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African swine fever: detection and diagnosis – A manual for veterinarians












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    Project
    Strengthening Biosecurity Preparedness through Enhanced Rapid Detection of African Swine Fever in Papua New Guinea - TCP/PNG/3706 2021
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    African Swine Fever ( is a highly contagious and fatal hemorrhagic viral disease that affects susceptible Suidae family, including pigs and wild boars In 2019 an expert team from the Emergency Management Centre for Animal Health (EMC AH) FAO conducted a Rapid Preparedness Assessment for ASF in Papua New Guinea, and concluded that the country was on high alert for an imminent incursion Recommendations were given to the National Agriculture Quarantine and Inspection Authority ( the country’s mandated Biosecurity and Veterinary service, on active reporting and surveillance, early detection, control and containment of ASF in the event of an incursion The Mission team, in collaboration with the NAQIA, the Department of Agriculture and Livestock (DAL and other relevant agencies and stakeholders, identified high risk entry pathways for ASF, namely the Indonesia and Papua New Guinea land borders, airports and seaports, through mining, logging and construction sites with Chinese or Asian contractors and/or workers The early detection of ASF was pertinent in the efficient and timely control and containment of the disease Given the high socio economic value of pigs in the country, particularly in the Highlands Region, it was imperative that an incursion be prevented and that ASF be kept out of this region Against this background, the project was designed to address gaps identified in the existing animal surveillance system in the NAQIA, and to strengthen the technical capacity of the NAQIA, the DAL and relevant agencies’ officers in active surveillance and reporting, diagnostics, and risk communication, to enable rapid detection and early containment of ASF incursion in the country.
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    Brochure, flyer, fact-sheet
    African swine fever
    You can stop ASF spread
    2022
    This leaflet encourages veterinarians to recognize the symptoms of African swine fever (ASF), a deadly disease affecting pigs and wild boar. It illustrates the symptoms of ASF and lists actions for veterinarians to take should they suspect or confirm ASF on a farm. The objective is to raise awareness of ASF in an effort to curb the global spread of the disease.
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    Brochure, flyer, fact-sheet
    Addressing African swine fever
    Laboratory protocols and algorithms
    2020
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    The Food and Agriculture Organization of the United Nations (FAO) and the World Organisation for Animal Health (OIE) including other partners have been working in countries affected or at risk of incursion by African swine fever (ASF). This document was generated as guidance in response to the emergence of ASF in China, Southeast Asia, and the Pacific. FAO has provided support for laboratory diagnosis of ASF following OIE recommendations, specifically using Polymerase Chain Reaction (PCR) in detecting ASF virus. PCR is a highly sensitive and specific method for the molecular detecting ASF virus for a wide range of purposes, including confirmation of clinical cases and confirmation of freedom from infection before movement. The Australian Centre for Disease Preparedness (ACDP, formerly the Australian Animal Health Laboratories) has developed a diagnostic algorithm based on OIE recommendations and in consultation with the Association of Southeast Asian Nations (ASEAN) regional animal health laboratory network. This document describes a validated real time reverse transcription-polymerase chain reaction (RT-PCR) protocol (the ‘King assay’), which targets the B646L gene, encoding the ASF virus structural protein p72. This assay has been produced in kit form by the ACDP and provided to various veterinary diagnostic laboratories in Southeast Asia by the FAO and OIE. This document also provides links to other reference documents.

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