I. | To be procured from UNDP | |
| | Quantity |
1. | Cannon AE 136 mm reflex camera with 1:1.5 lens | 1 |
2. | Cannon close-up lenses up to 1:1 magnification in macrophotography, a tube attachment for micro-photography, angle viewer and stand for close-up photography | 1 |
3. | Leitz Diavert inverted microscope, as per quotation and order (with specifications) | 1 |
4. | Reichert-Jung autocut microtome, standard outfit | 1 |
5. | Temtool automatic microtome knife sharpener | 1 |
6. | Reichert heating and cooling plate HK 120 | 1 |
7. | Deep freezer (-70° to -90°C) cabinet | 1 |
8. | Laminar flow sterile chamber | 1 |
9. | Table top refrigerator centrifuge (down to + 4°C) such as Beckman TF-6R | 1 |
10. | Leitz Orthoplan microscope with Fluotar Brightfield and Phase contant objectives NPL Fluotar, GW Eye-pieces 10 × binocular pluto tube, wide field phase contrast condenser system, Interference contrast device Y and tracing device | 1 |
11. | Field (portable) pH meter | 1 |
12. | Oxygen dispensers for fish transportation tanks | 5 |
II. | To be procured from CIFRI | |
1. | Incubator (temperature range 40°-60°C | 1 |
2. | BOD cabinet (range + 4° to + 40°C) | 2 |
3. | Refrigerators 280 1 (Kelvinator) | 2 |
4. | Hood (sterile chamber) | 2 |
5. | Magnetic stirrer (urgent) | 1 |
6. | Slide cabinet for 2 000 slides | 2 |
7. | Laboratory pH meter | 1 |
8. | Hot-air oven with thermostat and clock adjustment (ambient to 200°C) | 1 |
9. | Domestic pressure cooker 20 1 | 1 |
10. | Plant for triple glass distillation of water | 1 |
11. | Fish transportation tank, 1 m3 volume with sleeve for fish unloading | 1 |
12. | Fish transportation tank, 0.5 m3 volume with sleeve for unloading | 4 |
13. | Oxygen cylinders | 2 |
14. | Pressure reducers for oxygen cylinders, with manometers (regulators) | 2 |
15. | Sieves for fish sampling with leads, floats and ropes | 2 |
16. | Table cabinet for protection of fluorescent microscope from moisture and dust | 1 |
17. | Automatic slide projector | 1 |
18. | Cylinders with carbon dioxide for freeze microtone | 2 |
19. | Butane gas cylinders for providing gas flame in laboratories | 3 |
Bimonthly sampling should be followed throughout the year. Samples should be taken
from 2 to 3 most densely stocked ponds. Thirty specimens of each species are to be sent
in live or freshly dead condition. The laboratory should be notified at least one day
before the arrival of samples. Diagnosis and recommendations for treatments (if necessary)
will be sent officially not later than within seven days.
1. | WATER BODY |
1.1 | Name of pond, river or reservoir |
1.2 | Water quality (apparently normal, altered smell or colour, algae bloom, lack of oxygen suspected, others ….)1 |
1.3 | Water sample taken for analysis and sent |
2. | FISH (INCLUDING EGGS OR LARVAE) |
2.1 | Species and sizes affected by disease |
2.2 | Unaffected species and sizes in same water |
2.3 | Stress factors prior to disease outbreak (netting, stocking, sudden change in water temperature or oxygen concentration, others ….)1 |
3. | DISEASE |
3.1 | Date and hour when disease or mortality was first noted |
3.2 | Number of dead fish so far …. % …. |
3.3 | Behaviour of sick fish (restlessness, loss of balance, resting near surface or bank, loss of appetite, itching or flashing, others ….)1 |
4. | SAMPLES |
4.1 | Number of fish samples |
4.2 | Number of fish in each sample |
4.3 | Food samples |
4.4 | Water samples |
4.5 | Date and hour of taking sample |
4.6 | Date and hour of sending samples |
The laboratory should be notified as soon as possible about sending of samples.
Diagnosis and recommendations for treatment will be sent as soon as possible by the
quickest means and an official letter with these data will be sent not later than within
seven days.
1. | Broodfish |
1.1 | Number of broodfish available for spawning | |
1.2 | Number of broodfish that spawned | |
1.3 | Loss of broodfish immediately after spawning | |
1.4 | Loss of broodfish between two spawning seasons | |
1.5 | Suspected causes of losses (give approximate percentage) |
1.5.1 | Improper management | |
1.5.2 | Poaching | |
1.5.3 | Predators | |
1.5.4 | Diseases caused by parasites (name if possible) | |
1.5.5 | Other diseases (name if possible) | |
1.5.6 | Other (specify) | |
1.5.7 | Unknown | |
1.6 | Symptoms of diseases observed | |
1.6.1 | Emaciation | |
1.6.2 | Parasites | |
1.6.3 | Dropsy condition | |
1.6.4 | Blindness | |
1.6.5 | Any other (specify) | |
|
2. | Larvae to fry rearing |
2.1 | Total number of larvae stocked | |
2.2 | Stocking density (larvae/ha) | |
2.3 | Total number of fry fished out | |
2.4 | Suspected causes of losses | |
2.4.1 | Improper management | |
2.4.2 | Poaching | |
2.4.3 | Predators | |
2.4.4 | Parasites (Diseases caused by) | |
2.4.5 | Other diseases | |
2.4.6 | Other (Specify) | |
2.4.7 | Unknown | |
|
3. | Fry to fingerling rearing |
3.1 | Total number of fry stocked | |
3.2 | Stocking density (fry/ha) | |
3.3 | Total number of fingerlings fished out | |
3.4 | Suspected causes of losses | |
3.4.1 | Improper management | |
3.4.2 | Poaching | |
3.4.3 | Predators | |
3.4.4 | Parasites (Diseases caused by) | |
3.4.5 | Other diseases | |
3.4.6 | Other (Specify) | |
3.4.7 | Unknown | |
|
4. | Rearing from fingerling to market size |
4.1 | Total number of fingerlings stocked | |
4.2 | Stocking density (fingerlings/ha) | |
4.3 | Total number of fish harvested | |
4.4 | Suspected causes of losses | |
4.4.1 | Improper management | |
4.4.2 | Poaching | |
4.4.3 | Predators | |
4.4.4 | Parasites (Diseases caused by) | |
4.4.5 | Other diseases | |
4.4.6 | Other (Specify) | |
4.4.7 | Unknown | |