In Nicaragua, gender analysis in value chains is usually restricted to a study of men and women as producers or workers within the chain itself. This overlooks many relevant dimensions of gender struggles. We therefore propose a gender analysis in value chains that pays attention to the interrelation of the value chain with intra-household dynamics in microenterprises and the broader community. We apply our approach to two dairy chains, not to compare which is better for women producers but to show the gender complexity in both that needs to be considered in value chain analyses. Based on case studies, we identify gender differentiation overlapping with conflictual-cooperative relations between men and women within the sphere of economic and family relations in the two dairy chains.