Potato yellow vein virus (PYVV) (Crinivirus/Closteroviridae) (Martelli et al., 2012), is an re-emerging plant virus (Salazar et al., 2000), spread in all Colombian potato producing areas. PYVV is transmitted by the greenhouse whitefly (Trialeurodes vaporariorum) in a semipersistent manner (Buritica, 1971). For the implementation of effective strategies for PYVV/T. vaporariorum control is necessary information about the relationship between the virus and its vector. In the present study PYVV transmission characteristics by T. vaporariorum: acquistion access period (AAP), retention period (RP) and inoculation access period (IAP) were evaluated. This is a new report related with PYVV/whitefly complex.