Graft-transmissible diseases of citrus
-Handbook for detection and diagnosis of graft-transmissible diseases of citrus-
CORRIGENDUM
Page xiii
The address of Mr Steven M. Garnsey is now:
US Horticultural Research Laboratory
Agriculture Research Service
US Department of Agriculture
2120 Camden Road
Orlando, Florida 32803
United States of America
New publication data
M 14/T0601E/3.92/1500
ISBN 92-5-1031 82-7
C.N. Roistacher
Emeritus Plant Pathologist
University of California
Riverside, California, USA
INTERNATIONAL ORGANIZATION OF CITRUS VIROLOGISTS
FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS
Rome,1991
The designations employed and the presentation of material in this publication do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries.
All rights reserved. No part of this publication may be
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and extent of the reproduction, should be addressed to the
Director,
Publications Division,
Food and Agriculture Organizabon of the United Nations,
Viale e delle Terme di Caracalla,
00100 Rome, Italy.
© FAO, 1991
Foreword
Preface
Acknowledgements
Contributors
Abbreviations
Introduction
Part I.Techniques for biological detection of
specific citrus graft-transmissible diseases
Inoculation procedures for detection of citrus graft-transmissible pathogens (CGTPs)
Tristeza
Greening
Stubborn
Blight and related diseases
Exocortis
Cachexia
Satsuma dwarf
Tatterleaf
Infectious variegation and leaf rugose
Psorosis complex: psorosis-A, psorosis-B and ringspot
Concave gum
Impietratura
Cristacortis
Vein enation (woody gall)
Gummy bark and transmissible bud-union disorders
Part II.Facilities and techniques for biological detection of CGTPS
The plant laboratory
The greenhouse
Soil mixes for plant growth
Production and care of indicator plants
Techniques for graft-transmission in citrus
Part III.Laboratory methods for detection of CGTPs
Enzyme-linked immunosorbent assay (ELISA) for citrus pathogens
Detection of plant viruses and viroids by molecular hybridization
Viroid purification and characterization
Immunosorbent electron microscopy (ISEM) and antibody coating
Isolation and analysis of CTV dsRNA from citrus bark
Isolation and culture of Spiroplasma citri
Detection of citrus tristeza virus inclusion bodies using azure a staining and in situ immunofluorescence
Laboratory equipment needed for selected diagnostic procedures
Glossary