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APPENDIX C
EQUIPMENT AND SUPPLIES TO BE PURCHASED

I. Water Chemistry and Soil Analysis Laboratory
QuantityDescriptionEstimated cost
(U.S.$)
   
1DO meter (DO + temperature; calibrated for salinity)1 200   
     1 setOxygen in situ recorder1 000   
     1 setBOD determination apparatus500
2Hach kits: for most water quality parameters with spare reagents1 000   
1
pH meter: photovolt, battery powered for field use, for AC/DC 110/125/220 v; 50/60 Hz
500
     1 setKjeldahl distilling apparatus, complete set3 600    
1Oven for temperatures up to 150–200°C375
1Still, 4 litres/hour, distilled water700
1Hood or suction manifold to exhaust fumes600
1Salinometer2 500   
36   BOD bottles, 300 ml capacity170
24   Nessler tubes, graduated at 50 and 100 ml120
               1 dozen/size
Desiccators, 220–300 mm diameter; rubber stops, one dozen of each size to fit each flask
  60
4  Reagent bottles, 16 oz or 500 ml  16
36   Dropping bottles, 60 ml polyethylene, or glass with pipette  25
25   Pipettes, ungraduated with rubber bulb (eye dropper)  26
6  Evaporating dishes, porcelain, 50 and 100 ml  19
12   Kjeldahl flasks (800 ml)350
12   Brown bottles, 16 oz or 500 ml, for reagent storage  20
500 g  Boiling beads    3
1Powder funnel 10–15 cm inside diameter         1.15
1Powder funnel 5–7.5 cm diameter         3.50
6Bunsen burners (if gas available)  70
1Magnetic stirrer  50
12   Magnetic stirrer bars (2–3 cm)    6
6Field thermometers, 0–50°C (4–6)120
2Thermometers, 0–200°C  18
2Sampling devices: bottle train samplers160
2Field reagent boxes: (lab)  20
1Rack for Nessler tubes  17
      1 boxWeighing paper, for use with analytic balance        1.50
1Rack for funnels  50
1Rack for Kjeldahl flasks  18
6Condensers, 300 mm  52
         3 boxesFilter paper, 15 cm Whatman no. 42  16
         3 boxesFilter paper, 15 cm Whatman no. 5  18
1 000       Labels    6
6Ring stands for burette support, 60–75 cm high  45
3Burette clamps, double  10
6Clamps, single, for general use  33
6Ring clamps or support rings or tripods, 125 m diameter  21
4Triangular files; glass cutter  12
12   Stoppers for Kjeldahl flasks    9
1Cork borer  11
 Sub-total13 552.15
   
Reagents for specific analyses
  
For DO determination 
24 kgH2SO4, concentrated, specific gravity 1.84 
500 g     Sulfamic acid (NH2SO2OH2) 
  2 kgMnSO4 
  5 kgNaOH 
  2 kgKI 
500 g     K2Cr2O7 
250 g     Soluble starch powder 
     2 litresGlycerine 
     8.1 kgHCl, concentrated, specific gravity 1.19 
  
For CO2 
10 g   Phenolphthalein indicator 
     2 litresEthyl or methyl alcohol, purified grade 
 1 kgNa2CO3, or 2 litres, 1-N standard NaOH 
   
Alkalinity  
10 g    Xylene cyanole indicator 
10 g    Methyl orange indicator 
   
pH 
          1 litre eachpH buffers, pH 5, 7, 9 
   
Ammonia 
    6 litresNessler's reagent or 500 g HgI2 
500 g       ZnSO4 
1 kgRochelle salt 
100 g       Lead acetate 
500 g       KH2PO4 
1 kgK2HPO4 
500 strips Litmus paper, red 
500 g        NH4Cl 
   
Hardness 
10 g      Calmagite (or chrome black T, Eriochrome Black T, or F.24) 
100 g        Hydroxylamine HCl 
250 g        CaCl2 
500 g        E.D.T.A. (Kersene) = Sodium salt of ethylenediaminetetracetic acid
100 g        MgCl2 
  1.8 kgNH4OH, specific gravity 0.90 
  
Kjeldahl digestion for organic nitrogen 
2 kgNa2SO4 or K2SO4 (Anhydrous) 
   
Salinity  
 0.1595 N silver nitrate 
 5 percent potassium chromate indicator or sodium fluorescinate (uranin) 
 Volumetric flask 
 125 ml Erlenmeyer flask 
 Magnetic stirrer 
  
Miscellaneous reagents 
2 kgAnhydrous CaCl2, granular, 4–8 mesh, technical grade 
1 kgSoda lime, granular, 4–8 mesh, technical grade 
 The total estimated cost of these reagents and supplies is1 900  
   
 Soil analysis 
1Oven (>100°C)250
1Mortar and pestle    5
1Sieve: 20-mesh screen  30
1pH meter (with glass electrode)475
1Mechanical stirrer  75
1Oven (at least 80°C)125
1Hot plate  45
  
Pond mud analysis 
 Ammonia nitrogen 
 1N H2SO4 
 K2SO4 
 NaOH 
 HCl 
 Antifoam: paraffin oil + octyl-alcohol 
 Volumetric flask 
 Nessler's reagent 
 Standard NH4 solution 
 Beaker, volumetric flask 
 Buchner funnel with numbered filter 
 Ammonium acetate: pH 7.00 
 Ammonium hydroxide 
 Acetic acid 
 HCl 
 HNO3 
 Hydrogen peroxide (30 percent analytical grade) 
 NaOH solution 
 Murexide indicator 
 Aqua-Regia 
 EDTA 
 Devanda's alloy metal: 49.4 percent Cu, 45.5 percent Al, 4.7 percent Zn
 Sodium sulphate - mercuric oxide 
 Zinc metal (granulated zinc) 
 Buffer pH - 8.00, p-nitrophenol 
 Boric acid 
 KOH pellets 
 KCl 
 Estimated total cost of equipment and supplies1 925  
 Sub-total17 377    
 
II. Fish Nutrition Laboratory
   
1Pellet press500
1Pelleting machine with 1/8-inch diameter ring4 000   
1Precision balance (analytical) capacity 160 g2 000   
1Feed mixer, lab model1 500    
   1-11/8-inch and 3/32-inch food grinder plate1 970    
1
Hammer mill: 66 hammers, 15 x 24-inch diameter chamber with 1/8-inch and 3/32-inch grinder plate; screens with 15 hp electric motor
4 000   
1Hobart mixer 12 qt1 000   
1Grinding head with an 1/8-inch extruding plate  50
1Spectrophotometer2 560   
1Bomb colorimeter350
1Goldfish fat extractor, di-electric, 6-position1 600   
1Spectronic 20 colorimeter with line voltage regulator, moisture-proof model900 
1Stereoscopic microscope1 900   
1Compound microscope with illumination450
1Stage or ocular micrometer, counting chamber, or grid-marked petriplate  95 
1Automatic shaker  80
1Centrifuge325
1Kjeldahl distilling apparatus (digestion and distilling); 12-position3 600   
1Digestion rack  37
1Refluxing apparatus  68
1Rheostat  56
1Fume hood and blower for penchloric acid digestion1 900    
1Waring blender  70
     1 setSieves, ASTM standard sizes no. 10 to no. 80175
1Food grinder  45
     1 setFibre filter with six metal screens100
1Magnetic stirrer  75
1Water bath, thermostatic, 26 × 15 × 16 inches  65
1Hot plate, electric, thermostatic, 12 × 13 inches  65
6Thermometers184
1Micro-distillation apparatus  20
        2 pairsAsbestos gloves  12
8Metal applicators   3
8Scrapers   5
3Aspirators, 7-inch  67
1Titration lamp  60
1Suction pump (and bottle)  16
-Bench clamps  12
1Fish deboner (separator)12 000    
1Fish smoking machine7 000   
1High sensitivity balance1 500   
1Balance, top loading (2-kg capacity)550
1Scales, 200 kg650
1
Muffle furnace with temperature controls and pyrometer, 5 ½ × 15 × 4 ½ inches
670
1Oven (>100°C)365
     1 setColumn thin chromatography, plates, mounting boards, plus accessories27 500     
1Refrigerator/freezer1 640    
1Storage freezer, chest type, 17.2 ft31 400    
   50
Glass aquaria, capacity 76 litres each, with supports, plumbing, valves, pumps, compressors
8 000    
   20
Tanks, fibreglass, 210-litre capacity each with standpipe plus supports, plumbing, valves, pumps, compressors
7 500    
2Seines and seining equipment/materials675 
1Forced air-drying oven, 36 × 60 inches, 45 ft3, electric3 900    
1Water still, electrically heated, 8 litres/hour capacity400
1DO meter + thermometer (calibrated for salinity)1 200    
1
pH meter, AC battery operated crude fibre analyser, 6 positions, laboratory model; two spare electrodes
550
  12Portable aerators: 12 volts, DC/AC2 800   
1
Water pump with pressurized storage tanks to deliver 900 gal/min at 20 ft elevation (3 407 litres/min at 6.09 m)
3 560    
1
Air compressor (oil-free) to 165 lb/in2, 20-gallon tank or blower, Sutombilt 5 hp
575
2Air pumps (Conde type)750
1Oxygen tank valve (gauge)  76
   
Hardware 
  12Wire gauzes, 15 × 15 cm; asbestos centre  60
2Crucible tongs  14
  
Volumetric flasks 
  101 litre flasks  80
  10500 ml flasks  76
  10200–250 ml flasks  69
  
Volumetric pipettes 
   3–45 ml pipettes  60
   3–410 ml pipettes  45
   1–225 ml pipettes  16
   1–250 ml pipettes  18
   3–4100 ml pipettes  34
   1–2200 ml pipettes  16
  
Graduated pipettes 
51 ml, in hundredths of ml  15
52 ml, in tenths of ml  16
55 ml, in tenths of ml  16
510 ml, in tenths of ml  20
  
Graduated cylinders 
250 ml    8
   3–4100 ml  27
   3–4200 ml  28
   3–41 000 ml  56
   
Burettes 
250 ml, in tenths of ml  70
225 ml, in tenths of ml  65
110 ml, in tenths of ml; squeeze bottle, self-zeroing115
  
Beakers 
   5–6100 ml, griffin low form with lip  22
   5–6250 ml             "  19
   3–4600 ml             "  21
   3–41 000 ml          "  30
  
Flasks (Erlenmeyer, narrow-mouth) 
12   125 ml  50
24   250 ml  85
12   500 ml  65
12   1 000 ml  66
  
Miscellaneous glassware 
24   Funnels, 5–7.5 cm, long stem  36
 30 mRubber tubing, 5–6 mm inside diameter  54
 10 mGlass tubing, 5–6 mm diameter105
1Storage jar for distilled water; 40–50 litres capacity  50
6Wash bottles, 500 ml polyethylene    7
1Desiccator, with stopcock covers, 250 mm inside diameter100
1Ashing crucible + lid (dried and tared)  15
 Crucibles (for drying on drying pan) 
 Dropper and bottle 
 Tared porous crucibles 
 Microlitre pipettes 
 Curvets 
 Micro-rod (made by flaming glass rod and drawing out) 
 Mortar and pestle 
 Slides (microscope) 
 Paraffin oil 
 80 percent acetic acid 
 Nitric acid (concentrated) 
 Benzene 
 Purified benzoic acid 
 0.0725 Na2CO3 
 Standard CaCO3 
 EDTA solution 
 Ca indicator - Murexide 
 Molybdavanadate 
 Ammonium molybdate tetrahydrate 
 Ammonium meta-vanadate 
 Perchloric acid 
 K2H2PO4 
 9N H2SO4 
 2 percent 2.4 dinitrophenylhydrazine 
 10 percent metaphosphoric acid 
 Bromine 
 Thiourea 
 Ethyl acetate 
 3 percent acetic acid in methylene chloride 
 2 percent acetic acid in ethyl acetate 
 Silica gel 
 Methyl chloride 
 Nitric acid 
 Sel-dahl pack (premixed catalysts) 
 H2SO4, concentrated 
 NaOH (saturated), pelleted 
 Boric acid, 4 percent 
 Ethanol, 95 percent 
 Standardized 0.1N HCl 
 Sodium lauryl sulfate, USP 
 Disodium dihydrogen ethylene-diaminetetracetic dihydrate, reagent grade 
 Disodium hydrogen phosphate, anhydrous, reagent grade 
 2-ethyl ethanol (ethylene glycol, monoethyl ether), purified grade 
 Decahydronaphthalene 
 Acetone 
 Sodium sulfite 
 1.25 percent H2SO4 
 1.25 percent NaOH 
 Reagent grade ethanol 
 Indicators: bromocresol green, methyl red 
 
Antifoam compound (Dow corning antifoam A diluted 1 and 3 with petrol, ether or water)
 
 Methylene chloride 
 Silica gel-G 
 Dispersion I, chloral hydrate, glycerine 
 Dispersion II 
 15 X, 45° and blue filter for stereoscope 
 Dextrin 
 Casein (vitamin-free or not) 
 Soy protein isolate 
 Blood fibrin or protein sources 
 Cellulose 
 Complete vitamin mixture 
 Vegetable oil 
 Cod liver oil 
 Complete mineral mixture 
 Carboxymethyl-cellulose (binder) 
 
Drugs, terramycin, nitrofurazone, potassium permanganate, formalin, malachite green, quinaldine, acriflavine, tetracycline
 
 Gelatin 
 Carboxymethyl-cellulose (CMC) 
 Anti-oxidant 
 Calcium propionate 
 Fertilizer (organic/inorganic) 
 Activating material for filters 
 Filter paper 
 
Wire baskets (water stability tests of fish feeds), 3 mm2 wire mesh, ca 5 cm square and 1 cm deep
 
 Foil 
 Aluminium foil 
 Plastic bags - various sizes 
 
Miscellaneous materials (air stores, tubing, hoses, cleaning equipment, containers, etc.)
 
   
 The total cost of those items for which individual prices have not been cited is estimated at7 650    
 Sub-total123 003       
 
III. Disease Laboratory
For bacteriological research 
1
Microscope + case; compound, phase contrast with triocular head (light/dark field objectives)
2 800   
1Microscope + case; dissecting-cycloptic with camera adapter 10X and 15X oculars1 600   
1Microtome; plus extra blades, paraffin, etc.1 500   
1Spectronic - 20 colorimeter900
1Magnetic stirring apparatus with rods110
1Incubator 30–50°C900
2Incubators 0–50°C1 700   
1Autoclave, portable1 500   
1Oven, sterilizing750
1Hot plate  45
1Centrifuge, microhematocrit525
1Torsion prescription balance600
1Triple-beam balance  80
1Demineralizer for purifying water225
1Glass distillation apparatus700
1Refrigerator-freezer (17.2 ft2)750
1Heating table for slides plus paraffin oven500
  
For viral research 
1Inverted TC microscope1 700   
1Sterile hood, laminar flow2 000   
1Vacuum pump190
1Cornwall continuous syringe (2.5 ml, 10 ml)200
1Filter, large capacity millipore with pressure vessel650
24   Swinny adapter filters560
-Millipore filters of assorted porosity200
  
For parasites research 
1Camera, Lucida130
1Centrifuge, clinical400
1Timer  20
1pH meter (lab/field)475
-Seines, dipnets, etc. for collecting fish1 500    
2Lamps, gooseneck desk lamps 65
1Dissecting kit; forceps, scalpels, scissors, droppers, probes, needles, bone shears  75 
     12 eachDissecting pans, specimen dishes, petri dishes, embryo and culture dishes140
2Bunsen burners (touch-o-matic)  70
6Bacteriological inoculating loops  49
5
Troughs; with running water (210-litre trough) plus supports, plumbing valves, pumps, compressors and valves)
 4 000   
60   
Aquaria; 76 litres each (about 50 with air and water lines) plus supports, plumbing valves, pumps, compressors and valves
 9 600   
1Pipette washing apparatus125
6Mortars + pestles  30
-Disposable syringes (2.5, 5 and 10 ml)100
-Microscope slides, cover slips, labels, slide boxes, etc.400
 Pipettes (1.5 and 10 ml)200
 Bacteriological glassware (tubes, petri dishes)400
 Glassware, beakers, flasks, cylinders700
 Pipette cans  70
 Ruber tubing and stoppers of various sizes150
 
Bacteriological media: TSA, BI agar (brain heart infusion)
- plates, Pseud-Fagar, Sulphide, Bacto-Furunculosis-Agar
- 25°C, Dextrose broth, Ordals media
 200
 Bacteriological stains150
 Antibiotics: acriflavine, terramycin400
 Reference books, specimens, etc.700
 Drawing material (paper, ink, pens and pencils, etc.)250
 Potassium permanganate200
 Liqui-nox detergent  40
 Alcohol (ETOH) 
 Permount 
 Xylene 
 Formalin solution 
 Bouins (fixing) 
 Lugols 
 Glycerine jelly 
 Stains (Semichon's stain), carmine, hemitoxylin, fast green 
 Acetone 
 Acids: HCl, H2SO4, glacial acetic, NaOH 
 Malachite green 
 Dylox 
 BHC (Benzene hexochloride): wettable powder 
 Chlorotone 
 Cytochrome oxiadase test (with “Patho-Tec CO” test paper) 
 The total estimated cost of the last 15 items is2 500   
 Sub-total43 824     
 
IV. Limnology Laboratory
1
Compound microscope, with sub-stage condenser; objectives - 16 mm, 4 mm, oil immersion, and 10 ocular. Mechanical stage (1)
 1 800    
1Stage micrometer, 2 mm × 0.1 and 0.01 mm  32
1Ocular micrometer disc, 5 mm in 0.0 mm  45
2Counting cells, Sedgwick-Rafter, plankton  36
1Stereoscopic microscope 10X – 70X1 900    
1Ocular micrometer disc, whipple  45
1Thermograph + charts + ink; winding type140
1Nephelometer, Coleman model 91 100   
1Plankton centrifuge480
1Turbidimeter375
1Stop watch  35
1Kemmerel sampler with messenger400
2
Van Dorn water samplers with messenger, with transport cylinder for observing stratification of algae (to be operated on a cable)
320
1Ekman dredge or modern one175
1Current meter1 000    
1Triple beam with weights to 2 000 g capacity, with 0.1 g divisions100
1Dial-o-gram, 311 g by 0.01 g100
1Analytical balance, sensitive to 0.1 mg2 000    
2Transits with tripods, plus book with tables2 400    
2Alidades170
2Planetables, to measure areas up to 5 ha150
1Steel tape, 50 m in length  17
2Protractors   8
1Stadia rod  85
1Polar planimeter  75
1Compass  30
1Hand lens    4
2Flags: 1 white, 1 red  20
-Stakes + nails (numbered)    8
14   Clip boards    4
-Dye118
-Materials available for preparing maps, plans, blueprints350
1Oven, drying500
1Furnace, muffle 1 400°F700
1Plankton net with detachable bucket, no. 20 bolting cloth130
1Extra bucket for above110
1Sieve for screening (30 mesh/inch)  25
2Secchi disks  36
1Graduated line for depth measurements   5
   2 mExtra bolting cloth no. 20 for replacement; at least 2 m  60
15   Thermometers (max-min; °C or °F)285
2Electric resistance thermometers, 15 m300
1Hensen-Stemple pipette 1 × 2 ml  60
2Dessicators100
24   BOD bottles (including 2 train apparatus)100
2File cabinets (for charts, maps, plans, etc.)300
1Drafting table300
 Sub-total16 535     
 
V. Miscellaneous Equipment and Materials
2Desk calculators4 500    
2Portable calculators (with adaptor and recharger)750
1Photocopier, with supplies; annual lease for use of machine7 500    
12   Pocket thermometers (50°C)  72
24   Portable aerators 110/220 v, 50–60 Hz2 400    
8Battery operated (DC) aerators1 200    
2Weighing scales (balances) with weighing rig for large weights (sampling, harvesting)1 000   
2Smaller weights (feed allowance, individual weights L-W relationships, etc.)970
3
Boats (variable sizes, from 4–5 m; oars, locks, anchor + lines, tools, spare parts, trailers, life jackets, fuel tanks, truck hitch)
12 000     
4Outboard motors7 000   
3Fish transporters (tanks, portable)2 200   
2Winches (hauling large seines)2 000   
2Seines - 5 m (with 1,6 mm, sq. mesh)  80
1Seine  - 8 m (3.2 mm, sq. mesh)115
1    "     16 m (12.7 mm, sq. mesh)180
1    "     32 m (12.7 mm, sq. mesh)475
1    "     32 m (6.4 mm, sq. mesh)510
1    "     61 m (25.4 mm, sq. mesh)890
1    "     61 m (50.8 mm, sq. mesh)830
1    "   183 m (50.8 mm, sq. mesh)1 500   
1    "   183 m (63.5 mm, sq. mesh)1 200   
24   Hand nets288
1Machine bag sealer (seal bag)200
12   Metersticks  48
2
Smooth tables for processing fish (counting, measuring, weighing) 1.94 × 1.0 × 0.80 m
665
50   Vials (100 IU each) of human chorionic gonatropine hormone (HCGH) 650
 
Lines, other nets, string, ropes, nylon lines, buckets, tubs, baskets, ice boxes, plastic bags (several sizes)
 4 492   
 Rotenone1 775   
 Quinaldine140
 Feeds plus raw ingredients9 000   
 Fire extinguishers400
 Sub-total66 830      
 
VI. Feed Mill
8Storage bins (outdoor)2 500    
1Grinder-sifter4 400    
1Mixer3 400    
1Pellet mill14 000      
1Pellet dryer-cooler4 800    
1Crumbler-sifter2 600    
1Sifter no. 12 600    
1Sifter no. 23 600    
 Sub-total37 900     
 
VII. Vehicles
4Pick-up trucks - double cabin, 4-wheel drive; traction, winch, etc.24 000     
1Station wagon, 4-door standard or auto8 000    
1Minibus, capacity 30 passengers20 000      
2Jeep-like vehicles, 4-wheel drive and winch8 500    
 Sub-total60 500      
 
VIII. Audiovisual Laboratory
1
Movie projector, Super-8 mm, with sound, accessories, spare bulbs, reels, extensions, spare parts
650
1
Movie projector, 16 mm, with sound, accessories, spare bulbs, reels, extensions, spare parts
 1 870    
2
Slide projectors, 35 mm, synchronizer sound/recorder, other accessories, slide trays, slide boxes or files
 1 175    
2
Tape recorders, portable, cassette type, with accessories - charger, adaptor, AC/DC operated (115/220 v); tapes
 500
 Dark room equipment, supplies and materials3 225    
 Editing and splicing equipment and supplies725
 
Filming materials and supplies - movies, slides (Super-8 mm, 16 mm, 35 mm) and still photography; for developing, processing, etc.
 2 000    
 Screens (2–3) portable, pointers (2), stickup and bulletin board, stand180
 Sub-total10 325      
 
IX. Library
 Shelves: to store books, journals, reprints, etc. 
 Cabinet (with shelves): to store maps, charts, blueprints, etc. 
 Card files 
 Filing cards (size: 76.2 × 127 mm) 
 Office supplies 
 Display stands and tables 
 Desks (3) plus chairs (3) 
 Three study and reading tables (4 m2 each) 
 Bulletin board 
 Chairs (24) 
 Typewriter (1) 
 Sub-total4 579    
 
X. Classroom
1Desk for instructor175
1Chair (instructor)  17
40   Desks for students and visitors2 400   
40   Chairs400
1Podium with microphone and light200
   1-1Display shelves (stand) and table  80
2Wall boards100
1Wall screen, portable  29
 
Maps of world, continents, specific areas, regions or countries, with stand or mount on which these may be displayed
 350
 Classroom supplies - erasers, pointer, adhesive board (portable), chalk130
 Sub-total3 881  
 TOTAL384 754       

Figure 2 The proposed lay-out of experimental ponds at Pirassununga

Figure 3 Monthly mean water temperature (°C) in Pirassununga Recorded approx, 12.00 H, Depth - 20 cm

Figure 3

Figure 4 Cross-sections of proposed ponds at Pirassununga (cross-sections a-a', b-b')

Figure 5

Figure 5 Floor plan of the proposed hatchery in Pirassununga


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