(Supplies are listed in chronological
order.)
To select donors and recipients:
- palpation sleeves
- lubricant
- blood tubes and needles (blood
typing)
To treat donors and recipients:
- prostaglandins (PG)
- follicle-stimulating hormone
(FSH)
- pregnant mare serum
gonadotrophin
- 1-ml syringes (FSH) + 1.5-in
20-gauge needles
- 5-ml syringes (PG) + 1.5-in
18-gauge needles
To recover embryos:
- 2 percent lidocaine
- cervical dilator
- 2-way Foley catheter with
30-ml balloon: 18 and 20
French gauge most commonly
used (1 French gauge = 0.3 mm)
- 20-ml syringe to inflate balloon
- stylet, stainless steel: 0.5-ml AI
syringe plunger, 0.25-ml AI
syringe plunger (for 16-gauge
Foley) or stainless steel welding
rod
- tubing, clear preferably
siliconized: ID 6.4 mm, wall
1.6 mm, OD 8.0 mm
- Y-connector: OD 6.4 mm
- clamp, to fit tubing
- embryo filter, 75-μ pore size
- 1 000-ml graduated glass
cylinder
- Dulbecco's phosphate buffered
saline
- heat inactivated serum
- bovine serum albumin (BSA)
- antibiotic-antimycotic agents
To handle and evaluate embryos:
- fine tubing, ID 1 to 2 mm, for
siphoning
- 30–35-ml syringe with 22-gauge
needle, to clear filter
- searching dishes with grid,
100 x 100 x 15 mm square
- holding dishes, 35 x 10 mm
round
- acrodisc filter 22 or 45 μ,
sterile to filter holding medium
- stereomicroscope with 10X or
15X eyepieces, magnification
10–70X
- optional: compound
microscope, preferably inverted
with 100X and 200X
magnification
- light source for microscope
- extra light bulbs for microscope
- slide warmer (warming tray) for
holding dishes during
temporary storage
- catheter tips or Tomcat catheters
To transfer embryos:
- 0.25-ml clear semen straws
- 1-ml syringe
- transfer syringe (stainless steel):
regular Cassou AI syringe,
special transfer syringe
- protective sheath for transfer
syringe
- sterile, plastic sanitary sleeve
- routine surgical supplies for
laparotomy
