How do you make a convincing ‘business case’ for biodiversity? This guide is for environment officials, interest groups and policymakers to develop the key elements of a case for integrating biodiversity into government and business decisions. The case must appeal to stakeholders in the public and private sectors as well as potential investors and funders. The guide was developed on the basis of experiences shared between members of the African Leadership Group at two NBSAPs 2.0 Mainstreaming Biodiversity and Development project workshops held in Maun, Botswana in November 2012 and Entebbe, Uganda in July 2013.