A Basic Laboratory Manual for the Small-Scale Production and Testing of I-2 Newcastle Disease Vaccine

RAP publication 2002/22

Sally E. Grimes

ISBN 974-7946-26-2

Table of Contents

The designations employed and the presentation of the material in this publication do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations (FAO) nor the Animal Production and Health Commission for Asia and the Pacific (APHCA) concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries.

All right reserves. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying or otherwise, without the prior permission of the copyright owner. Applications for such permission, with a statement of the purpose and extent of the reproduction, should be addressed to the FAO Regional Office for Asia and the Pacific (RAP), Maliwan Mansion, 39 Phra-Atit Road, Bangkok 10200, Thailand

© FAO-APHCA 2002

For copy of the manual and correspondence, please contact:

Senior Animal Production and Health Officer and Secretary of APHCA
FAO Regional Office for Asia and the Pacific (RAP)
39 Maliwan Mansion, Phra-Atit Road
Bangkok 10200, THAILAND

E-mail: [email protected]
FAO Headquarters' Homepage: http://www.fao.org
FAO-RAP Homepage: http://www.fao.or.th
APHCA Homepage: http://www.aphca.org

Table of Contents




1. Newcastle disease vaccines: an overview

2. Basic laboratory skills

3. Laboratory safety: an overview

4. Incubation of fertile eggs

5. Candling eggs

6. Inoculation of embryonated eggs by the allantoic cavity route

7. Collection of blood from chickens

8. Preparation of washed chicken red blood cells

9. Harvesting and storage of allantoic fluid

10. Haemagglutination test

11. Serology

12. Titrating Newcastle disease virus for infectivity

13. The production of I-2 Newcastle disease vaccine

14. Quality control: an overview

15. Isolation of virulent Newcastle disease virus

16. Cleaning and decontamination

17. Frequently asked questions

18. Glossary

19. Bibliography

Appendix 1. Newcastle disease: an overview

Appendix 2. Newcastle disease virus: classification and important viral proteins

Appendix 3. Media and solutions

Appendix 4. Two-fold serial dilutions

Appendix 5. Ten-fold serial dilutions

Appendix 6. Calculation of arithmetic and geometric means

Appendix 7. Dilutions

Appendix 8. Notes on centrifugation

Appendix 9. Notes on the anatomy of the embryonated egg

Appendix 10. Calculation and recording sheets