Alfalfa Line KK179 (MON00179-5) has reduced levels of lignin by the introduction of a partial caffeoyl CoA 3-O-methyltransferase (CCOMT) gene sequence derived from Alfalfa. The gene transcript acts, via suppression of the endogenous CCOMT gene, to reduce the lignin level. Explants of the lucerne line ‘R2336’ were transformed via Agrobacterium-mediated transformation, the genes of interest being inserted via two separate T-DNAs. T-DNA I contains two CCOMT fragments, that when transcribed lead to the production of double-stranded RNAs (dsRNAs) that, via an RNA interference (RNAi) mechanism, suppress endogenous CCOMT RNA levels, leading to reduced biosynthesis of G-lignin. A T-DNA II was also inserted during transformation. This contained a neomycin phosphotransferase II (nptII) coding region that confers resistance to kanamycin. T-DNA II was removed Alfalfa Line KK179 has reduced levels of lignin by the introduction of a partial caffeoyl CoA 3-O-methyltransferase (CCOMT) gene sequence derived from Alfalfa. The gene transcript acts, via suppression of the endogenous CCOMT gene, to reduce the lignin level. Explants of the lucerne line ‘R2336’ were transformed via Agrobacterium-mediated transformation, the genes of interest being inserted via two separate T-DNAs. T-DNA I contains two CCOMT fragments, that when transcribed lead to the production of double-stranded RNAs (dsRNAs) that, via an RNA interference (RNAi) mechanism, suppress endogenous CCOMT RNA levels, leading to reduced biosynthesis of G-lignin. A T-DNA II was also inserted during transformation. This contained a neomycin phosphotransferase II (nptII) coding region that confers resistance to kanamycin. T-DNA II was removed from KK179 by selection. Molecular analyses of KK179 indicate there is a single insertion site at which there is a single copy of the T-DNA I. No DNA sequences from T-DNA II or from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred to the plant. The introduced genetic elements are stably inherited from one generation to the next. No potential public health and safety concerns have been identified in the assessment of KK179. Food derived from KK179 is considered to be as safe for human consumption as food derived from conventional alfalfa. from KK179 by selection. Molecular analyses indicate there is a single insertion site at which there is a single copy of the T-DNA I. No DNA sequences from T-DNA II or from the backbone of the transformation vector, including antibiotic resistance marker genes, were transferred to the plant. The introduced genetic elements are stably inherited from one generation to the next. No potential public health and safety concerns have been identified in the assessment. Food derived from the transgenic alfalfa is considered to be as safe as food derived from conventional alfalfa.