1. Allen-Nelson “Miquel seawater”
| Solution A | ||
| KNO3 | 20.2 g/100 ml.H20 | |
| Solution B | ||
| Na2HP04.12H20 | 4.0 g | |
| Cacl2.6H20 | 4.0 g | |
| HCl conc. | 2.0 g | |
| FeCl3 (melted) | 2.0 ml | |
| Distilled water | 80.0 ml | |
Add 2 ml. solution A to 1 liter seawater and 1 ml. of solution B to 1 liter of seawater.
II. Conwy Medium (Walne, 1974)
| A. | Sodium Nitrate | 100 g |
| EDTA, disodium salt | 45 g | |
| Boric Acid | 33.6 g | |
| Sodium Phosphate, monobasic | 20 g | |
| Ferric Chloride, 6-hydrate | 1.3 g | |
| Manganous Chloride, 4-hydrate | 0.36 g | |
| Trace Metal Solution* | 1 ml | |
| Vitamin Mix** | 100 ml | |
| Distilled water (to make) | 1000 ml | |
| (Note: use 1 ml Conwy medium/liter of seawater) * Trace Metal Stock Solution | ||
| Zinc Chloride | 2.1 g | |
| Cobalt Chloride, 6-hydrate | 2.1 g | |
| Ammonium Molybdate, 4-hydrate | 2.1 g | |
| Copper Sulfate, | 2.0 g | |
| Distilled Water | 100 ml. | |
| (Note: acidify with IN HCl until solution is clear) ** Vitamin Mix | ||
| Vitamin B12 | 10.0 mg | |
| Vitamin B1 | 20.0 mg | |
| Distilled water | 200 ml | |
III. TMRL Enrichment (Liao & Huang, 1970)
| KNO3 | 100 g |
| Na2HPO4.12H20 | 10 g |
| FeCl3.6H20 | 3 g |
| Na2SiO3.9H20 | 1 g |
| Distilled water | 1000 ml |
| (Note: Use 1 ml TMRL/liter of seawater) | |
IV. Enrichment for Outdoor Cultures
| 16–20–0 | 12 g |
| Urea 46 | 12 g |
| 21–0–0 | 100 g |
| (Note: add above nutrients to 1 ton seawater) | |
V. Modified F Medium (modified from Guillard & Ryther, 1962)
| A. | N-P Stock (500x) | |
| Sodium nitrate | 42.07 g | |
| Sodium Phosphate, monobasic | 5.00 g | |
| Distilled water (to make) | 1 liter | |
| B. | Sodium Metasilicate Stock (500x) | |
| Sodium metasilicate | 15.0 g | |
| Distilled water (to make) | 1 liter | |
| C. | Ferric Chloride Stock (500x) | 1.45 g |
| Distilled water (to make) | 1 liter | |
| D. | EDTA Stock (1000x) | |
| EDTA, Disodium salt | 10.00 g | |
| Distilled water (to make) | 1 liter | |
| E. | Vitamin stock (1000x) | |
| Thiamine HCl | 0.2 g | |
| B12 | 10.0 ml* | |
| Biotin | 10.0 ml** | |
| * B12 stock (primary) | ||
| -B12 | 0.1 g | |
| Distilled | ||
| water 1 liter | ||
| ** Biotin primary stock | ||
| Biotin | 0.1 g | |
| Distilled water | 1 liter | |
| F. | Trace Metal Stock (1000x) | |
| TM primary stock A | 1 ml | |
| TM primary stock B | 1 ml | |
| TM primary stock C | 1 ml | |
| TM primary stock D | 1 ml | |
| Distilled water (to make) | 1 liter | |
| 1) TM primary stock A solution | ||
| Copper sulphate, 5-hydrate | 1.96 g | |
| Zinc sulphate, 7-hydrate | 4.40 g | |
| Distilled water (to make) | 100 ml | |
| 2) TM primary stock B solution | ||
| Sodium molybdate, 2-hydrate | 1.26 g | |
| or | ||
| (Ammonium molybdate, 4-hydrate) | 0.907 g | |
| Distilled water (to make) | 100 ml | |
| 3) TM primary stock C solution | ||
| Manganous Chloride | 36.00 g | |
| Distilled water (to make) | 100 ml | |
| 4) TM primary stock D solution | ||
| Cobalt Chloride | 2.00 g | |
| Distilled water (to make) | 100 ml | |
NOTE: Dispense 2 ml each of solutions A, B, & C/liter of seawater
Dispense 1 ml each of solutions D, E, & F/liter of seawater.
