This Policy Brief presents a synthesis framework aimed at supporting the EU Partnership on Agroecology Living Labs and Research Infrastructures in its vision to establish an effective European network of living labs. It reaches out to policy and partnership coordinators as well as practitioners, providing insights to inform their decisions on where and which kind of living lab to fund in the future. This decision support can help to fully achieve policy targets related to farmland biodiversity and an agroecological transformation of European farming.