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Annex 3 - Educational objectives of the laboratory workshop

· Incubate and candle fertile eggs

· Inoculate fertile eggs by the allantoic cavity route

· Harvest allantoic fluid by an aseptic technique and test for sterility

· Obtain blood samples from chickens

· Process blood for serum or red blood cells

· Detect and titrate Newcastle disease virus haemagglutinin

· Detect and titrate haemagglutination inhibition antibodies against Newcastle disease virus haemagglutinin

· Vaccinate chickens by eye drop, mouth drop, nasal drop and injection

· Prepare food based vaccine

· Titrate the infectivity of preparations of Newcastle disease virus and calculate titres

· Prepare vaccines by a seed-lot system and prepare standard reagents for laboratory tests

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