Table 4. Feed formulae (ingredient composition) and proximate composition of commonly used farm�made feed for Fenneropenaeus indicus and sample diets showing the feed formulation developments in other species
Ingredient/proximate composition (% dry matter)1,3 F. indicus F. indicus F. indicus F. indicus F. indicus F. indicus L. vannamei P. monodon F. indicus
Diet 1 Diet 2 Diet 3 Diet 4 Diet 5 Diet 6 Diet 7 Diet 8 Diet 9
Ingredient composition (%)                  
Fishmeal 22�35% 26.3 43.33b     28 6 5 26
Wheat flour 12�15% 27.2 29.3 21.5 21.5 10 15.9 8.4  
Soybean oil cake 25�33% 30 15.4 5 5 15 45.5 62 26
Squid oil 1�5%                
Brewer's yeast   5         2    
Yeast                 1
Squid liver powder 7�10%                
Squid meal 7�10%     0 1.5   2   5
Shrimp meal 10�15%                
Fish oil   2         4   3
Cod liver oil       1.75 1.75        
Fish solubles (dehydrated)       20 18.4        
Shrimp head meal 2�5%     13 13       5
Shrimp shell meal 9�10%         25      
Squilla meal                 5
Sunflower oil cake               2  
Tapioca powder                 19.49
Alfalfa                 1
Canola meal             19.5    
Wheat gluten   2.5              
Yellow corn     6.6            
Rice bran           10      
Groundnut oil cake           8      
Lactic yeast       6 6        
Blood meal       2 2        
Wheat gluten       15 15        
Soy lecithin (crude)   2   1.75 1.75   1 0.5 1
Cholesterol   0.2a         0.4 0.5  
Palm oil     2.6            
Mould inhibitor   yes         0.15    
Potato starch (Liagel)       3 3        
CaCO3       0.5 0.5        
NaH2PO4       3.5 3.5        
Mono calcium phosphate   2              
Vitamin C (Stay C)   0.4         0.1 0.1  
Vitamin & mineral mix   0.4 2.72     2 0.25 1  
Vitamin mix       3 3       1
Mineral mix   0.5         3   5.5
Choline chloride       0.75 0.75        
Binder     0.05            
CMC               1.5  
Agar           2      
Lysine   0.5b           1  
L�Methionine   12              
Cellulose       3.25 3.25        
BHA               0.01  
Proximate composition (%)                  
Crude protein 38�42 41.22 40 49.3 48.5   38.6 34.87 35
Crude lipid 40 063 5.95 4.5 6.9 6.9   8.8 11.55 6
Carbohydrate             36.4 44.43 20
Ash 12 6.45 12.5 9.5 9.2   8.2 9.2  
Crude fibre 3 4.12 2.2       3.5    
Moisture 11   12       8.4    
Calcium     1.8            
Phosphorus                  
Gross energy (MJ/kg feed)   17.75         15    
ME   12.11              
EPA+DHA (min) 1.2�1.4                
Phospholipid   >1.3   >1.14 >1.14   >0.65 >0.33 >0.65
Cost (US$/kg) 0.9 0.57              
FCR (harvest grams)   2.2 (22)              
Amino acid composition                  
Lysine   3.03   2.42 2.42   2.1    
Arginine   2.59   2.5 2.5   2.2    
Leucine   3.11   3.28 3.29        
Isoleucine   1.95   1.72 1.74        
Valine   2.38   2.11 2.11        
Threonine   1.51   1.62 1.64        
Phenylalanine   1.51   1.79 1.8        
Histidine   0.88   1.01 1.01        
Methionine   1.77              
Met + Cys             1.1    
Tyrosine   0.92              
Source King- Seahorse4 Sara, Gous and Bureau (2009) Salama (2000) Cruz- Ricque et al.  (1987) Cruz- Ricque et al. (1987) Amar, Philip & Bright Singh  (2006) Potential low FM diet, Su�rez et al. (2009)5 Potential low FM diet, Biswas et al. (2007)5 CIBA (1994)
Reported stocking density (per m2)   60   17 & 44 17 & 44        
13% cholesterol purity.
2There was no clearly evident benefit from the addition of these amino acids and their use was stopped in commercial operations.
3Abbreviations: %, percentage; CaCO3, calcium carbonate; NaH2PO4, sodium phosphate;MJ Megajoule; FCR, feed conversion rate/ratio; Met + Cys, methionine and cystine; EPA, Eicosapentaenoic; DHA, Docosahexaenoic; ME, metabolisable energy; BHA, Butylated hydroxyanisole (antioxidant); F. indicus, Fenneropenaeus indicus; L. vannamei, Litopenaeus vannamei.
4�King-seahorse� Brand Prawn Feeds, Sanfeng and Sanfapulin Feedstuffs Co., Ltd. , 1-601,9#, Rongtai Street, TEDA, Tianjin, P. R. China.  Zip Code: 300457; Tel: (0086-22)66283205, 66283201 66221030   Fax:(0086-22) 66283223   66221081;   
E-mail: [email protected], hanharryca@hotmail.com
5See also Davis et al. (2004) and Davis, Samocha and Bullis (2005)