The purpose of this manual is to strengthen the use of good business practice in the field of environmental enterprise development. It provides guidance in planning enterprises so that they achieve financial viability, socioeconomic development for local populations, and conservation of the region’s natural resources—the triple bottom line. The manual is intended as a capacity-building resource to use enterprise as a strategy for the conservation of biological diversity and promote rural enterprise development with a social and environmental goal. The manual has four sections: Section 1: Planning environmental enterprise development; Section 2: Making an environmental enterprise work; Section 3: Forming environmental entrepreneurs; Section 4: Strengthening environmental enterprises.