Gender inequality in Palestine has contributed to the marginalization of women from access and control over available resources including water. This inequality has been entrenched through legal, political and social systems. Palestine’s Gender-Responsive Water Assessment was carried out in the West Bank in 2021 by the Food and Agricultural Organization of the United Nations (FAO) in partnership with the Palestinian Authority (PA)- Ministry of Agriculture (MoA) as part of the “Implementing the 2030 Agenda for Water Efficiency, Productivity and Water Sustainability in NENA [North East and North Africa] Countries (GCP/RNE/009/SWE)” project. The project had recommended gender-responsive water assessments to evaluate the relative situation of women and men in different communities regarding water access, governance and use in the countries targeted by the interventions. The aim of the assessment was to outline areas where future investments and programme interventions in water and agriculture would be needed to support progress towards gender equality and sustainable water resource management in a synergistic way. In addition to a review of the existing literature, the study gathered information at the field level, assessing five local communities in the northern West Bank.