Agroecology is receiving increasing attention and recognition as a concept for transitions to more sustainable agricultural and food systems. There is however a lack of characterization of agroecology in agricultural and food systems, while integrated and holistic measurements of their sustainability are scarce. Community Supported Agriculture (CSA) is considered to be a system explicitly based on agroecological principles and practices which shows potential in the face of the sustainability challenges in agriculture and food systems, but its link with agroecology and its holistic sustainability performance have remained understudied. Therefore, this study applied the Tool for Agroecology Performance Evaluation (TAPE) to 24 Community Supported Agriculture farms in the Flanders region of Belgium in order to characterize agroecology and to assess their multidimensional sustainability performance.