MANUAL FOR SPAWNING OF SEABASS, LATES CALCARIFER, IN CAPTIVITY

TABLE OF CONTENTS

FI:DP/MAL/79/018
Field Document 1
October 1984

A report prepared for the
Institutional Support for MAJUIKAN Aquaculture
Development Project

by

Tanan Tattanon and Banchong Tiensongrusmee


This report was prepared during the course of the project identified on the title page. The conclusions and recommendations given in the report are those considered appropriate at the time of its preparation. They may be modified in the light of further knowledge gained at subsequent stages of the project.

The designations employed and the presentation of the material in this document do not imply the expression of any opinion whatsoever on the part of the United Nations or the Food and Agriculture Organization of the United Nations concerning the legal or constitutional status of any country, territory or sea area, or concerning the delimitation of frontiers.

FOREWORD

The Government of Malaysia, assisted by the United Nations Development Programme and the Food and Agriculture Organization of the United Nations have been engaged in the project Institutional Support for MAJUIKAN1 Aquaculture Development (MAL/79/018).

The development objectives of the project are to accelerate the commercial development of aquaculture in Malaysia and to contribute to the achievement of the Government's objective of increasing the share of aquaculture in total fish landings from the present 3% to 50%, over the next 20 years, by enabling LKIM to demonstrate the viability of aquaculture projects and to provide overall guidance and direction in the development of the industry.

The immediate aims are:

The compilation of this manual is included in the project activities and was prepared by project staff Tanan Tattanon and Banchong Tiensongrusmee.

1 Fisheries Development Authority of Malaysia, renamed LKIM

ACKNOWLEDGEMENTS

The Food and Agriculture Organization is greatly indebted to all those who assisted the authors in the preparation of this manual, and particularly:

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS
Rome, 1984


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TABLE OF CONTENTS

1. INTRODUCTION

2. BROODSTOCK

3. SELECTION OF BREEDERS

4. SEX CHARACTERISTICS

5. INSPECTION OF STAGE OF MATURITY

6. INDUCED OVULATION

7. PROCESS OF HORMONE INJECTION

8. SPAWNING IN HATCHERY TANK

9. SPAWNING ACTIVITY

10. ARTIFICIAL FERTILIZATION

11. TRANSPORT OF FERTILIZED EGGS

12. CARE OF FERTILIZED EGG AND HATCHING

13. NURSERY TANK

14. FRY REARING

14.1 Salinity

14.2 Stocking density

14.3 Feed and feeding

14.4 Nursery tank management

14.5 Grading

15. GROWTH

16. CAUSES OF NURSERY MORTALITY

16.1 Cannibalism

16.2 Stocking density

16.3 Uneven growth

16.4 Disease infection

17. DISEASE CONTROL

17.1 Diagnosis

17.2 Preventive measures

17.3 Treatment

18. CULTURE OF LIVE FOOD

18.1 Tetraselmis

18.2 Chlorella

18.3 Rotifer

18.4 Marine yeast

18.5 Brine shrimp

18.5.1 Decapsulation

18.5.2 Hatching

18.6 Moina

19. PACKING AND TRANSPORT

20. SPERM PRESERVATION TECHNIQUE

REFERENCES

Appendix 1: METHOD OF COLLECTING SEABASS EGGS FROM SPAWNING TANKS

LIST OF TABLES

1. STAGES OF GONADAL DEVELOPMENT IN SEABASS, LATES CALCARIFER

2. SUITABLE RANGES OF WATER QUALITY OF THE BROODSTOCK TANK

3. EFFECTS OF SALINITY ON HATCHING RATE OF SEABASS EGGS

4. EMBRYONIC DEVELOPMENT OF SEABASS EGG AT 27°C

5. SURVIVAL RATE OF SEABASS LARVAE AT DIFFERENT SALINITIES

6. STOCKING DENSITY AND EXPECTED SURVIVAL RATE OF SEABASS FRY AT DIFFERENT AGE INTERVALS

7. TYPES AND RELATIVE AMOUNTS OF FOOD GIVEN AT DIFFERENT STAGES OF THE SEABASS LARVAE

8. PORE SIZE OF GRADER AND RETAINED SIZE OF FISH

9. NORMAL GROWTH OF SEABASS FRY IN 30 DAYS

10. DISEASE TREATMENT OF SEABASS FRY IN NURSERY TANK

11. NUTRIENTS FOR 1–1 STOCK CULTURE OF TETRASELMIS

12. NUTRIENTS FOR 3–1 CULTURE OF TETRASELMIS

13. NUTRIENTS FOR 200–1 AND 1-t CULTURES OF TETRASELMIS

14. NUTRIENTS FOR CHLORELLA CULTURE

15. NUTRIENTS FOR MARINE YEAST CULTURE

16. SURVIVAL RATE OF SEABASS FRY PACKED IN 40 × 60 cm PLASTIC BAGS

LIST OF FIGURES

1. Seabass spawning ground

2. Embryonic development of seabass

3. Seabass nursery tank

4. Seabass fry rearing tank

5. Water management and grading period for seabass rearing in hatchery

6. Rotifer culture showing algae peak, rotifer innoculation and rotifer peak

A1.1 Diagram of the two-stage egg collector