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ANNEX I

FIELD VISITS RELATED TO SHRIMP DISEASE INVESTIGATION

RemarksPlaceStaffDate
Emergency visit on AI's requestAin KaamGuibli/ Enbaya/Abu Issa27.07.94
Technical visitAin KaamVallet/ Enbaya/Guibli14.08.94
To obtain complementary information on the diseaseAin KaamVallet/ Guibli/Enbaya22.08.94
Samples of affected shrimps preserved in 50 % ethanolAin KaamVallet/ Enbaya/Guibli05.09.94
5 samples of shrimps, kept in cold waterAin KaamVallet/Tresh15.09.94
3 samples of shrimps (cold water) to provide specimen to microbiology lab. for analysisAin Kaam & Tripoli Veterinary CollegeVallet/Tlheyi/ Fituri/Zoghdani/ Hashad08.10.94
4 samples (live) of shrimp (cold water) and 2 lispafish shrimpsAin kaamVallet/Alturky27.10.94
To organise cooperation MBRC/Vet.Col. Follow-up examinations.Vet. Col.A. FituriVarious visits. (More than 25 times)
To inform the AI ManagementAin KaamVallet/ Alturky/Ragig07.11.94

ANNEX II

RECOMMENDED REFERENCES

B. Austin & D.A. Austin. 1989 Methods for the Microbiological Examination of Fish and Shellfish. Ellis Horwood Ltd / John Wiley & Sons.

B Austin and D.A. Austin. 1987 Bacterial Fish Pathogens; disease in farmed and wild fish. Ellis Horwood Ltd / John Wiley & Sons.

Carl J. Sindermann and Donald V. Lightner. 1989 Disease Diagnosis and Control in North American Marine Aquaculture. Elsevier, Amsterdam.

Carl J. Sindermann. 1970, 1990 Principal Diseases of Marine Fish and Shellfish. 2 Vol. Academic Press.

Chris Andrews, Adrian Exell and Neville Carrington. 1988 The Manual of Fish Health. Salamander Books.

David B. Groman. 1982 Histology of the Striped Bass. American Fisheries Society, Monograph No.3, ISSN 0362–1715

Frank O. Perkins & Thomas C. Cheng. 1990 Pathology in Marine Science. Academic Press, Inc.

George Post. 1983 Textbook of Fish Health. T.F.H.Publications, Inc. Ltd.

Hans-Joachim Lange. 1983 Methods of Analysis for the Canning Industry. Food Trade Press.

M.K. Refai. 1981 Manuales para el control de calidad de los alimentos, 4: anàlisis microbiologico. FAO, Rome.

Otto Kinne. 1980 Diseases of Marine Animals. Vol. I. John Wiley & Sons.

Thomas A. Bell, Donald V. Lightner and William C. Randall. 1988 A Handbook of Normal Penaeid Shrimp Histology. Aquaculture Dev. Prog.; Dept. Land & Nat. Res; State of Hawaii; World Aquaculture Society.

Valerie Inglis, Ronald J. Roberts, and Niall R. Bromage. 1993 Bacterial diseases of fish. Blackwell Scientific Publications.

ANNEX III

GSPLAJ
AL-FATEH UNIVERSITY
VETERINARY MEDICINE COLLEGE

REF. W/O (23)DATE 12/11/1994

To: Dean, Veterinary college

Dear sir,

Reference is made to the MBRC letter dated 10/10/94 No. R&S/35/754/94 concerning the micro biological examination to some shrimp samples.

Please be informed that the direct microscopically inspection shows that samples are infected with:

Black Gill Disease
Fusarium Disease

Taking into consideration that the direct microscopically inspection is enough to diagnose this disease. Also, we would like to inform you that the number of samples was little and the pollution rate was very high for the samples and water thus the separation of the fungi in a clear way was difficult at the laboratory.

Moreover we would like to inform you that the cost of this test amounted to (50 L.D)

Regards.

Dr. Hussain M. Errais
Chief of Microbiology & Parasitology Section

cc. Section File.

ANNEX IV


Histopathological Report: Specimen No.22/94, presented by Mr. Ali Fituri, on 10/11/94. Several, intact formalized shrimps.(Penaeus japonicus)
Histopathology:
The most appreciable changes have been noticed in the skeletal muscles and characterized, primarily, by degenerative changes. The involved muscle fibres were undergoing disorganization and fragmentation due to accumulation of varying amounts of edema fluid . Some fibres were undergoing hyalinization and necrosis. The inflammatory response was minimal and presented by randomly scattered collections of inflammatory cells within the muscle fibres.
In the eye the crystalline tracts contain numerous vacuoles, some of which coalese together to form large empty spaces Similar, but less pronounced vacuolar pattern has been observed in the lamina ganglionaris
The changes in the rest of the organs were unremarkable. Selected PAS-stained sections failed to demonstrate specific Pathogens.
Pathologist:
 
 Osheik A. Ali
College of Veterinary Medicine,
Department of Pathology,
Al Fateh University - Tripoli.

LIBFISH TECHNICAL BRIEFING NOTES

01 Lamboeuf, M., A. Mujahid, & N. Elkebir. ‘Reconnaissance survey of the artisanal fishing fleet of Libya.’ TBN No. 1, April 1994. (En/Ar)

02 Bellec, O. ‘Drydocking and refit of RV NOUR and voyage of vessel to Valetta, Malta.’ TBN No. 2, April 1994. (En/Ar)

03 Meaden, G.J. ‘Background notes and prospects for a Libyan marine fisheries Geographical Information System.’ TBN No. 3, April 1994. (En)

04 Reynolds, J.E. & M. Lamboeuf. ‘1993 Libyan fisheries frame survey planning & preparation workshop.’ TBN No. 4, April 1994. (En)

05 Medina Pizzali, A,F. ‘Technical evaluation and recommendations for the Sabratah fish cannery.’ TBN No. 5, April 1994. (En/Ar - Limited Distribution)

06 Reynolds, J.E. & M. Lamboeuf. ‘1993 Libyan fisheries frame survey. Data handling/dBase management workshop.’ TBN No. 6, May 1994. (En)

07 Reynolds, J.E ‘Proceedings of the joint MBRC/FAO staff workshop on research training programme development (Tajura, 6 January 1994).’ TBN No. 7, May 1994. (En/Ar)

08 Lamboeuf, M. ‘Notes on the marine resources survey planning workshop (MBRC, Tajura, 31 January 1994).’ TBN No. 8, May 1994. (En/Ar)

09 Medina Pizzali, A.F. ‘Interim report on the national fish handling & processing facilities survey: Workshop proceedings (MBRC, Tajura, 26 March 1994).’ TBN No. 9, May 1994. (En/Ar)

10 Vallet, F. ‘Proposals for MBRC aquaculture facility rehabilitation.’ TBN No. 10, May 1994. (En)

11 Reynolds, J.E., M. Lamboeuf, A. Ben Abdallah, A. Abukhader, R. Abdulbari, & A. Nafati. ‘Preliminary findings of the 1993 Libyan marine fisheries frame survey: Workshop proceedings.’ TBN No. 11, June 1994. (En/Ar)

12 Magsoudi, M. & F. Vallet (eds.) ‘MEDRAP II Artemia Workshop (Libya) & Southern salt lakes mission.’ TBN No. 12, July 1994. (En/Ar)

13 Lamboeuf, M., A. Ben Abdallah, J. Sutherland, & E. Ansel. ‘Libya trawl survey: Preliminary results of the first coverage.’ TBN No. 13, July 1994. (En)

14 Lamboeuf, M. ‘Calibration of SIMRAD EK500 scientific sounder - RV NOUR .’ TBN No. 14, July 1994. (En)

15 Meaden, G.J. & J.E. Reynolds. ‘Establishing a marine fisheries GIS: the Libyan experience.’ TBN No. 15, Sept. 1994. (En)

16 Lamboeuf, M. & J.E. Reynolds. ‘The fishing fleet of Libya: preliminary results of the 1993 frame survey.’ TBN No. 16, Oct. 1994. (En)

17 Medina Pizzali, A.F., F. Vallet, & M. Magsoudi. ‘Proposal for an integrated small-scale solar salt and brine shrimp production unit.’ TBN No. 17, Oct. 1994. (En)

18 Vallet, F. ‘Ponds and raceways: Proposals for small-scale model fish farms.’ TBN No. 18, Nov. 1994. (En/Ar)

19 Fituri, A. & F. Vallet. ‘Investigation of Ain Kaam farm shrimp mortality: preliminary findings.’ TBN No. 19, Nov. 1994. (En/Ar - Limited Distribution).

LIBFISH HELD DOCUMENTS

01 Goanec, M. ‘RV NOUR 1993–1994: Chief Engineer's report.’ FD No. 1, June 1994. (En)

02 Reynolds, J.E., M. Lamboeuf, A. Ben Abdallah, A. Abukhader, R. Abdulbari, & A. Nafati. ‘Libyan coastal fisheries: A report on the 1993 baseline survey results.’ FD No. 2, July 1994. (En -- in prep.)



For further information please contact: Project Manager, LIBFISH
  
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 LIB/88/009 - GCP/LIB/021/lsDB Marine Biology Research Centre
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