690, 940 y 950 g/animal/day were observed with whole Mulberry DM intakes of 0, 0.90, 1.71 and 2.11% of LW as supplement (González et al, 1996). In this study, the benefit/cost relations were 0.10, 1.11, 1.18 and 0.97 for each of gain levels, respectively. The study lasted 70 days and animals were between 13-16 months old, with initial liveweight between 118 and 250 kg (Table 22).
 

Table 22. Effect of Mulberry supplementation on intake and liveweight gains of Romo-sinuano cattle in confinement and fed a basal diet of Elephant grass (González et al., 1996).
 
DM intake, % LW1
Mulberry dry matter offered, % LW
0
1.0
1.9
2.8
Fresh Mulberry
0.00d
0.90c
1.71b
2.11a
Elephant grass
2.04a
1.79a
1.29b
0.95b
Total diet
2.04c
2.69b
3.00a
3.06a
Weight gain, g/animal/day
0,04c
0,69b
0,94a
0,95a

1. Values with same letter horizontally do not differ statistically (p<0.05) with Tukey test.
 

With crossbred dairy goats of 40kg liveweight, Rojas and Benavides (1994) found milk yield increases of 2.0 and 2.5 kg/animal/day when Mulberry supplementation was raised from 1.0 to 2.6% of LW on DM basis (Table 23). There were slight increases of fat, protein and total solids contents (Table 24). In this study, high DM intakes and additive effect of Mulberry supplementation were observed. King grass was clearly substitute by Mulberry (Table 25).
 

Table 23. Effect of Mulberry supplementation on milk yield (kg/animal/day) of dairy goats (Rojas and Benavides, 1994).
 
Production
level
Level of Mulberry DM, % LW
Mean1
1.0
1.8
2.6
3.4
High
2.04
2.38
2.51
2.47
2.35
Low
1.64
1.82
1.91
2.12
1.87
Mean2
1.84b
2.10b
2.21ab
2.29ª
 

1 = Group averages differ statistically, p<0,0001.
2 = Values with same letter horizontally do not differ statistically, p<0,0001
 

Table 24. Effect of Mulberry supplementation on fat, protein and total solid contents of goats fed King grass (Adapted from Rojas and Benavides, 1994).
 
Fraction
Intake of Mulberry DM, % LW
 
1.0
1.8
2.6
3.4
Mean1
Fat
3.1
3.2
3.2
3.3
3.2
Protein
3.3
3.4
3.4
3.5
3.4
Total solids
10.7b
11.0b
11.4a
11.2ab
11.1

1 = Group means differ statistically, p<0,007.
 

Table 25. Effect of Mulberry supplementation on dry matter intake of confined dairy goats (Rojas and Benavides, 1994)
 
Forage
Dry matter intake, % LW
Mulberry
1.0
1.8
2.6
3.4
King grass
3,2a
2.9ab
2.6b
2.1c
Total
4.2c
4.7b
5.2ab
5.5a

1 = Group means differ statistically, p<0,0003.
2 = Values with the same letter horizontally do not differ statistically, p<0,0006
 

With Black Belly lambs receiving a basal diet of King grass, liveweight gains of 60, 75, 85 and 101 g/animal/ day were reported when Mulberry was given as supplement at 0, 0.5, 1.0 and 1.5% of LW on DM basis, respectively (Benavides, 1986). In this study, rather than a substitution effect, there was an additive effect of Mulberry on total DM intake (Table 26).
 

Table 26. Performance of Black Belly lambs fed various Mulberry levels (Benavides 1996)
 
Parameters
Mulberry level,
DM as %of LW
Significance
0
0.5
1.0
1.5
Initial weight, kg
15.7
15.8
15.8
15.1
 
Final weight, kg
21.5
22.6
24.4
25.6
 
Gain, g/day
60c
75b
85b
101ª
**
DM intake (kg/day)
King grass
0.66
0.60
0.62
0.60
 
Mulberry leaf
0
0.09
0.19
0.28
 
Total DM intake
0.66
0.69
0.81
0.88
**
DM intake, % LW
3.54
3.72
3.99
4.34
**
DM intake, g/kg/PV0.75
73.5
75.1
84.5
92.2
**

In a three-year evaluation (Table 27), in an agroforestry model with goats fed exclusively (at 3% LW in DM basis) with King grass and Mulberry, a lactation yield of 900 kg per 300 days was reported (Oviedo et al., 1994). This is equivalent to a mean of 3 kg/day and to 4.1 kg/day at the beginning of lactation. Forage came from a Mulberry and grass plantation associated with Poro (Erythrina poeppigina) measuring 1,100 m2, fertilised with goat manure, Poro foliage and feed rejects.
 

Table 27. Milk yield (kg/animal/day) in dairy goats fed exclusively grass and Mulberry foliage in the agroforestry model (Oviedo et al., 1994)
 
Month
1
2
3
4
5
6
7
8
9
Goat 1
3.22
3.46
3.47
3.41
2.65
2.69
2.23
2.44
2.53
Goat 2
3.41
3.93
3.53
3.44
2.91
2.67
2.68
1.86
1.71

During the third year, the module reached 5.0 kg/day, equivalent to 16,500 kg/ha/year (Table 28). The economic analysis indicated a benefit/cost relation of 1.27, 1.39 and 1.45 for each year, respectively (Table 29).
 

Ensiled Mulberry

One of the most serious problems of livestock husbandry in the tropics is the rapid decline of grass quality during the dry season. Among the most used alternatives is the conservation of forage by ensiling during the rainy season in order to use it in the dry season. However, silage is traditionally made with tropical grasses rich in fibre and low in soluble carbohydrates, which affects fermentation and results in a low quality product. Due to its low fibre and high level of carbohydrates, Mulberry foliage can be ensiled without additives, showing a lactic fermentation pattern and low CP losses (between 16-21% of CP in the final product) while maintaining between 66 ad 71% IVDMD (Vallejo, 1995; González et al., 1996). These parameters are far superior to silages made with tropical forages. Vallejo (1994), using 40kg plastic bags and three 30-day storage periods, compared the silage made from three tree foliages (Mulberry, Amapola and Jocote). Mulberry showed the highest levels of IVDMD, good CP content, acceptable loses of ammonium nitrogen and higher lactic acid levels (Table 30). Acetic and butyric acids levels were also high with Mulberry in the first period, but they rapidly declined (Table 31).
 

Table 28. Zootechnical parameters of confined goats in the agroforestry model in Turrialba, Costa Rica (Oviedo et al., 1994)
 
Parameter
Average
Year1
Year2
Year3
Lactation, months
14.61
     
Kids per parturition
2.0
     
Kid weight at birth, kg
3.7
     
Milk,, kg/animal/day 
2.02
     
Dry period, days
37.3
     
Goat weight, kg
50.0
     
Total milk, kg/year  
1223,03
1505,94
1110,55
Milk production, kg
Per module/day
4.0
3.4
4.2
5.0
Maximum monthly   
42.8
71.4
122.7
Minimum monthly   
145.1
189.1
207.9
Minimum per module/day  
1.4
2.3
4.0
Maximum per module/day  
5.2
6.1
6.7

1 = 3 lactations per goat.
2 = Data of 31.5 months.
3 = from 03/91 to 03/92.
4 = From 03/92 to 03/93.
5 = From 03/93 to 10/93.
 

Table 29. Financial analysis (in US$) of the goat agroforestry module at Turrialba, Costa Rica (Oviedo et al., 1994)
 
Description
Years
1991/92
1992/93
1993*
A. Costs      
A.1 Investments      
Mulberry x Poro plantation
4.61
4.61
2.88
Grass x Poro plantation 
1.66
1.66
1.04
Facilities
16.07
16.07
9.37
Animals
50.00
50.00
31.25
Subtotal
72.34
72.34
45.21
A.2 Fixed, land opportunity cost 
21.17
21.17
13.23
A.3 Variables (labour)      
Cut&carry, weeding, pruning
182.65
176.19
109.77
Forage chopping and feeding
138.45
133.55
83.20
Milking
89.05
85.90
53.52
Cleaning facilities
54.60
52.67
32.81
Manure application 
26.00
25.08
15.63
Mineral salts
30.66
30.66
19.16
Antelmintic
1.40
1.40
1.40
Maintenance
6.50
6.27
3.90
Subtotal
455.31
511.72
319.39
Total cost 
527.65
584.06
377.83
Updated cost 
610.82
643.92
396.72
B. Income      
B.1 Milk production 
672.66
813.99
549.03
Updated income 
778.68
897.42
576.48
C. B - A updated
167.86
253.50
179.76
B/C 
1.27
1.39