FAO ANIMAL PRODUCTION AND HEALTH PAPER 84
Training manual for embryo transfer in water-buffaloes
Cover photo: The world's first buffalo Calf born by embryo transfer on 18 March 1983 at the University of Florida and his recipient dam (Photo courtesy of UF Health Science Center Communications)
College of Veterinary Medicine
University of Florida
Gainesville, Florida, USA
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This manual is intended as a user's guide for the collection and transfer of buffalo embryos. Most of the techniques are based on the author's own experience and were adapted from the same techniques in cattle. Where necessary, specific differences between buffaloes and cattle are pointed out.
For the advanced procedures of cryopreservation, embryo splitting and embryo sexing, the reader is referred to the FAO training manual for bovine embryo transfer, by Dr George E. Seidel, Jr., to which this manual serves as a supplement.
Individuals with experience in buffalo breeding and with a basic understanding of reproductive physiology as well as reproductive endocrinology, and who possess reasonable artificial insemination and rectal palpation skills, should be able to master the techniques of embryo collection and transfer by following the procedures out-lined in this manual. Exercises to perfect such skills are provided in Recommended exercises to master embryo transfer skills.
FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS
Rome, © FAO 1991
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PHYSIOLOGY AND ENDOCRINOLOGY OF THE OESTROUS CYCLE
OESTRUS DETECTION AND INSEMINATION
NON-SURGICAL EMBRYO RECOVERY
FLUSHING AND HOLDING MEDIA
EMBRYO HANDLING AND EVALUATION
RECIPIENT SELECTION AND SYNCHRONIZATION
DONOR AND RECIPIENT AFTER-CARE
RECOMMENDED EXERCISES TO MASTER EMBRYO TRANSFER SKILLS
Sources of information
Comparison of embryo transfer results between buffaloes and cattle
Superovulation treatments with follicle-stimulating hormone in the buffalo
Superovulation treatment with pregnant mare serum gonadotrophin in the buffalo
Modified Dulbecco's phosphate-buffered saline solution
Effect of embryo quality on the pregnancy rate in cattle
Schematic representation of the oestrous cycle
Equipment for non-surgical embryo recovery
Location of the balloon of the recovery catheter at the base of the uterine horn
Diagrams of normal, excellent quality embryos at different stages of development
Diagrams of good quality embryos
Diagrams of fair quality embryos
Diagrams of poor and very poor quality embryos
Equipment for embryo searching
Searching grid-bottomed dish under stereomicroscope
Equipment for non-surgical embryo transfer
Location of the tip of the transfer syringe in the ipsilateral uterine horn at the time of embryo deposition
The author is indebted to Mr A.P. Leonards and Dr W.S. Cripe, North American pioneers in the field of water-buffalo production, for their generous support and constant encouragement.
He also wishes to thank Ms N.J. Drost for the figures, Ms B. Smerage for the typing of the manuscript and Dr Hock Seng Tan of the Universiti Pertanian (University of Agriculture) Malaysia for reviewing the manuscript.