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Laboratory diagnosis


Serological tests


Competitive ELISA: most widely used. Based on a BT virus group-specific monoclonal antibody. Capable of detecting antibodies to all 24 serotypes of BT virus. Rapid, sensitive, easily standardised.

Agar gel immunodiffusion: simple but gives cross-reactions with related viruses such as EHD. Reading subjective.

Complement fixation: no longer widely used.