Virtual Training Course on Surveillance and Monitoring of Antimicrobial Resistance in Aquaculture
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The AMR action plan of FAO and One Health global strategy aim to foster wider awareness and develop the capacity for monitoring and surveillance of antimicrobial use (AMU) and AMR in aquaculture. Capacity for monitoring and surveillance is essential for the implementation of AMR national action plans. This training course is organized by FAO in collaboration with the Institute of Hydrobiology of Chinese Academy of Sciences (IHB-CAS), and INFOFISH. This is one of the efforts to enhance national capacity, through the support from an FAO regional technical cooperation programme: TCP/RAS/3702 Support Mitigation of Antimicrobial Resistance Risk Associated with Aquaculture in Asia Programme Day 1 (26 July 2021, Monday) | 14:00-17:00 Bangkok time (GMT+7) Session 1: Introduction and overview 14:00-14:20 Welcome remarks, introduction and virtual group photo 14:20-14:40 1.1 Status of AMU and AMR in Asia by Dr Aihua Li 14:40-15:00 1.2 Important bacterial diseases of aquatic animals by Dr Eduardo Leano 15:00-15:10 Coffee break
Session 2: Country reports on national surveillance of AMU and AMR 15:10-15:30 2.1 China report by Dr Hu Kun 15:30-15:50 2.2 India report by Dr Gaurav Rathore 15:50-16:10 2.3 Indonesia report by Dr Christina Retna Handayani 16:10-16:30 2.4 Thailand report by Dr Thitiporn Laoprasert 16:30-16:50 2.5 Viet Nam by Dr Le Thi Hue 16:50-17:00 Discussion and Q&A
Day 2 (27 July 2021, Tuesday) | 14:00-17:00 Bangkok time (GMT+7) 14:00-14:05 Introduction and virtual group photo
Session 3: Framework for AMR monitoring and surveillance 14:05-14:35 3.1 Global perspective on AMR monitoring and surveillance by Dr Junxia Song 14:35-15:05 3.2 Global perspective of AMR and AMU in aquaculture by Dr Melba Reantaso 15:05-15:15 Coffee break 15:15-15:45 3.3 International standards: CLSI and other laboratory standards by Dr Patricia S. Gaunt 15:45-16:15 3.4 Developing the framework of AMR surveillance in Asia by Dr Mary Gordoncillo 16:15-16:20 Wrap up
Day 3 (28 July 2021, Wednesday) | 14:00-17:00 Bangkok time (GMT+7) Session 4: Framework for sampling approaches by Dr Aihua Li 14:00-14:15 Pre-test 14:15-15:45 4.1 Purpose of AMR surveillance and monitoring 4.2 Overall purpose-based design for sampling 4.2.1 Prioritization of target aquatic animals and environment 4.2.2 Selection of aquaculture farms for sampling 4.2.3 Determining the timing/schedules for sample collection 4.2.4 Specimen numbering 4.2.5 Target bacterial species 4.3 Sample collection from aquatic animals and environment 4.3.1 Sample collection from live/moribund animals (whole animal or its tissue) 4.3.2 Environmental sample (water samples, sediment) 4.3.3 Transportation of various types of samples
15:45-15:55 Discussion and Q&A 15:55-16:05 Quiz and coffee break 16:05-16:50 4.4 Collection of sampling information 4.4.1 Clinical signs of sampled aquatic animals 4.4.2 Environmental parameters 4.4.3 Sample information recording 4.4.5 Other information 4.5 Preservation of bacterial isolates 16:50-17:00 Discussion, Q&A and virtual group photo
Day 4 (29 July 2021, Thursday) | 14:00-17:00 Bangkok time (GMT+7) Session 5: Sample processing and bacteria isolation by Dr Yuting Deng and Dr Lan Jiang 14:00-14:10 Recap of Day 3 14:10-16:00 5.1 Sample processing 5.1.1 Sample processing of tissue from aquatic animals 5.1.2 Sample processing of aquaculture environment
5.2 Procedures for isolation and identification 5.2.1 Procedures for isolation and identification of Aeromonas 5.2.2 Procedures for isolation and identification of Vibrio 5.2.3 Procedures for isolation and identification of E. coli
16:00-16:10 Discussion and Q&A 16:10-16:20 Quiz and coffee break 16:20-16:50 5.3 Preservation of the isolates 5.3.1 Choice of preservation 5.3.2 Short-term preservation 5.3.3 Long-term preservation 16:50-17:00 Discussion, Q&A and virtual group photo
Day 5 (30 July 2021, Friday) | 14:00-17:30 (GMT+7) Session 6: Methods for antimicrobial susceptibility testing (AST) by Dr Yuting Deng and Dr Lan Jiang 14:00-14:10 Recap of Day 4 14:10-15:10 6.1 General susceptibility considerations 6.1.1 Choice of methods for AST 6.1.2 Harmonized panel of antimicrobials for monitoring
6.2 Standardized susceptibility testing protocols 6.2.1 Protocol of disk diffusion method 6.2.2 Protocol of broth micro-dilution method
15:10-15:40 6.3 Interpretation of test results 6.3.1 CLSI reference for some monitoring procedures 6.3.2 Optional interpretive criteria for AMR surveillance 15:40-15:50 Discussion and Q&A 15:50-16:00 Coffee break 16:00-16:30 6.4 Quality control (QC) in AST 6.4.1 The goals of a QC programme for AST 6.4.2 Choice of reference strains 6.4.3 QC guidelines 6.4.4 Acceptable QC ranges
Session 7: Data management and reporting by Dr Yuting Deng 16:30-17:00 7.1 Data recording and storage: Regional template for antimicrobial resistance data collection 7.2 Data management and data analysis 7.2.1 Data management 7.2.2 Data analysis
17:00-17:15 Final exam 17:15-17:30 Closing remarks, evaluation and virtual group photo Facilitators
Registration: http://infofish.org/APFIC/index.php/amr-training
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