This paper presents lessons learned and practical guidelines from a workshop on reducing post-harvest losses in grain supply chains in Africa, organised jointly by FAO and the World Bank in 2010 at FAO Headquarters in Rome. Its objectives were to: (i) discuss the significance of post-harvest losses (PHLs) of grains in Africa; (ii) discuss best practices, ongoing activities and institutional arrangements to reduce PHLs of grains; (iii) identify future interventions and define the way forward for future collaboration. The meeting was attended by technical staff from FAO, the World Bank, WFP, IFAD, UNIDO, AfDB, the European Commission and the Natural Resources Institute (NRI) of the UK. Private sector participants from the grain industry included the East African Grain Council, Cereal Growers Association of Kenya, Premium Foods Ltd of Ghana, Grain Traders and Processors Association of Malawi, Food Reserve Agency of Zambia and GrainPro Inc of the USA.