MAGNESIUM GLUCONATE

Prepared at the 53rd JECFA (1999) and published in FNP 52 Add 7 (1999), superseding tentative specifications prepared at the 51st JECFA (1998), published in FNP 52 Add 6 (1998). Group ADI "Not specified" for glucono-delta-lactone and gluconates, established at the 51st JECFA in 1998.

SYNONYMS

INS No. 580

DEFINITION

The material of commerce exists as anhydrous, dihydrate or a mixture of both

Chemical names

Magnesium di-D-gluconate

C.A.S. number

Anhydrous: 3632-91-5
Dihydrate: 59625-89-7

Chemical formula

C₁₂H₂₂MgO₁₄

Structural formula

Formula weight

Anhydrous: 414.60
Dihydrate: 450.63

Assay

Not less than 98.0% and not more than 102.0% on the anhydrous basis

DESCRIPTION

White to off white, odourless, fine powder

FUNCTIONAL USES

Acidity regulator, firming agent, yeast nutrient, nutrient supplement

CHARACTERISTICS

IDENTIFICATION

Solubility (FNP 5)

Soluble in water; sparingly soluble in ethanol

Test for magnesium (FNP 5)

Passes test

Test for gluconate (FNP 5)

Passes test

PURITY

Water (FNP 5)

Between 3.0% and 12.0% (Karl Fischer Method)

Reducing substances (FNP 5)

Not more than 1.0% calculated as D-glucose (Method I)

Lead (FNP 5)

Not more than 2 mg/kg
Prepare a sample solution as directed for organic compounds in the Limit Test and determine by atomic absorption spectroscopy (FNP 5)

METHOD OF ASSAY

Dissolve about 0.6 g of the sample, accurately weighed, in 50 ml of water, add 10 ml of ammonia/ammonium chloride buffer solution and 5 drops of eriochrome black TS. Titrate with 0.05 M disodium ethylenediaminetetraacetate to a deep blue colour.

Calculate % magnesium gluconate, dihydrate (as is basis) from:

where

45.07 = equivalence factor for magnesium gluconate, dihydrate

	Prepared at the 53rd JECFA (1999) and published in FNP 52 Add 7 (1999), superseding tentative specifications prepared at the 51st JECFA (1998), published in FNP 52 Add 6 (1998). Group ADI "Not specified" for glucono-delta-lactone and gluconates, established at the 51st JECFA in 1998.
SYNONYMS	INS No. 580
DEFINITION	The material of commerce exists as anhydrous, dihydrate or a mixture of both
Chemical names	Magnesium di-D-gluconate
C.A.S. number	Anhydrous: 3632-91-5 Dihydrate: 59625-89-7
Chemical formula	C₁₂H₂₂MgO₁₄
Structural formula
Formula weight	Anhydrous: 414.60 Dihydrate: 450.63
Assay	Not less than 98.0% and not more than 102.0% on the anhydrous basis
DESCRIPTION	White to off white, odourless, fine powder
FUNCTIONAL USES	Acidity regulator, firming agent, yeast nutrient, nutrient supplement
CHARACTERISTICS
IDENTIFICATION
Solubility (FNP 5)	Soluble in water; sparingly soluble in ethanol
Test for magnesium (FNP 5)	Passes test
Test for gluconate (FNP 5)	Passes test
PURITY
Water (FNP 5)	Between 3.0% and 12.0% (Karl Fischer Method)
Reducing substances (FNP 5)	Not more than 1.0% calculated as D-glucose (Method I)
Lead (FNP 5)	Not more than 2 mg/kg Prepare a sample solution as directed for organic compounds in the Limit Test and determine by atomic absorption spectroscopy (FNP 5)
METHOD OF ASSAY	Dissolve about 0.6 g of the sample, accurately weighed, in 50 ml of water, add 10 ml of ammonia/ammonium chloride buffer solution and 5 drops of eriochrome black TS. Titrate with 0.05 M disodium ethylenediaminetetraacetate to a deep blue colour. Calculate % magnesium gluconate, dihydrate (as is basis) from: where 45.07 = equivalence factor for magnesium gluconate, dihydrate