Prepared at the 53rd JECFA (1999) and published in FNP 52 Add 7 (1999), superseding tentative specifications prepared at the 37th JECFA (1990), published in FNP 52 (1992). ADI "Not specified" established at the 37th JECFA in 1990. | |
SYNONYMS |
Rennin, milk-clotting enzyme, chymosin, chymosin B, aspartyl protease |
C.A.S. number |
9001-98-3 |
SOURCES |
Produced extracellularly by the controlled fermentation of Kluyveromyces lactis containing the bovine prochymosin B gene. The fermentation broth is adjusted to pH 2 to convert prochymosin to chymosin, which also kills the production cells. Chymosin is separated from the cell material by filtration and further purified by clarification with diatomaceous earth followed by several cellfiltrations and one ultrafiltration. |
ACTIVE PRINCIPLES |
Chymosin |
SYSTEMATIC NAMES AND NUMBERS |
None (EC 3.4.23.4) |
REACTIONS CATALYZED |
Cleaves a single bond in kappa-casein |
DESCRIPTION |
Clear, colourless or slightly coloured aqueous solutions containing the active enzyme |
FUNCTIONAL USES |
Enzyme preparation |
GENERAL SPECIFICATIONS |
Must conform to the General Specifications for Enzyme Preparations used in Food Processing (FNP 52 & Addendum 6) |
CHARACTERISTICS |
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IDENTIFICATION |
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Milk clotting activity (FNP 5) |
The sample shows milk clotting activity |