1. Allen-Nelson “Miquel seawater”
Solution A | ||
KNO3 | 20.2 g/100 ml.H20 | |
Solution B | ||
Na2HP04.12H20 | 4.0 g | |
Cacl2.6H20 | 4.0 g | |
HCl conc. | 2.0 g | |
FeCl3 (melted) | 2.0 ml | |
Distilled water | 80.0 ml |
Add 2 ml. solution A to 1 liter seawater and 1 ml. of solution B to 1 liter of seawater.
II. Conwy Medium (Walne, 1974)
A. | Sodium Nitrate | 100 g |
EDTA, disodium salt | 45 g | |
Boric Acid | 33.6 g | |
Sodium Phosphate, monobasic | 20 g | |
Ferric Chloride, 6-hydrate | 1.3 g | |
Manganous Chloride, 4-hydrate | 0.36 g | |
Trace Metal Solution* | 1 ml | |
Vitamin Mix** | 100 ml | |
Distilled water (to make) | 1000 ml | |
(Note: use 1 ml Conwy medium/liter of seawater) * Trace Metal Stock Solution | ||
Zinc Chloride | 2.1 g | |
Cobalt Chloride, 6-hydrate | 2.1 g | |
Ammonium Molybdate, 4-hydrate | 2.1 g | |
Copper Sulfate, | 2.0 g | |
Distilled Water | 100 ml. | |
(Note: acidify with IN HCl until solution is clear) ** Vitamin Mix | ||
Vitamin B12 | 10.0 mg | |
Vitamin B1 | 20.0 mg | |
Distilled water | 200 ml |
III. TMRL Enrichment (Liao & Huang, 1970)
KNO3 | 100 g |
Na2HPO4.12H20 | 10 g |
FeCl3.6H20 | 3 g |
Na2SiO3.9H20 | 1 g |
Distilled water | 1000 ml |
(Note: Use 1 ml TMRL/liter of seawater) |
IV. Enrichment for Outdoor Cultures
16–20–0 | 12 g |
Urea 46 | 12 g |
21–0–0 | 100 g |
(Note: add above nutrients to 1 ton seawater) |
V. Modified F Medium (modified from Guillard & Ryther, 1962)
A. | N-P Stock (500x) | |
Sodium nitrate | 42.07 g | |
Sodium Phosphate, monobasic | 5.00 g | |
Distilled water (to make) | 1 liter | |
B. | Sodium Metasilicate Stock (500x) | |
Sodium metasilicate | 15.0 g | |
Distilled water (to make) | 1 liter | |
C. | Ferric Chloride Stock (500x) | 1.45 g |
Distilled water (to make) | 1 liter | |
D. | EDTA Stock (1000x) | |
EDTA, Disodium salt | 10.00 g | |
Distilled water (to make) | 1 liter | |
E. | Vitamin stock (1000x) | |
Thiamine HCl | 0.2 g | |
B12 | 10.0 ml* | |
Biotin | 10.0 ml** | |
* B12 stock (primary) | ||
-B12 | 0.1 g | |
Distilled | ||
water 1 liter | ||
** Biotin primary stock | ||
Biotin | 0.1 g | |
Distilled water | 1 liter | |
F. | Trace Metal Stock (1000x) | |
TM primary stock A | 1 ml | |
TM primary stock B | 1 ml | |
TM primary stock C | 1 ml | |
TM primary stock D | 1 ml | |
Distilled water (to make) | 1 liter | |
1) TM primary stock A solution | ||
Copper sulphate, 5-hydrate | 1.96 g | |
Zinc sulphate, 7-hydrate | 4.40 g | |
Distilled water (to make) | 100 ml | |
2) TM primary stock B solution | ||
Sodium molybdate, 2-hydrate | 1.26 g | |
or | ||
(Ammonium molybdate, 4-hydrate) | 0.907 g | |
Distilled water (to make) | 100 ml | |
3) TM primary stock C solution | ||
Manganous Chloride | 36.00 g | |
Distilled water (to make) | 100 ml | |
4) TM primary stock D solution | ||
Cobalt Chloride | 2.00 g | |
Distilled water (to make) | 100 ml |
NOTE: Dispense 2 ml each of solutions A, B, & C/liter of seawater
Dispense 1 ml each of solutions D, E, & F/liter of seawater.