Annexes

ANNEXE-2

Descriptor for silkworm, Bombyx mori.

COLLECTION DATA

1. Collector's number (Original number assigned: by the collector to the sample)
2. COLLECTING INSTITUTE:
3. DATE OF COLLECTION OF ORIGINAL SAMPLE:
4. COUNTRY/PROVINCE/STATE OF COLLECTION:
5. LOCATION OF COLLECTION SITE

a) Wild: Distance & direction of: from the nearest town/village
b) Postal address of the donor:

6. LATITUDE OF COLLECTION SITE:
7. LONGITUDE OF COLLECTION SITE:
8. ALTITUDE OF COLLECTION SITE:
9. COLLECTION SOURCE (Wild/Farm/Institution):
10. LOCAL/VERNACULAR NAME:
11. NUMBER OF SPECIMENS SAMPLED:
12. PHOTOGRAPH:
13. MUSEUM SPECIMEN:
14. DISEASE STATUS:
15. END USE

ANNEXE-3

Descriptor for silkworm, bombyx mori.

ACCESSION DATA:

1. ACCESSION NUMBER:
2. DONOR NAME:
3. DONOR IDENTIFICATION NUMBER:
4. OTHER NUMBERS ASSOCIATED WITH ACCESSION:
5. SCIENTIFIC NAME(Genus sp. ssp. Race):
6. PEDIGREE:
7. ACQUISITION (Month and year):
8. DATE OF LAST REARING/MULTIPLICATION:
9. ACCESSION SIZE (How many DFLs are maintained in donor institution)
10. NUMBER OF GENERATIONS REARED:
11. TYPE OF MAINTENANCE (Cellular/Mass):
12. COUNTRY OF ORIGIN:
13. SITE OF MAINTENANCE:
14. CURATOR:
15. LOCAL NAME (At rearer's level):
16. DISTRIBUTION:
17. GRS RACE/MUTANT/VARIANT NAME:
18. PATENT NUMBER:
19. SYNONYMS:

ANNEXE-4

Descriptor for silkworm, Bombyx mori.

SPECIMEN DATA
From Donor

1. HABITAT (Including climatic conditions: Temperature, humidity, rainfall etc. and seasons: Summer, rainy and winter)

2. HABIT:

3. NAME OF THE STATION:

4. DATE OF COLLECTION:

5. PLACE OF COLLECTION:

6. YEAR OF AUTHORISATION/RELEASE:

7. BREEDER'S NAME:

8. GEOGRAPHICAL/EVOLVED RACE/OBSOLETE etc.:

9. PARENTAGE:

10. VOLTINISM:

11. FECUNDITY:

12. HATCHING %:

13. LARVAL PATTERN:

14. EATING BEHAVIOUR:

15. MOULTING BEHAVIOUR:

16. V-AGE LARVAL DURATION:

17. TOTAL LARVAL DURATION:

18. COCOON COLOUR:

19. COCOON SHAPE:

20. PUPATION RATE:

21. COCOON WEIGHT:

22. SHELL WEIGHT:

23. SHELL RATIO:

24. FILAMENT LENGTH:

25. DENIER:

26. NEATNESS:

27. REELABILITY:

28. BOIL-OFF LOSS:

ANNEXE-5

Descriptor - characterisation

EGG:

1. Egg shape
2. Chorion colour
3. Serosa colour
4. Yolk colour
5. Glue

LARVA:

6. Colour of newly hatched larva
7. Colour of V instar larva
8. Integument
9. Body shape
10. Body built
11. Markings
12. Haemolymph colour

COCOON:

13. Colour
14. Shape
15. Built
16. Grains
17. Layer

PUPA:

18. Colour
19. Shape
20. Development of wing pad
21. Development of thoracic leg

ADULT:

22. Moth colour
23. Body shape
24. Colour of antenna
25. Shape of antenna
26. Eye colour
27. Wing fasciation

ANNEXE-6

Descriptor - States for silkworm (Bombyx mori. L) - characterisation
Reference guide for recording morphological character data

S.No	Descriptor	METHOD OF OBSERVATION	DESCRIPTOR-STATES
1	Egg colour	Denotes the colour of chorion of freshly laid egg. Observed under diffused sunlight within 24 hours after oviposition under naked eye	White Yellow Mixed
2	Egg shape	Observed under stereo dissection microscope	Ellipsoidal
3	Yolk colour	Observed by piercing freshly-laid eggs and immediately observing under stereo dissection microscope under diffused sunlight.	Yellow Greenish yellow
4	Serosa colour	The colour (pigmentation) of embyonic membrane round outside the yolk during amniotic cavity development. Observed under diffused sunlight in hibernating eggs through naked eye	Brown Grey Olive green Mixed
5	Colour of neonate	Observed in newly hatched larva (neonate) after eclosion under no feeding.Usually observed through stereo dissection microscope under diffused sunlight.	Brown Dark brown Mixed Chocolate
6	Type of bristle	Observed with stereo dissection microscope under diffused sunlight. The larva is observed for the length and density of setae over the dorsal surface	Normal Dense Short
7	Eye spot mark in v instar	Observed with naked-eye during 3day of V instar on the II thoracic segment.The intensity of impression to be recorded.	Absent Faint Dark Mixed
8	Crescent spot mark in v instar	Observed with naked-eye during 3rd day of V instar on the II abdominal segment. The intensity of impression to be recorded.	Absent Faint Dark Mixed
9	Star spot mark in v instar	Observed with naked-eye during 3rd day of V instar on the II abdominal segment. The intensity of impression to be recorded.	Absent Faint Dark Mixed
10	Other markings on V instar larva	Observed with naked-eye during 3rd day of V instar on the last 4 - 5 abdominal segment. The intensity and the nature of impression to be recorded.	Quail mark absent Quail mark present Quail (Present&absent) Zebra mark
11	Body shape of v instar	Observed with naked-eye during 3rd day of V instar. The length, cuticular projections re to be noted	Cylindrical Knobbed
12	Body built of v instar	The stoutness and slenderness of larva on the 5th day of V instar to be recorded.	Medium stout Slender Stount Stout (female) Medium stout (Male)
13	Body colour of v instar	The overall colour of the dorrsal area.Observed with naked-eye during 3rd day of V instar under diffused sunlight.	White Dirty Ash or Smoke colour Mixed
14	Colour of haemolymph	Observed under diffused sunlight with naked-eye by collection haemolymph from prolegs in thiourea solution	Colourless Yellow Mixed
15	Nature of integument of v instar	It is the transluecy of the cuticle indicating the opaqueness or transparency or oilyness. Observed with naked-eye during 4th day of V instar	Moderately translucent Opaque Translucent Mixed Oily
16	Cocoon colour	The colour of the outer shell devoid of floss. Observed with naked-eye on 4th day of cocooning under diffused sunlight	Golden yellow Greenish yellow White Yellow Mixed Straw Flesh coloured
17	Cocoon shape	Observed in fully developed cocoons and matching with the standard shapes	Oval Elongated oval Oval with constriction Elongated Elongated with constriction Spatulate Spindle
18	Cocoon built	Recorded by pressing the cocoon along sides with thumb and index-finger with slight pressure	Hard Medium Soft
19	Cocoon texture	It is the amount of coarseness or smoothness of the shell surface and extent of floss deposition, shining over it. It is observed using the hand lens or with stereo microscope	Smooth Flossy Velvetty
20	Nature of cocoon grains	It is the size and the quantum of ridges over the shell, usually per square cm. Observed with hand lens or stereo microscope.	Fine Medium Coarse Flossy
21	Pupal colour	Observed under diffused sunlight with naked eye for each sex.	Brown Brown (Male), Light Brown (Female) Dark brown Black
22	Pupal shape	Observed after cutting open cocoon and matching with standard shapes	Elliptical Knobbed
23	Development of wing pad & legs in pupa	Pupa is observed for complete development of wing pad and legs on the 7th day of pupation(MV) and 9th day in bivoltine	Developed Vestigial Absent
24	Body colour of moth (male)	Observed in newly emerged moths under diffused sunlight	Dull white Dirty
25	Body colour of moth (female)	Observed in newly emerged moths under diffused sunlight	Dirty Dull white
26	Body shape of moth	Observed in newly emerged moths under diffused sunlight	Elliptical
27	Shape of antenna in moth	Observed in newly emerged moths under diffused sunlight	Bipectinate
28	Antenna colour in moth	Observed in newly emerged moths under diffused sunlight	Black Brown Yellowish brown
29	Eye colour of moth	Observed in newly emerged moths under diffused sunlight	Black Red

ANNEXE-7

Descriptor - evaluation

EGG:

1. Fecundity (No.)
2. Hatching(%)
3. Unfertilized egg(%)
4. Unhatched egg(%)
5. Incubation period (days)

LARVA:

6. Weight of 10 grown larvae (g)
7. V-instar duration (h)
8. Total larval duration (h)

COCOON/PUPA:

9. Cocoon weight (g)
10. Shell weight (g)
11. Shell ratio (%)
12. Cocoon yield by no./10,000 larvae
13. Cocoon yield by wt./10,000 larvae
14. Pupation rate (%)
15. Cocoon/litre (No.)
16. Cocoon length/width (cm)
17. Melt cocoon (%)
18. Double cocoon (%)
19. Open-end cocoon (%)
20. Stained cocoon (%)
21. Pupal weight (g)
22. Floss(%)

RAW SILK

23. Silk filament length (m)
24. Non-Broken filament length (m)
25. Denier (d)
26. Reelability (%)
27. Renditta
28. Raw silk recovery (%)
29. Silk waste (%)
30. Raw silk (%)
31. Neatness (%)
32. Boil of loss (%)
33. Cleanness (%)
34. Evenness variation-I
35. Tenacity (g/d)
36. Elongation (%)
37. Cohesion (no.of strokes)
38. Low neatness (%)

ANNEXE-8

Descriptor - States for silkworm (Bombyx mori. L) - evaluation

Descriptors and method of evaluation for important economic parameters

Sl No.	Descriptor	Method of evaluation
	I. Egg descriptors
1.	Fecundity (No.)	The mean number of eggs laid by a female moth, recorded as fecundity from five disease-free layings per accession.
2.	Hatching (%)	The mean number of larvae hatched out of the total eggs laid by a moth recorded in five layings per accession and represented in percentage.
3.	Unfertilised egg (%)	The mean number of unfertilised eggs to the total eggs laid by a moth recorded from five layings per accession and represented in percentage
4.	Unhatched egg (%)	The mean number of unhatched (dead) eggs to the total eggs laid by a moth recorded from five layings per accession and represented in percentage.
5.	Incubation period (days)	The mean period in days recorded from the day of oviposition to eclosion in non-hibernating races, while in hibernating races it is the period (days) from date of release of eggs from cold storage to eclosion.
	II. Larval Descriptors
6.	Weight of 10 grown larvae (g)	Mean larval weight (g) recorded for 10 randomly selected larvae at the peak growth of fifth age larvae from each bed/replication
7.	Fifth instar larval duration (h)	Mean larval span of fifth instar larva recorded in hours.
8.	Total larval duration (h)	The mean larval span from its first feeding to pre-spinning stage, recorded in hours.
	III.Cocoon descriptors
9.	Single cocoon weight (g)	The mean single cocoon weight (g) arrived from 10 cocoons each of male and female randomly selected per accession.
10.	Single cocoon shell weight (g)	The mean single shell weight (g) arrived from randomly selected 10 cocoons each for male and female per accession.
11.	Shell ratio [%]	The mean ratio of shell weight to that of cocoon weight calculated from mean cocoon and shell weight respectively and represented in percentage.
12.	Cocoon yield (No.)/10,000 larvae	The mean number of cocoons harvested to that of larvae retained and converted for 10,000 larvae.
13.	Cocoon yield (Wt.)/10,000 larvae	The mean weight of cocoons harvested in kg. to that of larvae retained and converted for 10,000 larvae.
14.	Pupation rate %	the mean number of live pupae obtained to that of larvae retained and represented in percentage.
15.	Cocoon per litre (No.)	The mean number of cocoons recorded by accommodating the cocoons in one litre volume container.
16.	Cocoon length (cm)	The mean length in cm calculated by recording the length of 50 individual cocoons selected randomly per accession.
17.	Cocoon width (cm)	The mean width in cm calculated by recording the width of 50 individual cocoons selected randomly per accession.
18.	Melt cocoons %	The mean number of dead/melt pupae containing cocoons obtained to that of larvae retained and represented in percentage.
19.	Double cocoons %	The mean number of double pupae containing cocoons obtained to that of larvae retained and represented in percentage.
20.	Open-end cocoons %	The mean number of open-end cocoons obtained to that of larvae retained and represented in percentage.
21.	Stained cocoons (%)	the mean number of stained cocoons obtained to that of larvae retained and represented in percentage.
22.	Pupal weight (gm)	The mean weight (g) of single pupa, arrived from recordal of weighing 10 pupae each of male and female selected randomly per accession.
23.	Floss %	The mean ratio of single cocoon floss weight to that of single shell weight represented in percentage.
	IV. Post cocoon descriptors
24.	Averagel filament length (m)	Average filament length indicates the reelable length of silk filament from the cocoon. It is the avg..length of the silk reeled from the cocoons. * No. of reeling cocoon = No. of cocoons taken for testing - No. of converted unreelable cocoons + No. of converted carry over cocoons.
25.	Non-broken filament length (m)	It is the length at which cocoon replacement or break occurs in that interval. # No. of feeding ends = No. of castings + No. of carry over cocoons - No. of converted carry over cocoons.
26.	Single cocoon filament denier (d)	The size of the cocoon filament expressed as Wt. In grams of 9000 mtrs. length. OR
27.	Degumming loss (%)	The % of loss of wt. Due to removal of silk gum or sericin formula:
28.	Raw silk %	Quantity of raw silk reeled in relation to the quantity of fresh cocoons utilized, expressed in %.
29	Reelability (%)	It is the ratio of non-breakable filament length × filament length expressed in %. * No.of cocoons taken for testing - no.of new unreelable cocoons - no.of converted carry over cocoons. # No.of castings + no.of carry over cocoons - no. of converted carry over cocoons.
30	Renditta	It is the quantity of cocoons (kgs) required to produce one kg. of raw silk.
31	Raw silk recovery %	It is the ratio of raw silk percent to the shell ratio % or it is the quantity of raw silk recovered from the cocoon shell.
32	Neatness %	This is to determine the neatness % of raw silk, the defects are smaller than those classified as minor defects under cleanness test, such as loops, hairyness, raw knots and fine corkscruews.
33	Cleanness %	The object of this test is to determine the kind and no. of cleanness defects of raw silk. Defects are divided into 3 groups, super, major defects, major defects and minor defects such as long knots, long loops, cork screws, bad casting, waste, slugs etc.
34	Evenness variations - I,II,III	There are 3 types of evenness variations - i, ii, iii. The object of this test is to determine the degree and frequency of size variation occuring in the raw silk thread. Evenness variation is defined as the defective portion of raw silk threads on an inspection board which appear as stripes caused by variations in the size of raw silk and is noticeable by visual inspection. The degree of such variation is called intensity of variation. The intensity of variation is compared with the standard board consisting of Std.variation photographs, viz., Vo, V1, V2.
35	Tenacity (g/d)	Tenacity is the strength expressed as grams per denier.
36	Elongation (%)	It is the ratio when the raw silk is stretched before it breaks.
37	Cohesion (no.of strokes)	The object of this test is to determine the degree of agglutination of cocoon filament forming the thread. The instrument used to test the cohesion is duplon cohesion tester. The no.of strokes required to open the individual filaments are recorded, lesser the strokes poorer the cohesive property and vice-versa. Cohesive property is important because it directly impairs the fabric quality if the yarn is not having sufficient cohesiveness.
38	Waste % on silk weight.	It is the ratio of the weight of the waste obtained during cooking and reeling to the weight of the raw silk reeled.

ANNEXE-10

SAMPLE PAGES OF CSGRC WEBISTE (www.silkgermplsm.com)

CENTRAL SERICULTURAL GERMPLASM RESOURCES CENTRE

About us Mandate/Activities Mulberry Germplasm Silkworm Germplasm Contact Us

~ ABOUT US ~

The Central Sericultural Germplasm Resources Centre (CSGRC) was established in 1990 by the Central Silk Board, Government of India. We maintain more than 900 mulberry and 350 silkworm germplasm resources.

Catalogues are available for 328 mulberry and 225 silkworm germplasm. More than 100 descriptors in mulberry and 70 descriptors in silkworm are currently under investigation.

This would make CSGRC one of the largest repositories of sericultural germplasm in the world.

We would welcome suggestions and co-operation from scientific institutions, environmentalists and researchers for strengthening our collection as well as utilization of available resources.

Location

CSGRC is located at Hosur in Tamil Nadu approximately 40 km from Bangalore. Nearest Airport and Railway station are at Bangalore.

Geographical location

Altitude:942meters above MSL
Latitude: 12° 45" N
Longitude: 77° 51" E
Average Maximum Temperature: 27-36°C
Hottest month: April
Average Minimum Temperature: 9-14°C

Coldest month: December
Average Annual Rainfall: 800-1000 mm